{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T04:12:07Z","timestamp":1784866327722,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,8,10]],"date-time":"2021-08-10T00:00:00Z","timestamp":1628553600000},"content-version":"vor","delay-in-days":221,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011614","name":"University of Kelaniya","doi-asserted-by":"publisher","award":["RP\/03\/SR\/04\/06\/01\/2016"],"award-info":[{"award-number":["RP\/03\/SR\/04\/06\/01\/2016"]}],"id":[{"id":"10.13039\/501100011614","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>Sri Lanka achieved elimination status for lymphatic filariasis in 2016; still, the disease remains a potential public health issue. The present study is aimed at identifying a subperiodic <jats:italic>Brugia sp.<\/jats:italic> parasite which has reemerged in Sri Lanka after four decades via molecular\u2010based analysis. Polymerase chain reaction performed with pan\u2010filarial primers specific for the internal transcribed spacer region\u20102 (ITS\u20102) of the rDNA of <jats:italic>Brugia<\/jats:italic> filarial parasites isolated from human, canine, and feline blood samples yielded a 615\u2009bp band establishing the species identity as <jats:italic>Brugia malayi<\/jats:italic>. Comparison of the ITS2 sequences of the reemerged <jats:italic>B. malayi<\/jats:italic> isolates with GenBank sequences revealed a higher sequence homology with <jats:italic>B. pahangi<\/jats:italic> than <jats:italic>B. malayi<\/jats:italic> with similar phylogenetic evidence. However, the mean interspecies Kimura\u20102\u2010parameter pairwise divergence between the generated <jats:italic>Brugia<\/jats:italic> sequences with <jats:italic>B. malayi<\/jats:italic> and <jats:italic>B. pahangi<\/jats:italic> was less than 3%. During the analysis of parsimony sites of the new ITS2 sequences, substitutions at A36T, A296G, T373A, and G482A made the sequences different from both <jats:italic>B. pahangi<\/jats:italic> and <jats:italic>B. malayi<\/jats:italic> suggesting the possibility of a new genetic variant or a hybrid strain of <jats:italic>B. malayi<\/jats:italic> and <jats:italic>B. pahangi.<\/jats:italic> Mosquito dissections and xenomonitoring identified <jats:italic>M. uniformis<\/jats:italic> and <jats:italic>M. annulifera<\/jats:italic> as vectors of this novel strain of <jats:italic>B. malayi<\/jats:italic> circulating among cats, dogs, and humans in Sri Lanka.<\/jats:p>","DOI":"10.1155\/2021\/9926101","type":"journal-article","created":{"date-parts":[[2021,8,10]],"date-time":"2021-08-10T16:06:08Z","timestamp":1628611568000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Molecular Characterization of a Reemergent <i>Brugia malayi<\/i> Parasite in Sri Lanka, Suggestive of a Novel Strain"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4238-8931","authenticated-orcid":false,"given":"C. 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