{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T18:04:12Z","timestamp":1759773852885,"version":"3.37.3"},"reference-count":23,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,6,25]],"date-time":"2021-06-25T00:00:00Z","timestamp":1624579200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003459","name":"Guizhou University","doi-asserted-by":"publisher","award":["QianKeHe ZY ZI [2010]LKZ7021","GuiZhongYiBoShiQiDong[2020]75Hao","GNYL [2017] 008","QianJiaoHe KY ZI [2017] 006","QianKeWaiHe G Zi [2010]7012","QianKeHe SY Zi [2009]3104"],"award-info":[{"award-number":["QianKeHe ZY ZI [2010]LKZ7021","GuiZhongYiBoShiQiDong[2020]75Hao","GNYL [2017] 008","QianJiaoHe KY ZI [2017] 006","QianKeWaiHe G Zi [2010]7012","QianKeHe SY Zi [2009]3104"]}],"id":[{"id":"10.13039\/501100003459","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guizhou Province First-Class Discipline Construction Project","award":["QianKeHe ZY ZI [2010]LKZ7021","GuiZhongYiBoShiQiDong[2020]75Hao","GNYL [2017] 008","QianJiaoHe KY ZI [2017] 006","QianKeWaiHe G Zi [2010]7012","QianKeHe SY Zi [2009]3104"],"award-info":[{"award-number":["QianKeHe ZY ZI [2010]LKZ7021","GuiZhongYiBoShiQiDong[2020]75Hao","GNYL [2017] 008","QianJiaoHe KY ZI [2017] 006","QianKeWaiHe G Zi [2010]7012","QianKeHe SY Zi [2009]3104"]}]},{"name":"Syndrome Pharmacology in Chinese Medicine","award":["QianKeHe ZY ZI [2010]LKZ7021","GuiZhongYiBoShiQiDong[2020]75Hao","GNYL [2017] 008","QianJiaoHe KY ZI [2017] 006","QianKeWaiHe G Zi [2010]7012","QianKeHe SY Zi [2009]3104"],"award-info":[{"award-number":["QianKeHe ZY ZI [2010]LKZ7021","GuiZhongYiBoShiQiDong[2020]75Hao","GNYL [2017] 008","QianJiaoHe KY ZI [2017] 006","QianKeWaiHe G Zi [2010]7012","QianKeHe SY Zi [2009]3104"]}]},{"name":"Guizhou Province International Science and Technology Cooperation Project","award":["QianKeHe ZY ZI [2010]LKZ7021","GuiZhongYiBoShiQiDong[2020]75Hao","GNYL [2017] 008","QianJiaoHe KY ZI [2017] 006","QianKeWaiHe G Zi [2010]7012","QianKeHe SY Zi [2009]3104"],"award-info":[{"award-number":["QianKeHe ZY ZI [2010]LKZ7021","GuiZhongYiBoShiQiDong[2020]75Hao","GNYL [2017] 008","QianJiaoHe KY ZI [2017] 006","QianKeWaiHe G Zi [2010]7012","QianKeHe SY Zi [2009]3104"]}]},{"name":"Guizhou Province Science and Technology Plan Project Tasks","award":["QianKeHe ZY ZI [2010]LKZ7021","GuiZhongYiBoShiQiDong[2020]75Hao","GNYL [2017] 008","QianJiaoHe KY ZI [2017] 006","QianKeWaiHe G Zi [2010]7012","QianKeHe SY Zi [2009]3104"],"award-info":[{"award-number":["QianKeHe ZY ZI [2010]LKZ7021","GuiZhongYiBoShiQiDong[2020]75Hao","GNYL [2017] 008","QianJiaoHe KY ZI [2017] 006","QianKeWaiHe G Zi [2010]7012","QianKeHe SY Zi [2009]3104"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2021,6,25]]},"abstract":"<jats:p>Aim. Traditionally, C. sappan medicine was the heartwood, which needs to be cut down as a whole. In this research, the antitumor activity and mechanisms of the leaves and stems were compared with the roots of Caesalpinia sappan; it was in order to investigate whether stems and leaves of C. sappan could be used to replace heartwood for antitumor treatment, thereby reducing resource destruction. Methods. MTT assays were used to identify the active sites of C. sappan based on the application of human liver cancer (HuH-7) cells. High-performance liquid chromatography (HPLC) was used to analyze polar extracts. We also established a H22 hepatoma-bearing mouse model by administering intraperitoneal injections of petroleum ether extracts from the leaves and stems (SY\u2461) at doses of 20 and 65\u2009mg\/kg. Mice in the i.g. group were administered intragastrically with the same extracts (at doses of 100 and 325\u2009mg\/kg) at the same time (12\u2009days). Results. The antitumor site of C. sappan was the petroleum ether extract. The IC50 for the petroleum ether extract of roots (SG\u2461) was 56.10\u2009\u03bcg\/ml, while that for the leaves and stems (SY\u2461) was 77.20\u2009\u03bcg\/ml. Grey relational analysis indicated 11 active fraction peaks that were closely related to antitumor activity. The size of tumors in H22 hepatoma-bearing mice was reduced significantly in mice administered with petroleum ether extracts from the leaves and stems (inhibition rates of high doses were 55.31% and 60.56%). Fibrous tissue proliferation, inflammatory cell infiltration, tumor cell necrosis, and the expression of proliferating cell nuclear antigen (PCNA) and vascular endothelial growth factor (VEGF) were all lower than in the control group (VEGF <jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>P<\/a:mi>\n                     <\/a:math>\n                  <\/jats:inline-formula>\u2009&lt;\u20090.001 and PCNA <jats:inline-formula>\n                     <c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                        <c:mi>P<\/c:mi>\n                     <\/c:math>\n                  <\/jats:inline-formula>\u2009&lt;\u20090.05). Conclusion. Petroleum ether extracts of the roots, leaves, and stems of C. sappan exhibit certain antitumor effects. Our data indicate that the mechanisms underlying these effects may relate to a reduction in the expression of PCNA and VEGF and the inhibition of angiogenesis. Our findings indicate that we can expand the medicinal use of C. sappan to the leaves and stems, thus improving resource utilization and reducing resource damage.<\/jats:p>","DOI":"10.1155\/2021\/9939345","type":"journal-article","created":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T19:07:03Z","timestamp":1624907223000},"page":"1-13","source":"Crossref","is-referenced-by-count":6,"title":["Activity Screening of the Herb Caesalpinia sappan and an Analysis of Its Antitumor Effects"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3680-4831","authenticated-orcid":true,"given":"Yuan","family":"Li","sequence":"first","affiliation":[{"name":"Guizhou University of Traditional Chinese Medicine, Guian District, Guiyang 550025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4517-8799","authenticated-orcid":true,"given":"Minghong","family":"Dong","sequence":"additional","affiliation":[{"name":"Guizhou University of Traditional Chinese Medicine, Guian District, Guiyang 550025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7847-4543","authenticated-orcid":true,"given":"Zijun","family":"Wu","sequence":"additional","affiliation":[{"name":"Tianjin University of Traditional Chinese Medicine, Jinghai District, Tianjin 301699, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1215-0914","authenticated-orcid":true,"given":"Yongqi","family":"Huang","sequence":"additional","affiliation":[{"name":"Guizhou University of Traditional Chinese Medicine, Guian District, Guiyang 550025, China"},{"name":"Guizhou Province Key Laboratory of Prescription and Syndrome Pharmacology in Chinese Medicine, Guian District, Guiyang 550025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7687-6218","authenticated-orcid":true,"given":"Haibing","family":"Qian","sequence":"additional","affiliation":[{"name":"Guizhou University of Traditional Chinese Medicine, Guian District, Guiyang 550025, China"},{"name":"Guizhou Province Key Laboratory of Prescription and Syndrome Pharmacology in Chinese Medicine, Guian District, Guiyang 550025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4894-6623","authenticated-orcid":true,"given":"Cong","family":"Huang","sequence":"additional","affiliation":[{"name":"Guizhou University of Traditional Chinese Medicine, Guian District, Guiyang 550025, China"},{"name":"Guizhou Province Key Laboratory of Prescription and Syndrome Pharmacology in Chinese Medicine, Guian District, Guiyang 550025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.19482\/j.cn11-3237.2019.05.08"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1008-0805.2011.05.103"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.3321\/j.issn:1001-4454.2007.02.030"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/S1875-5364(14)60092-3"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.11655\/zgywylc2015.08.011"},{"issue":"3","key":"6","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1002\/iub.1357","article-title":"c-Fos is involved in inhibition of human bladder carcinoma T24 cells by brazilin","volume":"67","author":"T. Zhang","year":"2015","journal-title":"IUBMB Life"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/864892"},{"issue":"2","key":"8","doi-asserted-by":"crossref","first-page":"2449","DOI":"10.3390\/molecules18022449","article-title":"Brazilin inhibits growth and induces apoptosis in human glioblastoma cells","volume":"18","author":"D. Y. Lee","year":"2013","journal-title":"Molecules"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1177\/1534735415588929"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.114.218792"},{"issue":"24","key":"11","first-page":"3623","article-title":"Chemical constituents of triterpenoids from Prunella vulgaris and their antitumor activities","volume":"46","author":"Y. B. Bai","year":"2015","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.7501\/j.issn.0253-2670.2018.12.025"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.14148\/j.issn.1672-0482.2019.0664"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1177\/0300985819854439"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.13422\/j.cnki.syfjx.2015010121"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.13422\/j.cnki.syfjx.20182492"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.13422\/j.cnki.syfjx.20191215"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.4268\/cjcmm201517205"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejbas.2017.07.006"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1177\/0300985816646429"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1672-1993.2018.08.004"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.13748\/j.cnki.issn1007-7693.2020.05.011"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.25.2.335"}],"container-title":["Evidence-Based Complementary and Alternative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2021\/9939345.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2021\/9939345.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2021\/9939345.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T19:07:25Z","timestamp":1624907245000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/ecam\/2021\/9939345\/"}},"subtitle":[],"editor":[{"given":"Yu","family":"CAI","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,6,25]]},"references-count":23,"alternative-id":["9939345","9939345"],"URL":"https:\/\/doi.org\/10.1155\/2021\/9939345","relation":{},"ISSN":["1741-4288","1741-427X"],"issn-type":[{"type":"electronic","value":"1741-4288"},{"type":"print","value":"1741-427X"}],"subject":[],"published":{"date-parts":[[2021,6,25]]}}}