{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T14:47:42Z","timestamp":1783435662126,"version":"3.54.6"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,8,24]],"date-time":"2021-08-24T00:00:00Z","timestamp":1629763200000},"content-version":"vor","delay-in-days":235,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Major science and technology projects of Yunnan Province","award":["202002 AA100004"],"award-info":[{"award-number":["202002 AA100004"]}]},{"name":"Major science and technology projects of Yunnan Province","award":["2019ZX09301-156"],"award-info":[{"award-number":["2019ZX09301-156"]}]},{"name":"National Science Technology Major Project of China","award":["202002 AA100004"],"award-info":[{"award-number":["202002 AA100004"]}]},{"name":"National Science Technology Major Project of China","award":["2019ZX09301-156"],"award-info":[{"award-number":["2019ZX09301-156"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p><jats:italic>Objectives<\/jats:italic>. <jats:italic>Dendrobium catenatum Lindl<\/jats:italic>. (DH) is a Chinese herbal medicine, which is often used to make tea to improve immunity in China. Rumor has it that DH has a protective effect against cardiovascular disease. However, it is not clear how DH can prevent cardiovascular disease, such as atherosclerosis (AS). Therefore, the purpose of this study is to study whether DH can prevent AS and the underlying mechanisms. <jats:italic>Methods<\/jats:italic>. Zebrafish larvae were fed with high\u2010cholesterol diet (HCD) to establish a zebrafish AS model. Then, we used DH water extracts (DHWE) to pretreat AS zebrafish. The plaque formation was detected by HE, EVG, and oil red O staining. Neutrophil and macrophage counts were calculated to evaluate the inflammation level. Reactive oxygen species (ROS) activity, malondialdehyde (MDA) content, and superoxide dismutase (SOD) activity in zebrafish were measured to reflect oxidative stress. The cholesterol accumulation and the levels of lipid, triglyceride (TG), and total cholesterol (TC) were measured to reflect lipid metabolism disorder. Then, parallel flow chamber was utilized to establish a low shear stress\u2010 (LSS\u2010) induced endothelial cell (EC) dysfunction model. EA.hy926 cells were exposed to LSS (3\u2009dyn\/cm<jats:sup>2<\/jats:sup>) for 30\u2009min and treated with DHWE. The levels of ROS, SOD, MDA, glutathione (GSH), and glutathiol (GSSG) in EA.hy926 cells were analysed to determine oxidative stress. The release of nitric oxide (NO), endothelin\u20101 (ET\u20101), and epoprostenol (PGI<jats:sub>2<\/jats:sub>) in EA.hy926 cells was measured to reflect EC dysfunction. The mRNA expression of intercellular adhesion molecule\u20101 (ICAM\u20101) and vascular cell adhesion molecule\u20101 (VCAM\u20101) in EA.hy926 cells was detected to reflect EC dysfunction inflammation. <jats:italic>Results<\/jats:italic>. The results showed that DHWE significantly reduced cholesterol accumulation and macrophage infiltration in early AS. Finally, DHWE significantly alleviate the lipid metabolism disorder, oxidative stress, and inflammation to reduce the plaque formation of AS zebrafish larval model. Meanwhile, we also found that DHWE significantly improved LSS\u2010induced EC dysfunction and oxidative stress <jats:italic>in vitro<\/jats:italic>. <jats:italic>Conclusion<\/jats:italic>. Our results indicate that DHWE could be used as a prevention method to prevent AS.<\/jats:p>","DOI":"10.1155\/2021\/9951946","type":"journal-article","created":{"date-parts":[[2021,8,24]],"date-time":"2021-08-24T14:17:51Z","timestamp":1629814671000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["<i>Dendrobium catenatum Lindl.<\/i> Water Extracts Attenuate 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