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Decreased expression of TLR homolog CD180 in peripheral blood B cells and its potential role in antibody production have been described in autoimmune diseases. Effectiveness of anti-CD20 therapy in neuromyelitis optica spectrum disorder (NMOSD) and multiple sclerosis (MS) strengthens the role of B cells in the pathogenesis. Therefore, we aimed to investigate the CD180 expression of peripheral blood B cell subsets in NMOSD and MS patients and analyze the levels of natural anti-citrate synthase (CS) IgG autoantibodies and IgG antibodies induced by bacterial infections reported to play a role in the pathogenesis of NMOSD or MS. Methods. We analyzed the distribution and CD180 expression of peripheral blood B cell subsets, defined by CD19\/CD27\/IgD staining, and measured anti-CS IgM\/G natural autoantibody and antibacterial IgG serum levels in NMOSD, RRMS, and healthy controls (HC). Results. We found decreased na\u00efve and increased memory B cells in NMOSD compared to MS. Among the investigated four B cell subsets, CD180 expression was exclusively decreased in CD19+CD27+IgD+ nonswitched (NS) memory B cells in both NMOSD and MS compared to HC. Furthermore, the anti-CS IgM natural autoantibody serum level was lower in both NMOSD and MS. In addition, we found a tendency of higher anti-CS IgG natural autoantibody levels only in anti-Chlamydia IgG antibody-positive NMOSD and MS patients. Conclusions. Our results suggest that reduced CD180 expression of NS B cells could contribute to the deficient natural IgM autoantibody production in NMOSD and MS, whereas natural IgG autoantibody levels show an association with antibacterial antibodies.<\/jats:p>","DOI":"10.1155\/2021\/9953317","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T23:05:20Z","timestamp":1621983920000},"page":"1-11","source":"Crossref","is-referenced-by-count":6,"title":["Toll-Like Receptor Homolog CD180 Expression Is Diminished on Natural Autoantibody-Producing B Cells of Patients with Autoimmune CNS Disorders"],"prefix":"10.1155","volume":"2021","author":[{"given":"Zs\u00f3fia","family":"Hayden","sequence":"first","affiliation":[{"name":"Department of Immunology and Biotechnology, Clinical Center, University of P\u00e9cs Medical School, H-7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Szabina","family":"Erd\u0151-Bony\u00e1r","sequence":"additional","affiliation":[{"name":"Department of Immunology and Biotechnology, Clinical Center, University of P\u00e9cs Medical School, H-7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2668-4300","authenticated-orcid":true,"given":"Be\u00e1ta","family":"B\u00f3n\u00e9","sequence":"additional","affiliation":[{"name":"Department of Neurology, Clinical Center, University of P\u00e9cs Medical School, H-7623 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"No\u00e9mi","family":"Bal\u00e1zs","sequence":"additional","affiliation":[{"name":"Department of Immunology and Biotechnology, Clinical Center, University of P\u00e9cs Medical School, H-7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Korn\u00e9lia","family":"Bod\u00f3","sequence":"additional","affiliation":[{"name":"Department of Immunology and Biotechnology, Clinical Center, University of P\u00e9cs Medical School, H-7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zsolt","family":"Illes","sequence":"additional","affiliation":[{"name":"Department of Neurology, Odense University Hospital, Denmark"},{"name":"Brain Research Inter-Disciplinary Guided Excellence, Department of Clinical Research, Faculty of Health Sciences, University of Southern Denmark, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timea","family":"Berki","sequence":"additional","affiliation":[{"name":"Department of Immunology and Biotechnology, Clinical Center, University of P\u00e9cs Medical School, H-7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Di\u00e1na","family":"Simon","sequence":"additional","affiliation":[{"name":"Department of Immunology and Biotechnology, Clinical Center, University of P\u00e9cs Medical School, H-7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1111\/cei.12878"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0235449"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-019-1593-2"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/460825"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1093\/braincomms\/fcaa197"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1007\/s40120-018-0101-4"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.msard.2019.101397"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1007\/s11940-015-0387-9"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(07)70216-8"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00201"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2012249117"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1810470115"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.2VMR1215-582R"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1038\/ni1198"},{"issue":"1","key":"15","first-page":"5","article-title":"Increase of RP105-lacking activated B cells in the peripheral blood and salivary glands in patients with Sj\u00f6gren's syndrome","volume":"26","author":"Y. 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