{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T22:41:50Z","timestamp":1789512110248,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,12,16]],"date-time":"2021-12-16T00:00:00Z","timestamp":1639612800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2021,12,16]]},"abstract":"<jats:p>Objective. To clarify the function of miRNA-19b-3p in accelerating myocardial ischemia-reperfusion injury- (MIRI-) induced cardiomyocyte apoptosis by downregulating gene of phosphate and tension homology deleted on chromsome ten (PTEN), thus influencing the progression of acute myocardial infarction. Materials and Methods. miRNA-19b-3p and PTEN levels in HCM cells undergoing hypoxia\/reoxygenation (H\/R) were determined. Meanwhile, activities of myocardium injury markers [lactate dehydrogenase (LDH), malondialdehyde; malonic dialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX)] in H\/R-induced HCM cells were tested. Through dual-luciferase reporter gene assay, the binding between miRNA-19b-3p and PTEN was verified. Regulatory effects of miRNA-19b-3p and PTEN on apoptotic rate and apoptosis-associated gene expressions (proapoptotic protein Bcl-2 associated X protein (Bax), antiapoptotic protein B-cell lymphoma-2 (Bcl-2), and cytochrome C) in H\/R-induced human cardiac myocytes (HCM) cells were examined. Results. miRNA-19b-3p was upregulated, while PTEN was downregulated in H\/R-induced HCM cells. Knockdown of miRNA-19b-3p decreased activities of LDH, MDA, and GSH-PX, but increased SOD level in H\/R-induced HCM cells. The binding between miRNA-19b-3p and PTEN was confirmed. More importantly, knockdown of miRNA-19b-3p reduced apoptotic rate, downregulated proapoptosis gene expressions (Bax and cytochrome C), and upregulated antiapoptosis gene expression (Bcl-2), which were reversed by silence of PTEN. Conclusions. miRNA-19b-3p is upregulated in HCM cells undergoing hypoxia and reoxygenation, which accelerates MIRI-induced cardiomyocyte apoptosis through downregulating PTEN.<\/jats:p>","DOI":"10.1155\/2021\/9956666","type":"journal-article","created":{"date-parts":[[2021,12,16]],"date-time":"2021-12-16T21:35:08Z","timestamp":1639690508000},"page":"1-7","source":"Crossref","is-referenced-by-count":7,"title":["miRNA-19b-3p Stimulates Cardiomyocyte Apoptosis Induced by Myocardial Ischemia Reperfusion via Downregulating PTEN"],"prefix":"10.1155","volume":"2021","author":[{"given":"Ke","family":"Li","sequence":"first","affiliation":[{"name":"Department of Cardiology, Nanyang Second People\u2019s Hospital, Nanyang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xujie","family":"Ya","sequence":"additional","affiliation":[{"name":"The First Department of Cardiology, The Eighth People\u2019s Hospital of Hengshui City, Hengshui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiujuan","family":"Duan","sequence":"additional","affiliation":[{"name":"The Second Department of Cardiology, The Eighth People\u2019s Hospital of Hengshui City, Hengshui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine, The First Affiliated Hospital of Baotou Mdical College, Baotou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6544-1462","authenticated-orcid":true,"given":"Xuefeng","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine, The First Affiliated Hospital of Baotou Mdical College, Baotou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.05.082"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_201804_14833"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2017.04.001"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112160"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(98)00033-9"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra071667"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2018.03.006"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1996.0193"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.2007.01878.x"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.183087"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1172\/JCI33099"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1038\/nature01957"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1126\/science.1062961"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-19138-4"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmhg.2015.03.006"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1159\/000489383"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1093\/europace\/eux289"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/1957920"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2019.10266"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.116619"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2017.3164"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1007\/s10517-017-3620-6"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.7150\/thno.10305"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-016-0465-1"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.14311"},{"issue":"1","key":"26","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1038\/s41418-019-0349-y","article-title":"Exosomal miRNA-19b-3p of tubular epithelial cells promotes M1 macrophage activation in kidney injury","volume":"27","author":"L. 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