{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T10:55:06Z","timestamp":1791888906075,"version":"4.3.4"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T00:00:00Z","timestamp":1637020800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31370378"],"award-info":[{"award-number":["31370378"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2021,11,16]]},"abstract":"<jats:p>Background. Traditional Chinese medicine Smilax is the rhizome of liliaceous plant Smilax china L., which is used to treat pelvic inflammatory disease and anxieties. Purpose. To investigate the mechanism of anti-inflammatory activity of the extract from Smilax china L. (ES). Methods. The components of ES were identified by UPLC-QTOF-MS\/MS. The anti-inflammatory activities were evaluated in xylene-induced ear oedema and egg white-induced plantar swelling test. Cell viability was examined by CCK-8 assay. The inflammatory mediators, proinflammatory cytokines, and MAPK and NF-\u03baB signals in LPS-stimulated THP-1 cells were determined using ELISA, real-time PCR, and Western blot, respectively. Results. 20 compounds of ES were confirmed by comparing with the reference substance. ES displayed more prominent anti-inflammatory activity than the positive control \u201cJin Gang Teng\u201d capsule in the in vivo acute inflammatory model. ES suppressed the expression of PGE2 and 6-Keot-PGF1\u03b1, and the ratio of IC50 (COX-1)\/IC50 (COX-2) of ES was 3.15, which indicated that ES could selectively inhibit COX-2. ES dose-dependently (12.5, 25, and 50\u2009mg\/L) decreased the production and mRNA levels of proinflammatory cytokines IL-1\u03b2, IL-6, and TNF-\u03b1. Furthermore, ES significantly decreased LPS-induced phosphorylation of p38, JNK, ERK1\/2, and p65, inhibiting the expression of IKK\u03b1 and the degradation of I\u03baB\u03b1. Conclusion. The results suggested that ES could selectively inhibit the activity of COX-2, and the anti-inflammatory effect of ES was associated with the inhibition of IL-1\u03b2, IL-6, and TNF-\u03b1 via negative regulation of MAPK and NF-\u03baB signaling pathways in LPS-induced THP-1 cells.<\/jats:p>","DOI":"10.1155\/2021\/9958808","type":"journal-article","created":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T22:20:12Z","timestamp":1637101212000},"page":"1-12","source":"Crossref","is-referenced-by-count":8,"title":["The Anti-Inflammatory Effect of Smilax china L. Extract on LPS-Stimulated THP-1 via Downregulation of MAPK and NF-\u03baB Signaling Pathway"],"prefix":"10.1155","volume":"2021","author":[{"given":"Siyi","family":"Jiang","sequence":"first","affiliation":[{"name":"College of Pharmacy of Hubei University of Chinese Medicine, Wuhan, Hubei 430061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Pharmacy of Hubei University of Chinese Medicine, Wuhan, Hubei 430061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4328-478X","authenticated-orcid":true,"given":"Xiaochuan","family":"Ye","sequence":"additional","affiliation":[{"name":"College of Pharmacy of Hubei University of Chinese Medicine, Wuhan, Hubei 430061, China"},{"name":"Hubei Province Key Laboratory of Traditional Chinese Medicine Resource and Chemistry, Wuhan, Hubei 430061, 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