{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T21:11:39Z","timestamp":1781212299361,"version":"3.54.1"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,11,27]],"date-time":"2021-11-27T00:00:00Z","timestamp":1637971200000},"content-version":"vor","delay-in-days":330,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003996","name":"Kunming Medical University","doi-asserted-by":"publisher","award":["CXTD2020xx"],"award-info":[{"award-number":["CXTD2020xx"]}],"id":[{"id":"10.13039\/501100003996","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007846","name":"Yunnan Provincial Department of Education","doi-asserted-by":"publisher","award":["2019Y0364"],"award-info":[{"award-number":["2019Y0364"]}],"id":[{"id":"10.13039\/501100007846","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82160177"],"award-info":[{"award-number":["82160177"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81760193"],"award-info":[{"award-number":["81760193"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81960195"],"award-info":[{"award-number":["81960195"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p><jats:italic>Objectives<\/jats:italic>. Combining the advantages of static magnetic fields (SMF) and coculture systems, we investigated the effect of moderate\u2010intensity SMF on the chondrogenesis and proliferation of mandibular bone marrow mesenchymal stem cells (MBMSCs) in the MBMSC\/mandibular condylar chondrocyte (MCC) coculture system. The main aim of the present study was to provide an experimental basis for obtaining better cartilage tissue engineering seed cells for the effective repair of condylar cartilage defects in clinical practice. <jats:italic>Methods<\/jats:italic>. MBMSCs and MCCs were isolated from SD (Sprague Dawley) rats. Flow cytometry, three\u2010lineage differentiation, colony\u2010forming assays, immunocytochemistry, and toluidine blue staining were used for the identification of MBMSCs and MCCs. MBMSCs and MCCs were seeded into the lower and upper Transwell chambers, respectively, at a ratio of 1\u2009:\u20092, and exposed to a 280\u2009mT SMF. MBMSCs were harvested after 3, 7, or 14 days for analysis. CCK\u20108 was used to detect cell proliferation, Alcian blue staining was utilized to evaluate glycosaminoglycan (GAG), and western blotting and real\u2010time quantitative polymerase chain reaction (RT\u2010qPCR) detected protein and gene expression levels of SOX9, Col2A1 (Collagen Type II Alpha 1), and Aggrecan (ACAN). <jats:italic>Results<\/jats:italic>. The proliferation of MBMSCs was significantly enhanced in the experimental group with MBMSCs cocultured with MCCs under SMF stimulation relative to controls (<jats:italic>P<\/jats:italic> &lt; 0.05). GAG content was increased, and SOX9, Col2A1, and ACAN were also increased at the mRNA and protein levels (<jats:italic>P<\/jats:italic> &lt; 0.05). <jats:italic>Conclusions<\/jats:italic>. Moderate\u2010intensity SMF improved the chondrogenesis and proliferation of MBMSCs in the coculture system, and it might be a promising approach to repair condylar cartilage defects in the clinical setting.<\/jats:p>","DOI":"10.1155\/2021\/9962861","type":"journal-article","created":{"date-parts":[[2021,11,27]],"date-time":"2021-11-27T18:05:08Z","timestamp":1638036308000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Static Magnetic Fields Enhance the Chondrogenesis of Mandibular Bone Marrow Mesenchymal Stem Cells in Coculture Systems"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0704-4316","authenticated-orcid":false,"given":"Ming","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5829-3887","authenticated-orcid":false,"given":"Weihao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1185-1014","authenticated-orcid":false,"given":"Wei","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9780-0527","authenticated-orcid":false,"given":"Yanhua","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,27]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1177\/1947603520926711"},{"key":"e_1_2_9_2_2","first-page":"151","article-title":"The morphology and selected biological properties of articular cartilage","volume":"3","author":"Hyc A.","year":"2001","journal-title":"Ortopedia, Traumatologia, Rehabilitacja"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/0022034515574770"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/boneres.2015.40"},{"key":"e_1_2_9_5_2","first-page":"50","article-title":"Physical and medicinal treatment of TMJ arthrosis","volume":"87","author":"Lalabonova K.","year":"2008","journal-title":"Stomatologiia"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109146"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.0150"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.23216"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1039\/D0TB00172D"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.5114\/aoms.2016.59894"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.8.1671"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2008.0007"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510378427"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/term.2171"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.4103\/0976-500X.155481"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2011000400006"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26020"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.12.065"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.30086"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1815447116"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.22-3-313"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2008.09.004"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2014.02.001"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2002.17.10.1814"},{"key":"e_1_2_9_25_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2013.0307"},{"key":"e_1_2_9_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-09892-w"},{"key":"e_1_2_9_27_2","doi-asserted-by":"publisher","DOI":"10.3109\/03008207.2011.620190"},{"key":"e_1_2_9_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/os.12603"},{"key":"e_1_2_9_29_2","first-page":"147","article-title":"Isolation and cultivation of mandibular bone marrow mesenchymal stem cells in rats","volume":"162","author":"Hong Y.","year":"2020","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_9_30_2","article-title":"Low-intensity pulsed ultrasound modulates RhoA\/ROCK signaling of rat mandibular bone marrow mesenchymal stem cells to rescue their damaged cytoskeletal organization and cell biological function induced by radiation","volume":"2","author":"Zhang R.","year":"2020","journal-title":"Stem Cells International"},{"key":"e_1_2_9_31_2","doi-asserted-by":"publisher","DOI":"10.1159\/000093061"},{"key":"e_1_2_9_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/bem.22012"},{"key":"e_1_2_9_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/bem.22009"},{"key":"e_1_2_9_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2020.04.090"},{"key":"e_1_2_9_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/clr.12812"},{"key":"e_1_2_9_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-015-5659-0"},{"key":"e_1_2_9_37_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2017.0162"},{"key":"e_1_2_9_38_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2008.0329"},{"key":"e_1_2_9_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199811)177:2<343::AID-JCP16>3.0.CO;2-A"},{"key":"e_1_2_9_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2017.03.031"},{"key":"e_1_2_9_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3151"},{"key":"e_1_2_9_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2013.03.010"},{"key":"e_1_2_9_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2015.170"},{"key":"e_1_2_9_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.24495"},{"key":"e_1_2_9_45_2","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2018.8195"},{"key":"e_1_2_9_46_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2008-0512"},{"key":"e_1_2_9_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2012.12.007"},{"key":"e_1_2_9_48_2","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.072490gh"},{"key":"e_1_2_9_49_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.00353"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2021\/9962861.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2021\/9962861.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2021\/9962861","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T06:53:44Z","timestamp":1735628024000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2021\/9962861"}},"subtitle":[],"editor":[{"given":"Jangho","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":49,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1155\/2021\/9962861"],"URL":"https:\/\/doi.org\/10.1155\/2021\/9962861","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"2021-03-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"9962861"}}