{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T17:32:03Z","timestamp":1792431123689,"version":"4.5.0"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,10,28]],"date-time":"2021-10-28T00:00:00Z","timestamp":1635379200000},"content-version":"vor","delay-in-days":300,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81774208"],"award-info":[{"award-number":["81774208"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Yinchen Wuling powder is often used to treat clinical hyperlipidemia, although its mechanism of action remains unclear. In this study, we aimed to investigate the active ingredients found in Yinchen Wuling powder and find its mechanism of action when treating hyperlipidemia, using a combination of network pharmacology, molecular docking, and molecular dynamics simulation approaches. <jats:italic>Methods<\/jats:italic>. The TCMSP database was used to obtain the principle active ingredients found in Yinchen Wuling powder and the NCBI and DisGeNet databases were used to obtain the main target genes involved in hyperlipidemia, and the intersectional targets were obtained by EXCEL. We also used Cytoscape 3.7.2 software to construct a \u201cTraditional Chinese Medicine\u2010Active Ingredient\u2010Target\u201d network and use STRING platform to conduct \u201cprotein\u2010protein interactional\u201d (PPI) analyses on the intersection targets. Bioconductor software and RX 64 4.0.0 software were then used to perform GO functional enrichment analysis and KEGG pathway enrichment analysis on the targets. Molecular docking of core protein\u2010ligand interactions was modeled using AutoDock Vina software. A simulation of molecular dynamics was conducted for the optimal core protein\u2010ligand obtained by molecular docking using Amber18 software. <jats:italic>Results<\/jats:italic>. A total of 63 active ingredients were found in Yinchen Wuling powder, corresponding to 175 targets, 508 hyperlipidemia targets, and 55 intersection targets in total. Cytoscape 3.7.2 showed that the key active ingredients were quercetin, isorhamnetin, taxifolin, demethoxycapillarisin, and artepillin A. The PPI network showed that the key proteins involved were AKT1, IL6, VEGFA, and PTGS2. GO enrichment analysis found that genes were enriched primarily in response to oxygen levels and nutrient levels of the vesicular lumen and were associated with membrane rafts. These were mainly enriched in AGE\u2010RAGE (advanced glycation end products\u2010receptor for advanced glycation end products) signaling pathway in diabetic complications, fluid shear stress, and atherosclerosis, as well as other pathways. The molecular docking results indicated key binding activity between PTGS2\u2010quercetin, PTGS2\u2010isorhamnetin, and PTGS2\u2010taxifolin. Results from molecular dynamics simulations showed that PTGS2\u2010quercetin, PTGS2\u2010isorhamnetin, and PTGS2\u2010taxifolin bound more stably, and their binding free energies were PTGS2\u2010quercetin \u201029.5\u2009kcal\/mol, PTGS2\u2010isorhamnetin \u201032\u2009kcal\/mol, and PTGS2\u2010taxifolin \u201032.9\u2009kcal\/mol. <jats:italic>Conclusion<\/jats:italic>. This study is based on network pharmacology and reveals the potential molecular mechanisms involved in the treatment of hyperlipidemia by Yinchen Wuling powder.<\/jats:p>","DOI":"10.1155\/2021\/9965906","type":"journal-article","created":{"date-parts":[[2021,10,28]],"date-time":"2021-10-28T19:50:19Z","timestamp":1635450619000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":108,"title":["Exploring the Molecular Mechanism of Action of Yinchen Wuling Powder for the Treatment of Hyperlipidemia, Using Network Pharmacology, Molecular Docking, and Molecular Dynamics Simulation"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5035-9770","authenticated-orcid":false,"given":"Jiahao","family":"Ye","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5958-0666","authenticated-orcid":false,"given":"Lin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2316-8645","authenticated-orcid":false,"given":"Zhixi","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,10,28]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2018.09.024"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000013048"},{"key":"e_1_2_10_3_2","first-page":"642","article-title":"Mechanism of Yinchen Wuling powder in treating hyperlipidemia Guangming","volume":"24","author":"Lin S. C.","year":"2009","journal-title":"Journal of Chinese Medicine"},{"key":"e_1_2_10_4_2","article-title":"Clinical observation on treatment of primary hyperlipidemia with Yinchen Wuling powder","volume":"4","author":"Yan H. X.","year":"1998","journal-title":"Henan Traditional Chinese Medicine"},{"key":"e_1_2_10_5_2","article-title":"Modified Yinchen Wuling powder in treating diabetic hyperlipemia","volume":"33","author":"Zhang N.","year":"2011","journal-title":"Hubei Journal of Traditional Chinese Medicine"},{"key":"e_1_2_10_6_2","first-page":"2606","article-title":"Clinical study on Yinchen Wuling powder in treatment of hemodialysis patients with hyperlipidemia","volume":"41","author":"Ma H. J.","year":"2014","journal-title":"Liaoning Journal of Traditional Chinese Medicine"},{"key":"e_1_2_10_7_2","first-page":"259","article-title":"Lipid modulation and anticoagulative function of yinchenwulin powder on hyperlipidemia rats","volume":"36","author":"Li R. M.","year":"2016","journal-title":"Chinese Journal of Gerontology"},{"key":"e_1_2_10_8_2","first-page":"3639","article-title":"Research on regulating LDL-C in hyperlipidemia rats through p38 MAPK, p42\/44 MAPK pathways with Yinchen Wuling powder","volume":"30","author":"Wu N.","year":"2015","journal-title":"China Journal of Traditional Chinese Medicine and Pharmacy"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2013.04.036"},{"key":"e_1_2_10_10_2","first-page":"1028","article-title":"Experimental study on the antihtrombotic activity and regulating effect in blood lipids of safflor yellow","volume":"14","author":"Zhang H. Y.","year":"2010","journal-title":"Chinese Journal of Experimental Traditional Medical Formulae"},{"key":"e_1_2_10_11_2","first-page":"599","article-title":"Effect of p-hydroxyacetophenone on lowering blood lipids","volume":"31","author":"Zhang T.","year":"2010","journal-title":"World Clinical Drugs"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2010.08.021"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.4062\/biomolther.2013.100"},{"key":"e_1_2_10_14_2","first-page":"1760","article-title":"Experimental study on the pharmacological effects of Polyporus umbellatus polysaccharide on reducing blood lipids and resisting alcoholic fatty liver in rats","volume":"35","author":"Liu X. L.","year":"2013","journal-title":"Chinese Traditional Patent Medicine"},{"key":"e_1_2_10_15_2","article-title":"Study on TLC identification and UPLC determination method of atractylenolide in Atractylodes macrocephala","volume":"42","author":"Zhao Y. J.","year":"2017","journal-title":"China Journal of Chinese Materia Medica"},{"key":"e_1_2_10_16_2","first-page":"4","article-title":"Effect of volatile oil from Atractylodes macrocephala Koidz on the glucolipid metabolism of metabolic syndrome in rats","volume":"14","author":"Su Z. Q.","year":"2018","journal-title":"Asia-Pacific Traditional Medicine"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms161125944"},{"key":"e_1_2_10_18_2","article-title":"Hypolipidemic effects of Alismatis rhizome on lipid profile in mice fed high-fat diet","volume":"32","author":"Hong D.","year":"2011","journal-title":"Saudi Medical Journal"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/1749-8546-4-5"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/4753852"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.4103\/wjtcm.wjtcm_6_18"},{"key":"e_1_2_10_22_2","first-page":"154","article-title":"Mechanism of action of Trichosanthis Fructus-Allii Macrostemonis Bulbus herb pairs against hyperlipidemia based on network pharmacology","volume":"26","author":"Zhong H.","year":"2020","journal-title":"Chinese Journal of Experimental Traditional Medical Formulae"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.109.192535"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/1758-2946-6-13"},{"key":"e_1_2_10_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1758-4"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1003"},{"key":"e_1_2_10_27_2","article-title":"An automated method for finding molecular complexes in large protein interaction networks","volume":"4","author":"Bader G. D.","year":"2003","journal-title":"Bio Med Central"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0214332"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2011.0118"},{"key":"e_1_2_10_30_2","article-title":"AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading","volume":"31","author":"Trott O.","year":"2009","journal-title":"Journal of Computational Chemistry"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12944-016-0393-2"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fob.2014.08.003"},{"key":"e_1_2_10_33_2","article-title":"The lipid-lowering effect of quercetin on hyperlipidemia rats","volume":"8","author":"Cheng B.","year":"2011","journal-title":"The Chinese and foreign health abstract"},{"key":"e_1_2_10_34_2","article-title":"Study on the effect of quercetin on experimental hypertension, hyperglycemia and hyperlipidemia in rats","volume":"11","author":"Zhai Y. P.","year":"2012","journal-title":"Journal of Xuzhou Medical University"},{"key":"e_1_2_10_35_2","first-page":"169","article-title":"Protective effect of isorhamnetin on H2O2-induced injury of human umbilical vein endothelial cells","volume":"17","author":"Zhang F.","year":"2011","journal-title":"Chinese Journal of Experimental Traditional Medical Formulae"},{"key":"e_1_2_10_36_2","first-page":"853","article-title":"Study on the protective effect of isorhamnetin on H2O2 induced oxidative stress injury of H9C2 cells","volume":"31","author":"Dong X.","year":"2015","journal-title":"Chinese Pharmacological Bulletin"},{"key":"e_1_2_10_37_2","doi-asserted-by":"publisher","DOI":"10.1134\/S0006297909030092"},{"key":"e_1_2_10_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11373-005-9031-0"},{"key":"e_1_2_10_39_2","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.6383"},{"key":"e_1_2_10_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2013.11.013"},{"key":"e_1_2_10_41_2","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.27173"},{"key":"e_1_2_10_42_2","article-title":"Relationship between interleukin-6 gene polymorphism and coronary heart disease and its effect on plasma lipid levels","volume":"4","author":"Wei Y. S.","year":"2006","journal-title":"Chinese Critical Care Medicine"},{"key":"e_1_2_10_43_2","first-page":"398","article-title":"Effects of Xuezhikang on blood lipid and interleukin-6 in obese rats","volume":"27","author":"Sun D. S.","year":"2011","journal-title":"The Chinese Journal of Clinical Pharmacology"},{"key":"e_1_2_10_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4004(88)90076-8"},{"key":"e_1_2_10_45_2","first-page":"1086","article-title":"Experimental study on the expression of COX-2 mRNA in atherosclerosis rats and the intervention of reseratrol","volume":"29","author":"Zhang L.","year":"2009","journal-title":"Chinese Journal of Gerontology"},{"key":"e_1_2_10_46_2","article-title":"The significance of cyclooxygenase 2 in atherosclerotic inflammation and the influence of statins","volume":"2","author":"Deng P.","year":"2004","journal-title":"Chinese Journal of Arteriosclerosis"},{"key":"e_1_2_10_47_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000140720.79015.3C"},{"key":"e_1_2_10_48_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/2151302"},{"key":"e_1_2_10_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2004.05.001"},{"key":"e_1_2_10_50_2","doi-asserted-by":"publisher","DOI":"10.4161\/23723556.2014.980184"},{"key":"e_1_2_10_51_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells8030214"},{"key":"e_1_2_10_52_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00464"},{"key":"e_1_2_10_53_2","first-page":"446","article-title":"The relationship between blood flow shear stress signal transduction and atherosclerosis","volume":"28","author":"Wu C. Y.","year":"2009","journal-title":"Journal of Southeast University (Medical Science Edition)"},{"key":"e_1_2_10_54_2","first-page":"513","article-title":"Shear stress signal transduction mechanism of vascular endothelial cells and atherosclerosis","volume":"4","author":"Zhang Y.","year":"2005","journal-title":"Chinese Journal of Arteriosclerosis"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2021\/9965906.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2021\/9965906.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2021\/9965906","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T06:52:32Z","timestamp":1735627952000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2021\/9965906"}},"subtitle":[],"editor":[{"given":"Baojun","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":54,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1155\/2021\/9965906"],"URL":"https:\/\/doi.org\/10.1155\/2021\/9965906","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"2021-03-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"9965906"}}