{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T18:38:38Z","timestamp":1776451118151,"version":"3.51.2"},"reference-count":68,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,7,8]],"date-time":"2021-07-08T00:00:00Z","timestamp":1625702400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Behbahan Faculty of Medical Sciences, Behbahan, Iran","award":["99013"],"award-info":[{"award-number":["99013"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Parasitology Research"],"published-print":{"date-parts":[[2021,7,8]]},"abstract":"<jats:p>Apicomplexan parasites, including Toxoplasma gondii (T. gondii), express different types of calcium-dependent protein kinases (CDPKs), which perform a variety of functions, including attacking and exiting the host cells. In the current bioinformatics study, we have used several web servers to predict the basic features and specifications of the CDPK7 protein. The findings showed that CDPK7 protein has 2133 amino acid residues with an average molecular weight (MW) of 219085.79\u2009D. The aliphatic index with 68.78 and grand average of hydropathicity (GRAVY) with -0.331 score were estimated. The outcomes of current research showed that the CDPK7 protein included 502 alpha-helix, 1311 random coils, and 320 extended strands with GOR4 method. Considering the Ramachandran plot, the favored region contains more than 92% of the amino acid residues. In addition, evaluation of antigenicity and allergenicity showed that CDPK7 protein has immunogenic and nonallergenic nature. The present research provides key data for more animal-model study on the CDPK7 protein to design an efficient vaccine against toxoplasmosis in the future.<\/jats:p>","DOI":"10.1155\/2021\/9974509","type":"journal-article","created":{"date-parts":[[2021,7,12]],"date-time":"2021-07-12T20:50:53Z","timestamp":1626123053000},"page":"1-20","source":"Crossref","is-referenced-by-count":6,"title":["Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii Vaccine"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6876-5902","authenticated-orcid":true,"given":"Ali","family":"Taghipour","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3837-1961","authenticated-orcid":true,"given":"Sanaz","family":"Tavakoli","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4670-767X","authenticated-orcid":true,"given":"Mohamad","family":"Sabaghan","sequence":"additional","affiliation":[{"name":"Behbahan Faculty of Medical Sciences, Behbahan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8661-7217","authenticated-orcid":true,"given":"Masoud","family":"Foroutan","sequence":"additional","affiliation":[{"name":"USERN Office, Abadan University of Medical Sciences, Abadan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5568-1366","authenticated-orcid":true,"given":"Hamidreza","family":"Majidiani","sequence":"additional","affiliation":[{"name":"Zoonotic Diseases Research Center, Ilam University of Medical Sciences, Ilam, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9898-1297","authenticated-orcid":true,"given":"Shahrzad","family":"Soltani","sequence":"additional","affiliation":[{"name":"USERN Office, Abadan University of Medical Sciences, Abadan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1860-326X","authenticated-orcid":true,"given":"Milad","family":"Badri","sequence":"additional","affiliation":[{"name":"Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9635-2876","authenticated-orcid":true,"given":"Ali Dalir","family":"Ghaffari","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4434-756X","authenticated-orcid":true,"given":"Sheyda","family":"Soltani","sequence":"additional","affiliation":[{"name":"USERN Office, Abadan University of Medical Sciences, Abadan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2008.00345.x"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2019.04.012"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2009.01.005"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.fawpar.2019.e00049"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2016.03.002"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007807"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1093\/trstmh\/traa133"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2020.01.008"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00389"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-020-05396-5"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02407-7"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.jogoh.2017.12.003"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1590\/0037-8682-0251-2017"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2017.10.010"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.05.041"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1179\/2047773213Y.0000000094"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-008-0612-5"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.2015.1098539"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.17179\/excli2018-1993"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.7774\/cevr.2019.8.1.4"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.7774\/cevr.2018.7.2.93"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2021.105837"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2020.104488"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiolchem.2019.107158"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.7774\/cevr.2021.10.1.59"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2020.104386"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2020.104079"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.7774\/cevr.2020.9.2.81"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-021-05467-1"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2021.105836"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2020.101413"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2018.04.033"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2007142141"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.7774\/cevr.2018.7.1.24"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12186"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.4161\/hv.26687"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-890-0:571"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1515\/ap-2016-0042"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.7774\/cevr.2020.9.2.146"},{"key":"40","first-page":"540","article-title":"GOR method for predicting protein secondary structure from amino acid sequence","volume-title":"Methods Enzymol","author":"J. Garnier","year":"1996"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200900140"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1002\/prot.24086"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-09654-8"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm290"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-30220-9_16"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1002\/prot.21078"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1021\/bi00367a013"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1186\/1745-7580-2-2"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(90)80535-Q"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.55.3.836-839.1985"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1002\/9780470122921.ch2"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1007\/BF01195768"},{"key":"53","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-9-514"},{"key":"54","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv639"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1000048"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.02.005"},{"key":"57","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-8-4"},{"key":"58","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt619"},{"key":"59","first-page":"S4","article-title":"AllerTOP-a server for in silico prediction of allergens","volume-title":"BMC bioinformatics","author":"I. Dimitrov","year":"2013"},{"key":"60","doi-asserted-by":"publisher","DOI":"10.1016\/j.fawpar.2019.e00037"},{"key":"61","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2018.11.003"},{"key":"62","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2020.03.006"},{"key":"63","first-page":"595","article-title":"Antigen-antibody interaction","volume-title":"Fundamental immunology","author":"J. Berzofsky","year":"1984"},{"key":"64","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-2-111"},{"key":"65","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-015-4824-1"},{"issue":"1","key":"66","first-page":"17","article-title":"T-cell immunity in human chronic toxoplasmosis","volume":"41","author":"I. El-Kady","year":"2011","journal-title":"Journal of the Egyptian Society of Parasitology"},{"key":"67","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1026-1033.2000"},{"key":"68","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2011.10.024"}],"container-title":["Journal of Parasitology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jpr\/2021\/9974509.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jpr\/2021\/9974509.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jpr\/2021\/9974509.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,12]],"date-time":"2021-07-12T20:51:05Z","timestamp":1626123065000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jpr\/2021\/9974509\/"}},"subtitle":[],"editor":[{"given":"Jos\u00e9 F.","family":"Silveira","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,7,8]]},"references-count":68,"alternative-id":["9974509","9974509"],"URL":"https:\/\/doi.org\/10.1155\/2021\/9974509","relation":{},"ISSN":["2090-0031","2090-0023"],"issn-type":[{"value":"2090-0031","type":"electronic"},{"value":"2090-0023","type":"print"}],"subject":[],"published":{"date-parts":[[2021,7,8]]}}}