{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T14:01:03Z","timestamp":1782828063797,"version":"3.54.5"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T00:00:00Z","timestamp":1624838400000},"content-version":"vor","delay-in-days":178,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p><jats:italic>Nigella sativa<\/jats:italic> (NS) is a well\u2010known plant for its various benefits and multiuse in traditional medicine. This study is aimed at investigating the chemical composition of the different <jats:italic>NS<\/jats:italic> fractions by using GC\u2010MS for the esterified fatty acids or HPLC\u2010UV for organic fraction and at evaluating the inhibitory effect on pancreatic <jats:italic>\u03b1<\/jats:italic>\u2010amylase (<jats:italic>in vitro<\/jats:italic>, <jats:italic>in vivo<\/jats:italic>) and intestinal glucose absorption. Among all the investigated fractions, it was shown that they are rich with different molecules of great interest. The n\u2010hexane fraction was characterized by the presence of linoleic acid (44.65%), palmitic acid (16.32%), stearic acid (14.60%), and thymoquinone (8.7%), while among the identified peaks in EtOH fraction we found catechin (89.03\u2009mg\/100\u2009g DW), rutin (6.46\u2009mg\/100\u2009g DW), and kaempferol (0.032\u2009mg\/100\u2009g DW). The MeOH fraction was distinguished with the presence of gallic acid (19.91\u2009mg\/100\u2009g DW), catechin (13.79\u2009mg\/100\u2009g DW), and rutin (21.07\u2009mg\/100\u2009g DW). Finally, the aqueous fraction was marked by the existence of different molecules; among them, we mention salicylic acid (32.26\u2009mg\/100\u2009g DW), rutin (21.46\u2009mg\/100\u2009g DW), and vanillic acid (3.81\u2009mg\/100\u2009g DW). Concerning the inhibitory effect on pancreatic <jats:italic>\u03b1<\/jats:italic>\u2010amylase, it was found that in the <jats:italic>in vitro<\/jats:italic> study, the best IC<jats:sub>50<\/jats:sub> registered were those of EtOH (0.25\u2009mg\/ml), MeOH (0.10\u2009mg\/ml), aqueous (0.031\u2009mg\/ml), and n\u2010hexane fraction (0.76\u2009mg\/ml), while in the <jats:italic>in vivo<\/jats:italic> study an important inhibition of <jats:italic>\u03b1<\/jats:italic>\u2010amylase in normal and diabetic rats was observed. Finally, the percentage of intestinal glucose absorption was evaluated for all tested extracts and it was ranging from 24.82 to 60.12%. The results of the present study showed that the <jats:italic>NS<\/jats:italic> seed fractions exert an interesting inhibitory effect of <jats:italic>\u03b1<\/jats:italic>\u2010amylase and intestinal glucose absorption activity which could be associated with the existent bioactive compounds. Indeed, these compounds can be used as antidiabetic agents because of their nontoxic effect and high efficacy.<\/jats:p>","DOI":"10.1155\/2021\/9979419","type":"journal-article","created":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T19:20:32Z","timestamp":1624908032000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Chemical Composition Analysis Using HPLC\u2010UV\/GC\u2010MS and Inhibitory Activity of Different <i>Nigella sativa<\/i> Fractions on Pancreatic <i>\u03b1<\/i>\u2010Amylase and Intestinal Glucose Absorption"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0722-7414","authenticated-orcid":false,"given":"Mohammed","family":"Dalli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3516-318X","authenticated-orcid":false,"given":"Nour Elhouda","family":"Daoudi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9991-8924","authenticated-orcid":false,"given":"Salah-eddine","family":"Azizi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hind","family":"Benouda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9473-1290","authenticated-orcid":false,"given":"Mohamed","family":"Bnouham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Gseyra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,28]]},"reference":[{"key":"e_1_2_9_1_2","volume-title":"Diabetes Mellitus","author":"Raffel L. 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