{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:50:57Z","timestamp":1790859057999,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T00:00:00Z","timestamp":1640649600000},"content-version":"vor","delay-in-days":361,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. The pathogenesis of long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) are well studied in psoriasis. However, little is known about how specific lncRNAs and miRNAs affect the mechanism of psoriasis development and which pathways are involved. <jats:italic>Objectives<\/jats:italic>. To explore the role of the lncRNA H19\/miR\u2010766\u20103p\/S1PR3 axis in psoriasis. <jats:italic>Methods<\/jats:italic>. miRNA and lncRNA microarrays were performed using IL\u201022\u2010induced HaCaT cells and psoriatic lesions, respectively. Fluorescence <jats:italic>in situ<\/jats:italic> hybridization and quantitative reverse\u2010transcriptase polymerase chain reaction were used to detect the expression of miR\u2010766\u20103p and lncRNA H19. Luciferase reporter assays were used to identify miR\u2010766\u20103p\/lncRNA H19 and miR\u2010766\u20103p\/S1PR3 combinations. CCK\u20108 and ELISA were performed to evaluate the proliferation of keratinocytes and the secretion of pro\u2010inflammatory cytokines. Western blot analysis was used to detect the expression of S1PR3 and its downstream effector proteins. <jats:italic>Results<\/jats:italic>. MiR\u2010766\u20103p was upregulated in both HaCaT cells treated with the psoriasis\u2010related cytokine pool (IL\u201017A, IL\u201022, IL\u20101 alpha, oncostatin M, and TNF\u2010alpha) and tissues. Overexpression of miR\u2010766\u20103p promoted keratinocyte proliferation and IL\u201017A and IL\u201022 secretion. LncRNA H19 and S1PR3 were demonstrably combined with miR\u2010766\u20103p by luciferase reporter assay. lncRNA H19 repressed proliferation and inflammation, which were reduced by the miR\u2010766\u20103p. AKT\/mTOR pathway effected proliferation and inflammation by the lncRNA H19\/miR\u2010766\u20103p\/S1PR3 axis. <jats:italic>Conclusions<\/jats:italic>. We established that downregulation of lncRNA H19 promoted the proliferation of keratinocytes and skin inflammation by up\u2010regulating miR\u2010766\u20103p expression levels and inhibiting activation of S1PR3 through the AKT\/mTOR pathway in psoriasis.<\/jats:p>","DOI":"10.1155\/2021\/9991175","type":"journal-article","created":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T15:50:26Z","timestamp":1640706626000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["The lncRNA H19\/miR\u2010766\u20103p\/S1PR3 Axis Contributes to the Hyperproliferation of Keratinocytes and Skin Inflammation in Psoriasis via the AKT\/mTOR Pathway"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5702-1393","authenticated-orcid":false,"given":"Yuexi","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiran","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianjun","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3565-3530","authenticated-orcid":false,"given":"Qing","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,12,28]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)61909-7"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.4006"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rdc.2015.07.013"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-15-3449-2_8"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2521"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.02.012"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2019.00263"},{"key":"e_1_2_8_8_2","first-page":"322","article-title":"LncRNA H19 promotes atherosclerosis by regulating MAPK and NF-kB signaling pathway","volume":"21","author":"Pan J. 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