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Dual-positive Th2\u2013Th17 cells, as newly discovered T-helper cells, have the functions of Th2 and Th17 cells and can coproduce Th2 and Th17 cytokines. Previous studies have shown that dual-positive Th2\u2013Th17 cells increase the chances of asthma and correlate with asthma severity. However, the exact role of dual-positive Th2\u2013Th17 cells in asthma is not known. Since there is no mature differentiation method for dual-positive Th2\u2013Th17 cells, the present study aimed to clarify the strict differentiation conditions and reveal how dual-positive Th2\u2013Th17 cells regulate asthma phenotypes. In this study, we confirmed that IL-1\u03b2, IL-6, anti-IFN-\u03b3, and IL-21 promoted biphenotypic cell differentiation. Moreover, more proportion of dual-positive Th2\u2013Th17 cells can be obtained by conditioned differentiation of mouse CD4+ T cells after classical allergic asthma modeling. Before asthma modeling, adoptive dual-positive Th2\u2013Th17 cells promoted T cells to differentiate into the same biphenotype cells and exacerbated the severity of asthma. Together, our results clarify the differentiation conditions of dual-positive Th2\u2013Th17 cells and further confirm that it stimulates asthma T cells to differentiate into the same biphenotype cells, leading to exacerbation of asthma.<\/jats:p>","DOI":"10.1155\/2021\/9999122","type":"journal-article","created":{"date-parts":[[2021,8,6]],"date-time":"2021-08-06T18:20:37Z","timestamp":1628274037000},"page":"1-8","source":"Crossref","is-referenced-by-count":9,"title":["Increased Proportion of Dual-Positive Th2\u2013Th17 Cells Promotes a More Severe Subtype of Asthma"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1124-1550","authenticated-orcid":true,"given":"Wenjin","family":"Sun","sequence":"first","affiliation":[{"name":"Department of General Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China"},{"name":"Lab of Lung Immuno-Inflammation, Frontiers Science Center for Disease-Related Molecular Network, Sichuan University, Chengdu, Sichuan 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lulu","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingping","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingsi","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4707-7809","authenticated-orcid":true,"given":"Xudong","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Emergency, The Second Xiangya Hospital of Central South University, Emergency and Difficult Diseases Institute of Central South University, Changsha, Hunan 410011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aijun","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, The Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, China"},{"name":"No. 3 Emergency Department of Yuelushan Hospital District, Hunan Provincial People\u2019s Hospital, Changsha 410000, Hunan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Libing","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, The Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9839-2342","authenticated-orcid":true,"given":"Shaokun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6572-5641","authenticated-orcid":true,"given":"Bing","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Emergency, The Second Xiangya Hospital of Central South University, Emergency and Difficult Diseases Institute of Central South University, Changsha, Hunan 410011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(13)61536-6"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00853-2015"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2678"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1111\/all.12828"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1038\/nri2870"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2016.10.038"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2018.08.010"},{"issue":"8","key":"8","doi-asserted-by":"crossref","first-page":"3779","DOI":"10.4049\/jimmunol.154.8.3779","article-title":"Induction of a Th2 population from a polarized leishmania-specific Th1 population by in vitro culture with IL-4","volume":"154","author":"S. 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