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Anthrax toxin receptor 1 (ANTXR1) is a type I transmembrane protein that binds the anthrax toxin ligands and mediates the entry of its toxic part into cells. It also functions as a receptor for the Protective antigen (PA) of anthrax toxin, and mediates the entry of Edema factor (EF) and Lethal factor (LF) into the cytoplasm of target cells and exerts their toxicity. Previous research has shown that ANTXR1 inhibits the expression of \u03b3-globin during the differentiation of erythroid cells. However, the effect on erythropoiesis from a cellular perspective has not been fully determined. This study examined the role of ANTXR1 on erythropoiesis using K562 and HUDEP-2 cell lines as well as cord blood CD34+ cells. Our study has shown that overexpression of ANTXR1 can positively regulate erythrocyte proliferation, as well as inhibit GATA1 and ALAS2 expression, differentiation, and apoptosis in K562 cells and hematopoietic stem cells. ANTXR1 knockdown inhibited proliferation, promoted GATA1 and ALAS2 expression, accelerated erythrocyte differentiation and apoptosis, and promoted erythrocyte maturation. Our study also showed that ANTXR1 may regulate the proliferation and differentiation of hematopoietic cells, though the Wnt\/\u03b2-catenin pathway, which may help to establish a possible therapeutic target for the treatment of blood disorders.<\/jats:p>","DOI":"10.1155\/2022\/1226697","type":"journal-article","created":{"date-parts":[[2022,8,27]],"date-time":"2022-08-27T15:20:08Z","timestamp":1661613608000},"page":"1-15","source":"Crossref","is-referenced-by-count":2,"title":["ANTXR1 Regulates Erythroid Cell Proliferation and Differentiation through wnt\/\u03b2-Catenin Signaling Pathway In Vitro and in Hematopoietic Stem Cell"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9468-069X","authenticated-orcid":true,"given":"Tingting","family":"Jin","sequence":"first","affiliation":[{"name":"School of Medicine, Guizhou University, Guiyang, Guizhou 550025, China"},{"name":"Prenatal Diagnosis Center, Guizhou Provincial People\u2019s Hospital, Guiyang, Guizhou 550002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaojun","family":"Zhang","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Han","sequence":"additional","affiliation":[{"name":"School of Medicine, Guizhou University, Guiyang, Guizhou 550025, China"},{"name":"Prenatal Diagnosis Center, Guizhou Provincial People\u2019s Hospital, Guiyang, Guizhou 550002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Li","sequence":"additional","affiliation":[{"name":"Prenatal Diagnosis Center, Guizhou Provincial People\u2019s Hospital, Guiyang, Guizhou 550002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Medicine, Guizhou University, Guiyang, Guizhou 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minmin","family":"Jiang","sequence":"additional","affiliation":[{"name":"Prenatal Diagnosis Center, Guizhou Provincial People\u2019s Hospital, Guiyang, Guizhou 550002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryo","family":"Kurita","sequence":"additional","affiliation":[{"name":"Cell Engineering Division, RIKEN Bio Resource Center, Tsukuba, Ibaraki 305-0074, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukio","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Cell Engineering Division, RIKEN Bio Resource Center, Tsukuba, Ibaraki 305-0074, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangfang","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Laboratory, Guizhou Provincial People\u2019s Hospital, Guiyang, Guizhou 550002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6628-8620","authenticated-orcid":true,"given":"Xiangdong","family":"Fang","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0394-240X","authenticated-orcid":true,"given":"Shengwen","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Medicine, Guizhou University, Guiyang, Guizhou 550025, China"},{"name":"Prenatal Diagnosis Center, Guizhou Provincial People\u2019s Hospital, Guiyang, Guizhou 550002, China"},{"name":"NHC Key Laboratory of Pulmonary Immunological Diseases, Guizhou Provincial People\u2019s Hospital, Guiyang, Guizhou 550002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6483-734X","authenticated-orcid":true,"given":"Zhaolin","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Medicine, Guizhou University, Guiyang, Guizhou 550025, China"},{"name":"Department of Urology, Guizhou Provincial People\u2019s Hospital, Guiyang, Guizhou 550002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0076-6879(06)19007-2","article-title":"Hematopoietic stem cells","volume":"419","author":"R. 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