{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T17:25:21Z","timestamp":1740158721708,"version":"3.37.3"},"reference-count":29,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,2,12]],"date-time":"2022-02-12T00:00:00Z","timestamp":1644624000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100019446","name":"Medical and Health Science and Technology Development Project of Shandong Province","doi-asserted-by":"publisher","award":["2019WS412"],"award-info":[{"award-number":["2019WS412"]}],"id":[{"id":"10.13039\/501100019446","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Healthcare Engineering"],"published-print":{"date-parts":[[2022,2,12]]},"abstract":"<jats:p>Background. Abundant reports have uncovered an imbalance of Treg and Th17 cells in pulmonary diseases. Hereon, we intend to explore the impact of PARP-1 on the imbalance of Th17\/Treg and the potential mechanism in premature rats with acute respiratory distress syndrome (ARDS). Methods. Preterm ARDS infants and healthy term infants were enrolled in this investigation. To induce a rat model of ARDS, E.coli suspension was given to rats through two vaginal dilator-guided intramuscular injections. H&amp;E staining was used to perform histopathological examination. Flow cytometry was employed to assess the proportion of Th17 or Treg cells accounted for CD4+ T cells. ELISA was applied to measure levels of IL-6, IL-17A, and IL-10 in the serum of ARDS patients. Moreover, the mRNA and protein expression levels of PARP-1, IL-6, IL-17A, and IL-10 were detected through qRT-PCR and western blotting. Results. An increased Th17\/Treg ratio was observed in preterm infants and rats with ARDS. The PARP-1 expression level was raised in the lung tissues of ARDS rats, and PARP-1 downregulation alleviated E.coli-induced lung injury in preterm rats. Expression levels of PARP-1, IL-6, and IL-17A were raised, and the IL-10 level was reduced in the lung tissues of rats after E.coli treatment, which was all reversed by PARP-1 suppression. Importantly, the ratio of Th17\/Treg differentiated from purified CD4+ T cells of the E.coli\u2009+\u2009PARP-1 inhibitor group was elevated by recombinant IL-6. Conclusion. PARP-1 downregulation repressed the imbalance of Th17 and Treg cells via reducing the expression level of IL-6, implying that PARP-1 may be a promising target for ARDS therapy.<\/jats:p>","DOI":"10.1155\/2022\/1255674","type":"journal-article","created":{"date-parts":[[2022,2,12]],"date-time":"2022-02-12T20:20:12Z","timestamp":1644697212000},"page":"1-9","source":"Crossref","is-referenced-by-count":1,"title":["PARP-1 Inhibition Repressed Imbalance of Th17 and Treg Cells in Preterm Rats with Intrauterine Infection-Induced Acute Respiratory Distress Syndrome by Reducing the Expression Level of IL-6"],"prefix":"10.1155","volume":"2022","author":[{"given":"Qian","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Shandong Provincial Maternal and Child Health Care Hospital, No 238, Jingshi Eastern Road, Jinan 250014, Shandong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ensheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Shandong Provincial Maternal and Child Health Care Hospital, No 238, Jingshi Eastern Road, Jinan 250014, Shandong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Guangdong General Hospital, No 106, Zhongshan Two Road, Guangzhou 510080, Guangdong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peipei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Shandong Provincial Maternal and Child Health Care Hospital, No 238, Jingshi Eastern Road, Jinan 250014, Shandong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5899-4429","authenticated-orcid":true,"given":"Lei","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Shandong Provincial Maternal and Child Health Care Hospital, No 238, Jingshi Eastern Road, Jinan 250014, Shandong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1183\/16000617.0116-2016"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1056\/nejmra1608077"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.4046\/trd.2016.79.3.188"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2016.00060"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(08)60074-4"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-018-0787-y"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2018.01.009"},{"issue":"1","key":"8","first-page":"383","article-title":"Changes in TNF-alpha, IL-6, IL-10 and VEGF in rats with ARDS and the effects of dexamethasone","volume":"17","author":"M. 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