{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:35:51Z","timestamp":1787841351205,"version":"build-2784847793"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,7,9]],"date-time":"2022-07-09T00:00:00Z","timestamp":1657324800000},"content-version":"vor","delay-in-days":189,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009377","name":"Education Department of Hunan Province","doi-asserted-by":"publisher","award":["18C1853"],"award-info":[{"award-number":["18C1853"]}],"id":[{"id":"10.13039\/100009377","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2020JJ5384"],"award-info":[{"award-number":["2020JJ5384"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>Ischemic stroke is a common disease that led to high mortality and high disability. NADPH oxidase 2\u2010 (NOX2\u2010) mediated oxidative stress and long noncoding RNA have important roles in cerebral ischemia\/reperfusion (CI\/R) injury, whereas whether there is interplay between them remains to be clarified. This study was performed to observe the role of lncRNA PINK1\u2010antisense RNA (PINK1\u2010AS) in NOX2 expression regulation. An in vivo rat model (MCAO) and an in vitro cell model (H\/R: hypoxia\/reoxygenation) were utilized for CI\/R oxidative stress injury investigation. The expression levels of lncRNA PINK1\u2010AS, activating transcription factor 2 (ATF2), NOX2, and caspase\u20103 and the production level of ROS and cell apoptosis were significantly increased in CI\/R injury model rats or in H\/R\u2010induced SH\u2010SY5Y cells, but miR\u2010203 was significantly downregulated. There was positive correlation between PINK1\u2010AS expression level and ROS production level. PINK1\u2010AS and ATF2 were found to be putative targets of miR\u2010203. Knockdown of lncRNA PINK1\u2010AS or ATF2 or the overexpression of miR\u2010203 significantly reduced oxidative stress injury via inhibition of NOX2. Overexpression of lncRNA PINK1 significantly led to oxidative stress injury in SH\u2010SY5Y cells through downregulating miR\u2010203 and upregulating ATF2 and NOX2. lncRNA PINK1\u2010AS and ATF2 were the targets of miR\u2010203, and the lncRNA PINK1\u2010AS\/miR\u2010203\/ATF2\/NOX2 axis plays pivotal roles in CI\/R injury. Therefore, lncRNA PINK1\u2010AS is a possible target for CR\/I injury therapy by sponging miR\u2010203.<\/jats:p>","DOI":"10.1155\/2022\/1296816","type":"journal-article","created":{"date-parts":[[2022,7,9]],"date-time":"2022-07-09T18:05:09Z","timestamp":1657389909000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["lncRNA PINK1\u2010AS Aggravates Cerebral Ischemia\/Reperfusion Oxidative Stress Injury through Regulating ATF2 by Sponging 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