{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T04:16:49Z","timestamp":1791605809420,"version":"4.3.4"},"reference-count":30,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T00:00:00Z","timestamp":1654214400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81800548"],"award-info":[{"award-number":["81800548"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cardiovascular Therapeutics"],"published-print":{"date-parts":[[2022,6,3]]},"abstract":"<jats:p>Background. Inflammation is a critical factor in the development and progression of myocardial infarction and cardiac fibrosis. Thymosin \u03b24 (T\u03b24) alleviates the disease process via protective antioxidant and anti-inflammatory mechanisms. Although T\u03b24 has been shown to have a protective effect in myocardial infarction, its impact on cardiac fibrosis has not been well reported. In this study, we evaluated the influence of exogenous T\u03b24 on myocardial infarction and cardiac fibrosis and explored the possible underlying mechanism. Methods. Real-time quantitative reverse-transcription PCR (qRT-PCR), immunohistochemistry (IHC), and Western blot were used to analyze T\u03b24 expression in acute myocardial infarction (AMI) cardiac tissues. The effects of intraperitoneal adeno-associated virus-T\u03b24 (AAV-T\u03b24) on ligation-induced AMI in mice were studied using cardiac function parameters, and RT-PCR, Western blot, HE staining, Masson staining, and IHC were used to assess the degree of myocardial fibrosis. The effects of T\u03b24 were confirmed in vitro using mouse cardiac myocytes and myofibroblasts. Results. T\u03b24 was shown to be significantly elevated in mice AMI cardiac tissues. In mice, AAV-T\u03b24 induced exogenous expression of T\u03b24 significantly reduced oxidative damage, inflammation, cardiac dysfunction, and fibrosis. H2O2 inhibited mitophagy and increased inflammation in mouse cardiac myocytes via oxidative stress, and T\u03b24 substantially reduced mitophagy inhibition and inflammasome activation in myocytes caused by H2O2. Furthermore, T\u03b24 decreased cardiac myofibroblast growth and reduced TGF-\u03b21-induced activation. Conclusions. AAV-T\u03b24 induced expression of T\u03b24 reduced inflammation, heart damage, and eventual fibrosis in vivo. T\u03b24 helped to reduce oxidative stress, promote mitophagy, and alleviate inflammation and fibrosis. Exogenous supplementation of T\u03b24 might be a promising therapeutic agent for treating myocardial infarction as well as cardiac fibrosis.<\/jats:p>","DOI":"10.1155\/2022\/1308651","type":"journal-article","created":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T12:50:10Z","timestamp":1654260610000},"page":"1-13","source":"Crossref","is-referenced-by-count":12,"title":["Thymosin \u03b24 Protects against Cardiac Damage and Subsequent Cardiac Fibrosis in Mice with Myocardial Infarction"],"prefix":"10.1155","volume":"2022","author":[{"given":"Fei","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Ultrasound, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an, Shaanxi Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajuan","family":"He","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an, Shaanxi Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naijuan","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an, Shaanxi Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Litao","family":"Ruan","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an, Shaanxi Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7574-407X","authenticated-orcid":true,"given":"Zhen","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an, Shaanxi Province, China"},{"name":"Department of Infectious Diseases, The First Affiliated Hospital of Xi\u2019an Jiaotong University, 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