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In order to find some novel ways to inhibit biofilm formation, this study describe a previously unrecognized role for the human umbilical cord mesenchymal stem cells (hUCMSCs). In addition to multiple differentiation, hUCMSCs have the ability to prevent the biofilms formation in vitro by secreting antibacterial peptides (LL\u201037 and hBD\u20102). This occurred while <jats:italic>P. aeruginosa<\/jats:italic> PA27853 and hUCMSCs were cocultured, and the filtrated medium, which was the supernatant containing antibacterial peptides (5.9\u2009ng\/ml of LL\u201037, 1.77\u2009ng\/ml of hBD\u20102), and inhibited the growth of the bacterial biofilm on the surface of tracheal tube (2.5#, for preterm infant). Using microarrays, we were able to demonstrate that the antibacterial peptides from hUCMSC affected biofilm formation by downregulating the gene\u2010encoded polysaccharide biosynthesis protein. In addition, in order to find out the most suitable concentration of hUCMSCs, <jats:italic>P. aeruginosa<\/jats:italic> was cocultured with eight\u2010level concentrations of hUCMSCs, and we found that the concentration of LL\u201037 was positively correlated with the concentration of hUCMSCs. Meanwhile, the concentration of LL\u201037 became stable while the hUCMSC concentration reaches higher than 5 \u00d7 10<jats:sup>6<\/jats:sup>\u2009cells\/ml. But the concentration of hBD\u20102 had no significant correlation with hUCMSCs. The collection of these stem cells is not only limited by ethics but also reduces host rejection. This makes it possible to use autologous hUCMSCs to treat neonatal VAP.<\/jats:p>","DOI":"10.1155\/2022\/1530525","type":"journal-article","created":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T03:50:09Z","timestamp":1646365809000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Human Umbilical Cord Mesenchymal Stem Cells Prevent Bacterial Biofilm 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