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Human microvascular endothelial cells (hBMEC) and human normal astrocytes (HA-1800) were used to establish in vitro cocultured cell models. The coculture model was used to simulate hypoxic-ischemic stroke, and it was found that astrocytes could promote hBMEC proliferation, inhibit apoptosis, reduce cell damage, and enhance antioxidant capacity by activating the VEGF signaling pathway. When VEGF is knocked out in astrocytes, the protective effect of astrocytes on hBMEC was partially lost. In conclusion, our study confirms the protective effect of hBMEC and laid a foundation for the study of hypoxic-ischemic stroke.<\/jats:p>","DOI":"10.1155\/2022\/1884959","type":"journal-article","created":{"date-parts":[[2022,3,18]],"date-time":"2022-03-18T21:35:12Z","timestamp":1647639312000},"page":"1-8","source":"Crossref","is-referenced-by-count":2,"title":["Astrocytes Protect Human Brain Microvascular Endothelial Cells from Hypoxia Injury by Regulating VEGF Expression"],"prefix":"10.1155","volume":"2022","author":[{"given":"Jianmin","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1745-5496","authenticated-orcid":true,"given":"Jiwen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Zhangqiu District People\u2019s Hospital, Jinan, Shandong 250200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/853737"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.7150\/thno.29435"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1007\/s40119-018-0106-1"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S56071"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1186\/s13148-018-0549-1"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-11-66"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.2147\/cia.s223595"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1186\/s13041-016-0193-7"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1111\/jphp.12803"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1046\/j.1087-0024.2000.00003.x"},{"key":"11","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.1002\/(SICI)1522-2683(20000401)21:6<1082::AID-ELPS1082>3.0.CO;2-E","article-title":"Better approaches to fineding the needle in a haystack:Optimizing Proteome analysis through automation","volume":"21","author":"F. 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