{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:02:59Z","timestamp":1788566579515,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T00:00:00Z","timestamp":1648598400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007119","name":"Yasuj University of Medical Sciences","doi-asserted-by":"publisher","award":["960577"],"award-info":[{"award-number":["960577"]}],"id":[{"id":"10.13039\/501100007119","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Toxicology"],"published-print":{"date-parts":[[2022,3,30]]},"abstract":"<jats:p>Herein, the effects of hydroalcoholic extracts of Thymus daenensis Celak (TDC) and Stachys pilifera Benth (SPB) plants on HepG2 cell line were investigated by using different analyses. Cytotoxicity and apoptosis of extracts were investigated by MTT method, AnnV\/PI apoptosis assay, and their antioxidant capacity was evaluated by total thiol and glutathione peroxidase (GPX) assay. The results revealed that the SBP extract was more cytotoxic compared with the TDC extract and increased over time (128.49 \u00b5g\/mL vs 107.11 \u00b5g\/mL IC50 values for 24 and 72\u2009h, respectively). Although, AnnV\/PI apoptosis assay showed apoptosis induction for both extracts, but the caspase-3 activity assay revealed that TDC extract significantly increased caspase-3 activity compared with the control and SPB extract. Increasing the activity of GPX by SPB extract revealed that it has high antioxidant capacity. In conclusion, the TDC and SPB with high antioxidant capacity have high cytotoxicity against HepG2 cancer cells and have high capability as a medicinal plant.<\/jats:p>","DOI":"10.1155\/2022\/1924265","type":"journal-article","created":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T19:05:39Z","timestamp":1648667139000},"page":"1-8","source":"Crossref","is-referenced-by-count":17,"title":["Evaluation of the Antioxidant and Anticancer Activities of Hydroalcoholic Extracts of Thymus daenensis \u010celak and Stachys pilifera Benth"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1806-9888","authenticated-orcid":true,"given":"Zahra","family":"Barmoudeh","sequence":"first","affiliation":[{"name":"Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3526-9029","authenticated-orcid":true,"given":"Maryam Tajali","family":"Ardakani","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9166-419X","authenticated-orcid":true,"given":"Amir Hossein","family":"Doustimotlagh","sequence":"additional","affiliation":[{"name":"Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran"},{"name":"Department of Clinical Biochemistry, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8799-173X","authenticated-orcid":true,"given":"Hassan","family":"Bardania","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran"},{"name":"Clinical Research Development Unit, Imamsajad Hospital, Yasuj University of Medical Sciences, Yasuj, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1713263"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-108-3-390"},{"issue":"1","key":"3","first-page":"365","article-title":"A study on natural anticancer plants","volume":"1","author":"J Jena","year":"2012","journal-title":"Int J Pharmaceut Chem Sci"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.18203\/2394-6040.ijcmph20190060"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/7891574"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1903244116"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2003.50087"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-63032-5"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1002\/aoc.5394"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1080\/01635581.2019.1637006"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1080\/0972060x.2013.764190"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/s2095-4964(15)60166-2"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12034"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.17485\/ijst\/2012\/v5i1.3"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.eujim.2012.01.005"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.5812\/ircmj.19259"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.5897\/ajmr11.1066"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1080\/10412905.2009.9700123"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2009.06.037"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2019.e02072"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1111\/jfbc.13190"},{"key":"22","author":"M Stoddart"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1007\/s11239-016-1440-6"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1996.0292"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-020-05447-x"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/s0142-9612(02)00436-2"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.09.088"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12030562"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.11.055"},{"issue":"4","key":"30","first-page":"281","article-title":"Herbal medicine: current status and the future","volume":"4","author":"S. 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