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Many molecules like caspase-3, CD3, and CD138 are implicated in the tissue immune response in a trial to alleviate hazardous toxoplasmosis impact. This study aimed to investigate the effect of chronic toxoplasmosis on the liver and spleen tissues of mice using biochemical and histopathological techniques and to detect the activity and level of expression of caspase-3, CD3, and CD138 in these tissues using immunohistochemical labeling. Compared with normal control, altered normal histological features accompanied by inflammatory reaction were recorded in hepatosplenic reticuloendothelial tissues in chronically infected mice. The biochemical profile of the liver has been changed in the form of increased liver enzymes, and oxidative stress has been evidenced by elevated nitric oxide (NO) concentration in liver homogenate. The levels of caspase3, CD3, and CD138 were markedly expressed in the liver and spleen of infected mice. Our findings revealed the persistent effect of latent toxoplasmosis on the host\u2019s histological architecture, metabolic, and immunological profile, creating a continued challenging host-parasite relationship.<\/jats:p>","DOI":"10.1155\/2022\/2165205","type":"journal-article","created":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T00:50:32Z","timestamp":1655427032000},"page":"1-17","source":"Crossref","is-referenced-by-count":10,"title":["Histopathological, Immunohistochemical and Biochemical Studies of Murine Hepatosplenic Tissues Affected by Chronic Toxoplasmosis"],"prefix":"10.1155","volume":"2022","author":[{"given":"Samah Hassan","family":"Yahia","sequence":"first","affiliation":[{"name":"Medical Parasitology Department, Faculty of Medicine, Zagazig University, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samia Elsayed","family":"Etewa","sequence":"additional","affiliation":[{"name":"Medical Parasitology Department, Faculty of Medicine, Zagazig University, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nesreen Saeed","family":"Saleh","sequence":"additional","affiliation":[{"name":"Zoology Department, Faculty of Arts and Sciences Al-Wahat, Benghazi University, Libya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samira Metwally","family":"Mohammad","sequence":"additional","affiliation":[{"name":"Medical Parasitology Department, Faculty of Medicine, Zagazig University, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora Ibrahim","family":"Aboulfotouh","sequence":"additional","affiliation":[{"name":"Medical Parasitology Department, Faculty of Medicine, Mansoura University, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad Mansour","family":"Kandil","sequence":"additional","affiliation":[{"name":"Pathology Department, Faculty of Medicine, Al-Azhar University-Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5070-3922","authenticated-orcid":true,"given":"Mohamed Hassan","family":"Sarhan","sequence":"additional","affiliation":[{"name":"Medical Parasitology Department, Faculty of Medicine, Zagazig University, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.132.4.636"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.64.3.607-623.2000"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1086\/605433"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.2307\/3284282"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/s0020-7519(00)00124-7"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.apjtm.2015.09.003"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197905103001907"},{"issue":"10","key":"8","first-page":"1418","article-title":"Toxoplasmic chorioretinitis and hepatic granulomas","volume":"85","author":"T. 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