{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:19:52Z","timestamp":1767165592678,"version":"build-2238731810"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,1,17]],"date-time":"2022-01-17T00:00:00Z","timestamp":1642377600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Computational and Mathematical Methods in Medicine"],"published-print":{"date-parts":[[2022,1,17]]},"abstract":"<jats:p>Studies have demonstrated that miR-335-5p exhibits an essential role in the progress of multiple tumors, including thyroid cancer, pancreatic cancer, and non-small-cell lung cancer. However, the possible expression, the detailed role, and the underlying mechanisms of miR-335-5p in uterine leiomyoma (UL) still remained unclear. Therefore, the present study was designed to investigate the mechanism and function of miR-335-5p in UL. In our study, microRNA-335-5p (miR-335-5p) is significantly downregulated in UL tissues and UL cell lines, especially in HCC1688 and SK-UT-1 cells. Functionally, overexpression of miR-335-5p notably inhibits the viability of UL cell lines by CCK-8 assay. Besides, upregulation of miR-335-5p inhibits proliferation of UL cell lines by colony formation assay and decreases the protein levels of PCNA and Ki-67 detected by western blot assay. In addition, overexpression of miR-335-5p induces UL cell cycle arrest at G1 phase. Upregulation of miR-335-5p decreases the levels of Cyclin A1, Cyclin B1, and Cyclin D2 and upregulates the expression of p27 protein. Additionally, upregulation of miR-335-5p promotes the apoptosis of UL cell lines, increases the protein levels of Bax, Cleaved caspase-3, and Cleaved caspase-9, and decreases the protein expression of Bcl-2. Moreover, Arginine and Glutamate-Rich protein 1 (ARGLU1) is predicted as a target of miR-335-5p by ENCORI and miRDB and confirmed by dual-luciferase reporter assay. ARGLU1 is negatively associated with miR-335-5p. Furthermore, overexpression of ARGLU1 partly restores the effects of miR-335-5p mimic on the viability, proliferation, cell cycle, and apoptosis of UL cell lines. To conclude, miR-335-5p may play a repressive role in UL by targeting ARGLU1 and serve as a potential therapeutic target for the treatment of UL.<\/jats:p>","DOI":"10.1155\/2022\/2329576","type":"journal-article","created":{"date-parts":[[2022,1,17]],"date-time":"2022-01-17T16:05:26Z","timestamp":1642435526000},"page":"1-15","source":"Crossref","is-referenced-by-count":3,"title":["miR-335-5p Inhibits Progression of Uterine Leiomyoma by Targeting ARGLU1"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3048-5873","authenticated-orcid":true,"given":"Wei","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, 123 Tianfei Lane, Mochou Road, Nanjing, Jiangsu 210004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7063-3343","authenticated-orcid":true,"given":"Yingyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Zhangjiagang Hospital of Traditional Chinese Medicine & Affiliated Zhangjiagang Hospital of Nanjing University of Chinese Medicine, Zhangjiagang 215600, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8090-6766","authenticated-orcid":true,"given":"Ling","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Zhangjiagang Hospital of Traditional Chinese Medicine & Affiliated Zhangjiagang Hospital of Nanjing University of Chinese Medicine, Zhangjiagang 215600, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8491-590X","authenticated-orcid":true,"given":"Yan","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Zhangjiagang Hospital of Traditional Chinese Medicine & Affiliated Zhangjiagang Hospital of Nanjing University of Chinese Medicine, Zhangjiagang 215600, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7095-1299","authenticated-orcid":true,"given":"Sumei","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Geriatrics, The Affiliated Huai\u2019an Hospital of Xuzhou Medical University and the Second People\u2019s Hospital of Huai\u2019an, No. 62, Huaihai Road (S.), Huaian, Jiangsu 223002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/2414609"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmig.2019.04.021"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1159\/000347017"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.5787"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-018-1682-1"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2012.03.003"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19092524"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1177\/1933719118768692"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2019.12.016"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbs075"},{"key":"11","doi-asserted-by":"crossref","first-page":"4","DOI":"10.2174\/2211536607666180827111633","article-title":"MiRNAs: biology, biogenesis, their web-based tools, and databases","volume":"8","author":"M. 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