{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T14:05:26Z","timestamp":1751292326134,"version":"3.37.3"},"reference-count":56,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,9,13]],"date-time":"2022-09-13T00:00:00Z","timestamp":1663027200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013822","name":"Kurdistan University Of Medical Sciences","doi-asserted-by":"publisher","award":["IR.MUK.REC.1397\/211"],"award-info":[{"award-number":["IR.MUK.REC.1397\/211"]}],"id":[{"id":"10.13039\/501100013822","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2022,9,13]]},"abstract":"<jats:p>DNMT1, as a critical enzyme affecting epigenetics through methylation of DNA cytosine-rich sequences, regulates gene expression. Exterior factors including long-term infections, in this study Helicobacter pylori infection, could change host cells\u2019 epigenetics by affecting DNMT1 gene expression. This study investigated the statistical correlation between H. pylori virulence genes and DNMT1 gene expression in gastric antral epithelial cells of gastric adenocarcinoma and gastritis patients. In a case-control study, 50 and 53 gastritis and gastric adenocarcinoma antral biopsies, including 23 and 21 patients with H. pylori infection, respectively, were collected from hospitals in the west of Iran. Having extracted total RNA from gastric biopsy samples, cDNA was synthesized and virulence genes of H. pylori were detected by using the PCR method. Relative real-time RT PCR was used to detect \u0394\u0394Ct fold changes of the DNMT1 gene expression in divided groups of patients based on H. pylori infection and clinical manifestations. The results showed that along with increasing patients\u2019 age, the DNMT1 gene expression will increase in gastric antral epithelial cells of gastric cancer patients (<jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>P<\/a:mi>\n                        <a:mo>\u2264<\/a:mo>\n                        <a:mn>0.05<\/a:mn>\n                     <\/a:math>\n                  <\/jats:inline-formula>). On the other hand, the biopsy samples with infection of H. pylori cagA, cagY, and cagE genotypes revealed a direct correlation along with increased DNMT1 gene expression. This study revealed the correlations of H. pylori cag pathogenicity island genes with increased DNMT1 gene expression.<\/jats:p>","DOI":"10.1155\/2022\/2386891","type":"journal-article","created":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T00:20:14Z","timestamp":1663114814000},"page":"1-8","source":"Crossref","is-referenced-by-count":3,"title":["DNMT1 Gene Expression in Patients with Helicobacter pylori Infection"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0654-8918","authenticated-orcid":true,"given":"Manouchehr","family":"Ahmadi Hedayati","sequence":"first","affiliation":[{"name":"Liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran"},{"name":"Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9722-8826","authenticated-orcid":true,"given":"Amjad","family":"Ahmadi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran"},{"name":"Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3819-2074","authenticated-orcid":true,"given":"Zahed","family":"Khatooni","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Memorial University of Newfoundland, St Johns, NL, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12082123"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2017.3981"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/s0002-9440(10)63156-2"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-9967-2_1"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1186\/2045-3701-4-46"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2011.110"},{"issue":"10","key":"7","doi-asserted-by":"crossref","first-page":"1454","DOI":"10.1289\/ehp.10207","article-title":"Tumor suppressor gene inactivation during cadmium-induced malignant transformation of human prostate cells correlates with overexpression of de novo DNA methyltransferase","volume":"115","author":"L. 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