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J. Sm. has been extensively used in China, Japan, and Korea to treat osteoporosis and tonify kidneys. A rapid and validated UPLC\u2010MS\/MS method for simultaneous determination of the seven analytes including neoeriocitrin, luteolin\u20107\u2010<jats:italic>O-\u03b2<\/jats:italic>\u2010D\u2010glucoside, astragalin, naringin, eriodictyol, naringenin, and kaempferol in rats\u2019 various tissues (heart, liver, spleen, lung, kidney, stomach, brain, uterus, ovary, and small intestine) using quercetin as the internal standard (IS) was developed after oral administration of TFDR to rats. Tissues samples were retreated by protein precipitation with methanol. The chromatographic separation was performed using an ACQUITY UPLC\u2122 BEH C<jats:sub>18<\/jats:sub> column (2.1\u2009\u00d7\u200950\u2009mm; 1.7\u2009<jats:italic>\u03bc<\/jats:italic>m) at 35\u00b0C. The mobile phase consisted of 1% acetic acid in water as the aqueous phase (A) and 100% acetonitrile as the organic phase (B). All analytes and IS were quantified through electrospray ionization in positive ion multiple reaction monitoring (MRM) mode. MS transitions were m\/z 597.5\u2009\u27f6\u2009289.2 for neoeriocitrin, m\/z 449.1\u2009\u27f6\u2009287.1 for luteolin\u20107\u2010<jats:italic>O-\u03b2<\/jats:italic>\u2010D\u2010glucoside, m\/z 449.1\u2009\u27f6\u2009287.1 for astragalin, m\/z 581.5\u2009\u27f6\u2009273.2 for naringin, m\/z 289.2\u2009\u27f6\u2009153.1 for eriodictyol, m\/z 273.2\u2009\u27f6\u2009153.1 for naringenin, m\/z 287.1\u2009\u27f6\u2009153.1 for kaempferol, and m\/z 303.2\u2009\u27f6\u2009153.1 for quercetin (IS). The mean extraction recovery of the seven analytes and IS in tissue samples at three levels of quality control (QC) samples ranged from 82.72% to 118.57%, and the RSD was \u226414.98%. The intraday and interday precision (RSD) were all less than 14.98%, and the accuracy (RE) ranged from \u221213.96% to 14.96%, which indicated that the present method was not an issue. Tissues distribution showed neoeriocitrin, luteolin\u20107\u2010<jats:italic>O-\u03b2<\/jats:italic>\u2010D\u2010glucoside, astragalin, naringin, and naringenin could transfer across the blood\u2010brain barrier, which may form the basis of TFDR entering the brain to play an anti\u2010AD role. Compared with the 8\u2010month\u2010old rats, a higher concentration of naringin was found in the ovaries of the 18\u2010month\u2010old rats; this result indicated that it may regulate the autonomic nervous dysfunction of the cerebrospinal system through the hypothalamus\u2010pituitary\u2010ovary axis, thus playing an anti\u2010AD role, but further research is needed. Naringenin, eriodictyol, and kaempferol have a higher concentration in the liver and kidney; this phenomenon may be related to the traditional Chinese medicine theory that there is a definite relationship between the liver and kidney meridian. These results provide reliable data support for further study of the pharmacological mechanism of TFDR, formulation of drug delivery schemes, and development of new Chinese medicines in the treatment of AD.<\/jats:p>","DOI":"10.1155\/2022\/2447945","type":"journal-article","created":{"date-parts":[[2022,2,4]],"date-time":"2022-02-04T20:50:09Z","timestamp":1644007809000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Tissue Distribution of Total Flavonoids Extracts of <i>Drynariae Rhizoma<\/i> in Young and Old Rats by UPLC\u2013MS\/MS Determination"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3438-0163","authenticated-orcid":false,"given":"Yue","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2077-587X","authenticated-orcid":false,"given":"Xia","family":"Lei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongdan","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoliang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeqiu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huifeng","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4269-5421","authenticated-orcid":false,"given":"Fang","family":"Geng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0399-5085","authenticated-orcid":false,"given":"Ning","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,4]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1212\/con.0000000000000687"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2017.09.043"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0254-6272(13)60147-8"},{"key":"e_1_2_9_4_2","first-page":"1367","article-title":"Research thoughts and methods on concise prescriptions of Chinese medicine for prevention and treatment of Alzheimer\u2019s disease based on various kinds of animal models","volume":"14","author":"Liu M.","year":"2012","journal-title":"World Science and Technology (Modernization of Traditional Chinese Medicine and Materia Medica)"},{"key":"e_1_2_9_5_2","first-page":"60","article-title":"Discussion on researches about theory of \u2018kidney-Alzheimer\u2019s disease-stem cells\u2019","volume":"32","author":"Zhang Y. 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