{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T06:15:58Z","timestamp":1771654558826,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,2,14]],"date-time":"2022-02-14T00:00:00Z","timestamp":1644796800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Fund of China Youth Foundation Project","award":["81804207"],"award-info":[{"award-number":["81804207"]}]},{"name":"National Natural Science Fund of China Youth Foundation Project","award":["BSH2018015"],"award-info":[{"award-number":["BSH2018015"]}]},{"DOI":"10.13039\/501100008402","name":"Chengdu University of Traditional Chinese Medicine","doi-asserted-by":"publisher","award":["81804207"],"award-info":[{"award-number":["81804207"]}],"id":[{"id":"10.13039\/501100008402","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008402","name":"Chengdu University of Traditional Chinese Medicine","doi-asserted-by":"publisher","award":["BSH2018015"],"award-info":[{"award-number":["BSH2018015"]}],"id":[{"id":"10.13039\/501100008402","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2022,2,14]]},"abstract":"<jats:p>Objective. To determine whether electroacupuncture (EA) could alleviate visceral hypersensitivity in diarrhea-predominant irritable bowel syndrome (IBS-D) rats by inhibiting EGCs activity via the BDNF\/TrkB signaling pathway. Methods. Sprague Dawley rats were randomly divided to a control group (n\u2009=\u20098) and a model preparation group (n\u2009=\u200932), which received Senna solution by gavage and CUMS (chronic unpredictable mild stress) for 14 consecutive days and was further divided to a Model group, an EA group (only electroacupuncture), an EA\u2009+\u2009TrkB agonist group (electroacupuncture and TrkB), and an EA\u2009+\u2009DMSO group (electroacupuncture and DMSO, n\u2009=\u20098 for each). Rats in the three EA groups were acupunctured at ST25, ST36, and LR3 for 20\u2009min every day for 14 days. Abdominal withdrawal reflex (AWR) was used to quantify visceral sensitivity; reverse transcription polymerase chain reaction (RT-PCR) and double immunofluorescent staining were used to detect the colocalized expression of GFAP\/BDNF and GFAP\/TrkB. Western Blot (WB) was used to detect the expression of PLC and SP in the colon. Flow cytometry was used to detect the expression of Ca2+. Results. EA effectively alleviated visceral hypersensitivity in IBS-D rats (<jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>P<\/a:mi>\n                        <a:mo>&lt;<\/a:mo>\n                        <a:mn>0.05<\/a:mn>\n                     <\/a:math>\n                  <\/jats:inline-formula>). Compared to the control group, the expression of BDNF, TrkB, PLC, SP, and Ca2+ and the colocalized expression of GFAP\/BDNF and GFAP\/TrkB increased in the Model group (<jats:inline-formula>\n                     <c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                        <c:mi>P<\/c:mi>\n                        <c:mo>&lt;<\/c:mo>\n                        <c:mn>0.05<\/c:mn>\n                     <\/c:math>\n                  <\/jats:inline-formula>), while all these parameters decreased in the EA group following EA intervention (<jats:inline-formula>\n                     <e:math xmlns:e=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\">\n                        <e:mi>P<\/e:mi>\n                        <e:mo>&lt;<\/e:mo>\n                        <e:mn>0.05<\/e:mn>\n                     <\/e:math>\n                  <\/jats:inline-formula>). In addition, no significant difference was found between the EA\u2009+\u2009TrkB agonist group and the control group (<jats:inline-formula>\n                     <g:math xmlns:g=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\">\n                        <g:mi>P<\/g:mi>\n                        <g:mo>&gt;<\/g:mo>\n                        <g:mn>0.05<\/g:mn>\n                     <\/g:math>\n                  <\/jats:inline-formula>). Conclusions. EA alleviates visceral hypersensitivity of IBS-D rats possibly by inhibiting the activity of EGCs through the BDNF\/TrkB-PLC-Ca2+ signaling pathway in the colon.<\/jats:p>","DOI":"10.1155\/2022\/2497430","type":"journal-article","created":{"date-parts":[[2022,2,14]],"date-time":"2022-02-14T19:37:58Z","timestamp":1644867478000},"page":"1-11","source":"Crossref","is-referenced-by-count":8,"title":["Electroacupuncture Alleviates Visceral Hypersensitivity in IBS-D Rats by Inhibiting EGCs Activity through Regulating BDNF\/TrkB Signaling Pathway"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6634-4867","authenticated-orcid":true,"given":"Ying","family":"Zhao","sequence":"first","affiliation":[{"name":"Acupuncture and Tuina School of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610075, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5000-0725","authenticated-orcid":true,"given":"Hui-ling","family":"Jiang","sequence":"additional","affiliation":[{"name":"Acupuncture and Tuina School of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610075, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0183-4651","authenticated-orcid":true,"given":"Yu","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Chongqing Beibei Traditional Chinese Medical Hospital, Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1710-3682","authenticated-orcid":true,"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine, The People\u2019s Hospital of Shifang, Shifang, 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