{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T03:14:02Z","timestamp":1788146042909,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,8,25]],"date-time":"2022-08-25T00:00:00Z","timestamp":1661385600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2020J011085"],"award-info":[{"award-number":["2020J011085"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Computational and Mathematical Methods in Medicine"],"published-print":{"date-parts":[[2022,8,25]]},"abstract":"<jats:p>Background. lncRNAs affect adaptive and innate immunity of cancer via mediating functional states of immune cells, genes, and pathways. Nonetheless, little is known about the molecular mechanism of lncRNA-mediated CD8+ T cell immune infiltration in progression of clear cell renal cell carcinoma (ccRCC). We designed this work to investigate the role of LINC00887 in regulating CD8+ T cell immune infiltration in ccRCC. Methods. Correlation between LINC00887 and immune factors and the expression level of LINC00887 in ccRCC were analyzed by bioinformatics methods (TCGA-KIRC database, \u201cedgeR\u201d package, \u201cclusterProfiler\u201d package, and \u201cCIBERSORT\u201d package). LINC00887 expression in ccRCC was examined via RT-qPCR. The cytokilling capacity of CD8+ T cells was evaluated by the lactate dehydrogenase assay. The apoptotic ability of CD8+ T cells was measured by flow cytometry. The chemotactic ability of CD8+ T cells was revealed by chemotaxis assay. CXCR3, CXCL9, and CXCL10 levels were assessed by RT-qPCR. Results. As suggested by bioinformatics analysis, LINC00887 was markedly upregulated in ccRCC patients and associated with expression of immune-suppression molecule, thereby abating the immune infiltration level of CD8+ cells in tumor tissue. As revealed by cellular assay, LINC00887 was upregulated in ccRCC cells, and knockdown of LINC00887 resulted in a decreased PD-L1 expression, increased CD8+ T cell toxicity, decreased apoptotic levels, and enhanced chemotaxis. Moreover, we found that LINC00887 exhibited inhibitory effect on immune infiltration of CD8+ cells in clinical tissues. Conclusions. The results of this study suggested that LINC00887 promoted ccRCC progression by inhibiting immune infiltration of CD8+ T cells, providing new insights into pathogenesis of ccRCC and suggesting LINC00887 being a promising immunotherapy target for ccRCC.<\/jats:p>","DOI":"10.1155\/2022\/2582474","type":"journal-article","created":{"date-parts":[[2022,8,26]],"date-time":"2022-08-26T05:35:31Z","timestamp":1661492131000},"page":"1-17","source":"Crossref","is-referenced-by-count":8,"title":["LINC00887 Fosters Development of Clear Cell Renal Cell Carcinoma via Inhibiting CD8+ T Cell Immune Infiltration"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7814-101X","authenticated-orcid":true,"given":"Jinfeng","family":"Wu","sequence":"first","affiliation":[{"name":"The First School of Clinical Medicine, Southern Medical University, China"},{"name":"Department of Urology, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0196-8569","authenticated-orcid":true,"given":"Rongcheng","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Urology, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0596-1287","authenticated-orcid":true,"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[{"name":"The First School of Clinical Medicine, Southern Medical University, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3019-710X","authenticated-orcid":true,"given":"Yongbao","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Urology, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4431-1620","authenticated-orcid":true,"given":"Ruochen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1638-1560","authenticated-orcid":true,"given":"Wanghai","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Urology, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6754-7965","authenticated-orcid":true,"given":"Weilie","family":"Hu","sequence":"additional","affiliation":[{"name":"The First School of Clinical Medicine, Southern Medical University, China"},{"name":"Department of Urology, Guangdong Hydropower Hospital, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2015.08.007"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.639408"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1186\/s13000-020-00948-6"},{"issue":"9","key":"4","first-page":"5575","article-title":"MiR-125b-2-3p associates with prognosis of ccRCC through promoting tumor metastasis via targeting EGR1","volume":"12","author":"X. 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