{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T17:16:59Z","timestamp":1782235019435,"version":"3.54.5"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T00:00:00Z","timestamp":1667865600000},"content-version":"vor","delay-in-days":311,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005054","name":"Nantong University","doi-asserted-by":"publisher","award":["Tfj20019"],"award-info":[{"award-number":["Tfj20019"]}],"id":[{"id":"10.13039\/501100005054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>To investigate the regulatory mechanism of pterygium formation, we detected differentially expressed messenger RNAs (DE\u2010mRNAs) and differentially expressed circular RNAs (DE\u2010circRNAs) in pterygium\u2010associated conjunctival epithelium (PCE) and normal conjunctival epithelium (NCE). Genome\u2010wide mRNA and circRNA expression profiles of PCE and NCE were determined using high\u2010throughput sequencing. Bioinformatics analyses, including Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, gene set enrichment analysis (GSEA), and protein\u2013protein interaction (PPI) analysis, were conducted. The microRNAs (miRNAs) interacting with the hub DE\u2010mRNAs and DE\u2010circRNAs were predicted and verified using real\u2010time quantitative PCR (RT\u2010qPCR). The data showed that there were 536 DE\u2010mRNAs (280 upregulated and 256 downregulated mRNAs) and 78 DE\u2010circRNAs (20 upregulated and 58 downregulated circRNAs) in PCE. KEGG enrichment analysis indicated that the DE\u2010mRNAs were mainly involved in the following biological processes: IL\u201017 signalling pathway, viral protein interaction with cytokine and cytokine receptor, cytokine\u2013cytokine receptor interaction, ECM\u2010receptor interaction, and focal adhesion. The GSEA results revealed that the epithelial mesenchymal transition (EMT) process was significantly enriched in upregulated mRNAs. The pterygium\u2010associated circRNA\u2013miRNA\u2013mRNA network was established based on the top 10 DE\u2010circRNAs, 4 validated miRNAs (upregulated miR\u2010376a\u20105p and miR\u2010208a\u20105p,downregulated miR\u2010203a\u20103p and miR\u2010200b\u20103p), and 31 DE\u2010mRNAs. We found that miR\u2010200b\u20103p, as a regulator of <jats:italic>FN1<\/jats:italic>, <jats:italic>SDC2<\/jats:italic>, and <jats:italic>MEX3D<\/jats:italic>, could be regulated by 5 upregulated circRNAs. In addition, we screened out EMT\u2010related DE\u2010mRNAs, including 6 upregulated DE\u2010mRNAs and 6 downregulated DE\u2010mRNAs. The EMT\u2010related circRNA\u2013miRNA\u2013mRNA network was established with the top 10 circRNAs, 8 validated miRNAs (upregulated miR\u201017\u20105p, miR\u2010181a\u20105p, and miR\u2010106a\u20105p, downregulated miR\u2010124\u20103p, miR\u20109\u20105p, miR\u2010130b\u20105p, miR\u20101\u20103p, and miR\u201026b\u20105P), and 12 EMT\u2010related DE\u2010mRNAs. We found that hsa_circ_0002406 might upregulate <jats:italic>FN1<\/jats:italic> and <jats:italic>ADAM12<\/jats:italic> by sponging miR\u201026b\u20105p and miR\u20101\u20103p, respectively, thus promoting EMT in pterygium. Briefly, the study provides a novel viewpoint on the molecular pathological mechanisms in pterygium formation. CircRNA\u2013miRNA\u2013mRNA regulatory networks participate in the pathogenesis of pterygium and might become promising targets for pterygium prevention and treatment.<\/jats:p>","DOI":"10.1155\/2022\/2673890","type":"journal-article","created":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T19:06:29Z","timestamp":1667934389000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Identification of the circRNA\u2013miRNA\u2013mRNA Regulatory Network in Pterygium\u2010Associated Conjunctival 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