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Breast-cancer-related depression (BCRD) is associated with an increased mortality rate among breast cancer (BC) survivors. Luteolin has many pharmacological effects, particularly in the treatment of BC. In this study, we aimed to explore the anti-BCRD activity of luteolin and its underlying functional mechanism. Methods. A BCRD mouse model was induced by injecting 4T1 cells and corticosterone (COR). Behavioral test, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, Nissl staining, immunofluorescence, reverse-transcription quantitative PCR (RT-qPCR), and western blotting were used to study the effect of luteolin in mice with BCRD in vivo. A COR-induced neuron injury model was established in HT-22 cells in vitro. The role of miR-124-3p in the anti-BCRD effects of luteolin was studied using a miR-124-3p inhibitor. Results. Luteolin significantly reduced the size and weight of the tumor, increased the mice entry frequency in the symmetrical sector, and reduced the duration of immobility in the tail suspension and forced swimming tests of mice affected by BCRD. Simultaneously, apoptosis of hippocampal neurons was inhibited, and the number of Nissl bodies increased with luteolin treatment. In addition, luteolin resulted in the upregulation of miR-124-3p expression in the hippocampus and downregulated the expression of tumor necrosis factor-\u03b1 (TNF-\u03b1) and TNF receptor-associated factor 6 (TRAF6), as well as lowered the phosphorylation levels of nuclear factor-kappa B (NF-\u03baB) and IkappaB (I\u03baB). Luteolin also inhibited pyroptosis of hippocampal neurons in mice affected by BCRD, as revealed by the low protein levels of NOD-like receptor protein 3 (NLRP3), caspase-1, gasdermin D-N (GSDMD-N), interleukin (IL)-1\u03b2, and IL-18. However, the miR-124-3p inhibitor significantly reversed the therapeutic effect of luteolin on COR-induced HT-22 cells. Conclusion. Our study demonstrated that the anti-BCRD function of luteolin was mediated by regulating the miR-124-3p\/TNF-\u03b1\/TRAF6-related pathway and inhibiting neuronal cell pyroptosis and subsequent inflammation. Therefore, luteolin may be a potential drug candidate in the treatments of BCRD.<\/jats:p>","DOI":"10.1155\/2022\/2715325","type":"journal-article","created":{"date-parts":[[2022,5,6]],"date-time":"2022-05-06T17:23:42Z","timestamp":1651857822000},"page":"1-12","source":"Crossref","is-referenced-by-count":12,"title":["Luteolin Induced Hippocampal Neuronal Pyroptosis Inhibition by Regulation of miR-124-3p\/TNF-\u03b1\/TRAF6 Axis in Mice Affected by Breast-Cancer-Related Depression"],"prefix":"10.1155","volume":"2022","author":[{"given":"Qing","family":"Zhu","sequence":"first","affiliation":[{"name":"Science and Technology Innovation Center, Hunan University of Chinese Medicine, Changsha, Hunan Province 410208, China"},{"name":"Hunan Cancer Hospital\/The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan","family":"Meng","sequence":"additional","affiliation":[{"name":"Science and Technology Innovation Center, Hunan University of Chinese Medicine, Changsha, Hunan Province 410208, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanshan","family":"Han","sequence":"additional","affiliation":[{"name":"Science and Technology Innovation Center, Hunan University of Chinese Medicine, Changsha, Hunan Province 410208, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6774-2522","authenticated-orcid":true,"given":"Hui","family":"Yang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, Hunan 410007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Yang","sequence":"additional","affiliation":[{"name":"Science and Technology Innovation Center, Hunan University of Chinese Medicine, Changsha, Hunan Province 410208, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuo","family":"Liu","sequence":"additional","affiliation":[{"name":"Science and Technology Innovation Center, Hunan University of Chinese Medicine, Changsha, Hunan Province 410208, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1411-2201","authenticated-orcid":true,"given":"Yuhong","family":"Wang","sequence":"additional","affiliation":[{"name":"Science and Technology Innovation Center, Hunan University of Chinese Medicine, Changsha, Hunan Province 410208, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1025-6216","authenticated-orcid":true,"given":"Minghui","family":"Long","sequence":"additional","affiliation":[{"name":"Hunan Cancer Hospital\/The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2017.05.016"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1093\/jjco\/hyi097"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-019-05479-3"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-007-9706-5"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2019.12.005"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.2190\/pm.43.1.a"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2017.10.048"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-020-02040-8"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2019.11.005"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.12659\/msm.905580"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2018.11.029"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-260323"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-020-01254-5"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1713-z"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201700673r"},{"issue":"3","key":"16","first-page":"585","article-title":"Decreased miR-124-3p promoted breast cancer proliferation and metastasis by targeting MGAT5","volume":"9","author":"G. 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