{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:48:54Z","timestamp":1787017734836,"version":"build-2736575974"},"reference-count":34,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,10,4]],"date-time":"2022-10-04T00:00:00Z","timestamp":1664841600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Computational and Mathematical Methods in Medicine"],"published-print":{"date-parts":[[2022,10,4]]},"abstract":"<jats:p>Objective. Optic nerve glioma (ONG) is a rare disease, defined as a WHO grade I tumor, which affects the visual pathway. The objective of this study was to investigate the expression of miR-223-3p in ONG as well as its function and regulation in ONG cell lines. Methods. qRT-PCR assays were used to measure miR-223-3p expression in ONG tissues and cell lines. After overexpression of miR-223-3p in Hs683 and WERI-Rb-1 cell lines, CCK-8 and EdU assays were performed to examine cell proliferation, and flow cytometry was used to assess apoptosis. Dual luciferase assays were utilized to identify the target binding to miR-223-3p and NLRP3. Rescue assays were carried out to investigate the regulatory mechanism of miR-223-3p acting through NLRP3. Nude mouse tumorigenesis assays were established to verify the effect of miR-223-3p on ONG growth. Results. miR-223-3p was weakly expressed in both ONG tissues and cell lines. miR-223-3p inhibited the proliferative ability of Hs683 and WERI-Rb-1 cell lines and promoted apoptosis. In addition, there was binding between miR-223-3p and NLRP3. Simultaneous overexpression of NLRP3 and miR-223-3p partially counteracted the role of miR-223-3p in the cell lines. Lastly, miR-223-3p inhibited ONG growth. Conclusion. miR-223-3p plays an inhibitory role in ONG development by regulating NLRP3 to inhibit the proliferation of ONG cells and promote apoptosis.<\/jats:p>","DOI":"10.1155\/2022\/2805645","type":"journal-article","created":{"date-parts":[[2022,10,4]],"date-time":"2022-10-04T21:50:07Z","timestamp":1664920207000},"page":"1-9","source":"Crossref","is-referenced-by-count":5,"title":["miR-223-3p Regulates NLRP3 to Inhibit Proliferation and Promote Apoptosis of ONG Cells"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4710-3894","authenticated-orcid":true,"given":"Lili","family":"Nie","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, The Second Hospital of Jilin University, Changchun, 130000 Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9803-6145","authenticated-orcid":true,"given":"Hui","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The Second Hospital of Jilin University, Changchun, 130000 Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2685-2573","authenticated-orcid":true,"given":"Chi","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, The First Hospital of Jilin University, Changchun, 130021 Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3112-835X","authenticated-orcid":true,"given":"Xiaoke","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, The Second Hospital of Jilin University, Changchun, 130000 Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.2217\/cns.12.47"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.5935\/0004-2749.2021-0246"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13236032"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1055\/s-0040-1722634"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1097\/ICU.0000000000000587"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1007\/s10014-020-00383-x"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.wneu.2016.10.083"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2015.10.003"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.2174\/138920210793175895"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-015-9551-7"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1007\/s40291-019-00439-5"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.18632\/aging.203258"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.3934\/mbe.2020079"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1515\/biol-2020-0040"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0212063"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22179285"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2020.8864"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1997.86.5.0747"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.3171\/2019.2.PEDS18529"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0108950"},{"issue":"7","key":"21","first-page":"10855","article-title":"Identification of low miR-105 expression as a novel poor prognostic predictor for human glioma","volume":"8","author":"Y. 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