{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T14:54:34Z","timestamp":1780325674737,"version":"3.54.1"},"reference-count":46,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,1,29]],"date-time":"2022-01-29T00:00:00Z","timestamp":1643414400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2022,1,29]]},"abstract":"<jats:p>Background. Cryptotanshinone (CPT), an active component extracted from the root of Salvia miltiorrhiza Bunge, exhibits extensive favorable bioactive properties including anti-inflammatory, antioxidative, antibacterial, and antitumor effects. This study aims to investigate the effects of CPT on osteogenesis and explore related mechanisms both in vivo and in vitro. Methods. In the in vivo experiment, ovariectomized (OVX) female rats were intragastrically administered with CPT at doses of 10\u2009mg\/kg and 20\u2009mg\/kg for 13 consecutive weeks. Dual-energy X-ray absorptiometry was employed to detect bone mineral density (BMD). ELISA assay was leveraged to detect the biochemical parameters such as BUN and creatinine in the kidney samples. Bone and kidney sections were dyed by H&amp;E and Masson staining kits. In the in vitro experiment, the RAW 264.7 cells were stimulated through the receptor activation of the nuclear factor kappa B ligand (RANKL) to establish an osteoclast differentiation model, and CPT\u2019s protective effect against bone loss was evaluated. Differentiated osteoclasts were determined by TRAP staining. While, osteoclast-marker proteins such as NFATc1, c-Fos, and cathepsin K were identified by Western blot. Results. The results from in vivo experiments revealed that CPT could elevate bone mass and increase bone formation markers in OVX rats. Intriguingly, CPT administration noticeably ameliorated the kidney injury in OVX rats by suppressing BUN and restoring creatinine levels. Furthermore, the results from in vitro experiments suggested that CPT downregulated the protein expression of osteoclast-associated genes such as cathepsin K, c-Fos, and NFATc1 which hinted the related potential mechanisms. Conclusion. The evidence from in vivo and in vitro experiments suggested that CPT exerted antiosteoclastogenic effects by inhibiting the activation of osteoclast differentiation followed by suppressing the protein expressions of cathepsin K, c-Fos, and NFATc1 in osteoclast precursors, and it exhibited protective effects against kidney damage, which highlighted its advantage in clinical application.<\/jats:p>","DOI":"10.1155\/2022\/2821984","type":"journal-article","created":{"date-parts":[[2022,1,29]],"date-time":"2022-01-29T16:50:09Z","timestamp":1643475009000},"page":"1-8","source":"Crossref","is-referenced-by-count":8,"title":["Cryptotanshinone Suppressed Postmenopausal Osteoporosis by Preventing RANKL-Mediated Osteoclastogenesis against Kidney Injury"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8593-9727","authenticated-orcid":true,"given":"Wenjiu","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Spinal Surgery, The Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1595-2592","authenticated-orcid":true,"given":"Jing","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9603-8158","authenticated-orcid":true,"given":"Shuo","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery, First Affiliated Hospital of Shandong First Medical University (Shandong Province Qianfoshan Hospital), Jinan, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5971-0363","authenticated-orcid":true,"given":"Jun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1134-0134","authenticated-orcid":true,"given":"Tengbo","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1479-9605","authenticated-orcid":true,"given":"Jinfeng","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery, The Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","volume-title":"Osteoporosis and Metabolic Bone Disease [127\u2013142","author":"E. 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