{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:30:58Z","timestamp":1791678658766,"version":"4.3.4"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,11,12]],"date-time":"2022-11-12T00:00:00Z","timestamp":1668211200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Universidad Cient\u00edfica del Sur","award":["104-2018-FONDECYT-BM-IADT-AV"],"award-info":[{"award-number":["104-2018-FONDECYT-BM-IADT-AV"]}]},{"name":"Proyecto CONCYTEC\u2013Banco Mundial","award":["104-2018-FONDECYT-BM-IADT-AV"],"award-info":[{"award-number":["104-2018-FONDECYT-BM-IADT-AV"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Analytical Cellular Pathology"],"published-print":{"date-parts":[[2022,11,12]]},"abstract":"<jats:p>Hepatic fibrosis is caused by chronic injury due to toxic, infectious, or metabolic causes, and it may progress to cirrhosis and hepatocellular carcinoma. There is currently no antifibrotic therapy authorized for human use; however, there are promising studies using cell therapies. There are also no animal models that exactly reproduce human liver fibrosis that can be used to better understand the mechanisms of its regression and identify new targets for treatment and therapeutic approaches. On the other hand, mesenchymal stem cells (MSC) have experimentally demonstrated fibrosis regression effects, but it is necessary to have an animal model of advanced liver fibrosis to evaluate the effect of these cells. The aim of this work was to establish a protocol for the induction of advanced liver fibrosis in rats using thioacetamide (TAA), which will allow us to perform trials using MSC as a possible therapy for fibrosis regression. For this purpose, we selected 24 female rats and grouped them into three experimental groups: the control group (G-I) without treatment and groups II (G-II) and III (G-III) that received TAA by intraperitoneal injection for 24 weeks. Then, <jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mn>1<\/a:mn>\n                        <a:mo>\u00d7<\/a:mo>\n                        <a:mn>106<\/a:mn>\n                        <a:mo>\/<\/a:mo>\n                        <a:mtext>kg<\/a:mtext>\n                     <\/a:math>\n                  <\/jats:inline-formula> adipose mesenchymal stem cells (ASCs) were infused intravenously. Groups G-I and G-II were sacrificed 7 days after the last dose of ASC, and G-III was sacrificed 8 weeks after the last ASC infusion, all with xylazine\/ketamine (40\u2009mg\/kg). The protocol used in this work established a model of advanced hepatic fibrosis as corroborated by METAVIR tests of the histological lesions; by the high levels of the markers \u03b1-SMA, CD68, and collagen type I; by functional alterations due to elevated markers of the hepatic lesions; and by alterations of the leukocytes, lymphocytes, and platelets. Finally, transplanted cells in the fibrous liver were detected. We conclude that TAA applied using the protocol introduced in this study induces a good model of advanced liver fibrosis in rats.<\/jats:p>","DOI":"10.1155\/2022\/2841894","type":"journal-article","created":{"date-parts":[[2022,11,12]],"date-time":"2022-11-12T19:20:06Z","timestamp":1668280806000},"page":"1-9","source":"Crossref","is-referenced-by-count":12,"title":["Model of Liver Fibrosis Induction by Thioacetamide in Rats for Regenerative Therapy Studies"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5080-4002","authenticated-orcid":true,"given":"Nathaly","family":"Enciso","sequence":"first","affiliation":[{"name":"Grupo de Medicina Regenerativa, Universidad Cient\u00edfica del Sur, Lima 150142, Peru"},{"name":"Direcci\u00f3n General de Investigaci\u00f3n, Desarrollo e Innovaci\u00f3n, Universidad Cient\u00edfica del Sur, Lima 150142, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4455-3195","authenticated-orcid":true,"given":"Jos\u00e9","family":"Amiel","sequence":"additional","affiliation":[{"name":"Grupo de Medicina Regenerativa, Universidad Cient\u00edfica del Sur, Lima 150142, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3577-5896","authenticated-orcid":true,"given":"Fredy","family":"Fabi\u00e1n-Dom\u00ednguez","sequence":"additional","affiliation":[{"name":"Investigador Adjunto, Grupo de Medicina Regenerativa, Universidad Cient\u00edfica del Sur, Lima 150142, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5883-0814","authenticated-orcid":true,"given":"Jhon","family":"Pando","sequence":"additional","affiliation":[{"name":"Instituto de Criopreservaci\u00f3n y Terapia Celular, Lima 15074, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6451-134X","authenticated-orcid":true,"given":"Nancy","family":"Rojas","sequence":"additional","affiliation":[{"name":"Laboratorio de Microscop\u00eda Electr\u00f3nica, Universidad Nacional Mayor de San Marcos, Lima 506, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2081-6149","authenticated-orcid":true,"given":"Carlos","family":"Cisneros-Huaman\u00ed","sequence":"additional","affiliation":[{"name":"Investigador Adjunto, Grupo de Medicina Regenerativa, Universidad Cient\u00edfica del Sur, Lima 150142, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernesto","family":"Nava","sequence":"additional","affiliation":[{"name":"Laboratorio de Microscop\u00eda Electr\u00f3nica, Universidad Nacional Mayor de San Marcos, Lima 506, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9167-7329","authenticated-orcid":true,"given":"Javier","family":"Enciso","sequence":"additional","affiliation":[{"name":"Grupo de Medicina Regenerativa, Universidad Cient\u00edfica del Sur, Lima 150142, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","article-title":"Chronic liver disease","volume-title":"StatPearls, StatPearls Publishing. 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