{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T12:51:48Z","timestamp":1783947108948,"version":"3.55.0"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T00:00:00Z","timestamp":1665532800000},"content-version":"vor","delay-in-days":284,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005150","name":"Chinese Academy of Medical Sciences","doi-asserted-by":"publisher","award":["2021-PT320-001"],"award-info":[{"award-number":["2021-PT320-001"]}],"id":[{"id":"10.13039\/501100005150","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p><jats:italic>Aim<\/jats:italic>. Thin endometrium remains a severe clinical challenge with no effective therapy to date. We aimed at exploring the role and molecular mechanism of human umbilical cord mesenchymal stem cell\u2010 (hucMSC\u2010) derived exosomes (hucMSC\u2010Ex) in repairing hypoxic injury of endometrial epithelial cells (EECs). <jats:italic>Methods<\/jats:italic>. Exosomes were harvested from the conditioned medium of hucMSC and characterized using western blot, transmission electron microscopy (TEM), flow cytometry, and nanoparticle tracking analysis (NTA). EECs were subjected to hypoxic conditions before cocultured with hucMSC\u2010Ex. Cell viability, apoptosis, and migration were determined with CCK\u20108, flow cytometry, and wound healing assay, respectively. Apoptosis\/EMT\u2010related proteins were detected by western blot. The miRNA profiling was determined by RNA sequencing. The expression of miR\u2010663a and CDKN2A was measured by qRT\u2010PCR. MiR\u2010663a in EECs was overexpressed by transfecting with miR\u2010663a mimics. <jats:italic>Results<\/jats:italic>. Mesenchymal stem cells (MSCs) markers CD73, CD90, and CD106 were positively expressed in hucMSCs. Exosome isolated from hucMSC expressed CD63 and TSG101, and were 100\u2013150\u2009nm in diameter. HucMSC\u2010Ex promoted cell proliferation inhibited by hypoxia. And hucMSC\u2010Ex also inhibited hypoxia\u2010induced apoptosis, migration, and EMT of EECs by upregulating the expression of Bcl\u20102 and E\u2010cadherin and downregulating Bax and N\u2010cadherin levels. Further, bioinformatics research found that hucMSC\u2010Ex coculture can significantly upregulate the expression of miR\u2010663a and decrease the expression of CDKN2A in hypoxia\u2010induced EECs. Furthermore, miR\u2010663a overexpression inhibited CDKN2A expression and increased the expression of Bcl\u20102 and E\u2010cadherin in hypoxia\u2010induced EECs. <jats:italic>Conclusions<\/jats:italic>. HucMSC\u2010Ex promoted cell proliferation, inhibited cell apoptosis, migration, and EMT in hypoxia\u2010induced EECs, thereby alleviating hypoxia\u2010induced EECs injury, which may be related to its regulation of miR\u2010663a\/CDKN2A expression. Our study indicated that hucMSC\u2010Ex might benefit for repairing thin endometrium.<\/jats:p>","DOI":"10.1155\/2022\/3082969","type":"journal-article","created":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T20:50:32Z","timestamp":1665607832000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["[Retracted] Human Umbilical Cord Mesenchymal Stem Cell\u2010Derived Exosome Repairs Endometrial Epithelial Cells Injury Induced by Hypoxia via Regulating miR\u2010663a\/CDKN2A 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