{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T20:27:18Z","timestamp":1784838438124,"version":"3.55.0"},"reference-count":23,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T00:00:00Z","timestamp":1647388800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Healthcare Engineering"],"published-print":{"date-parts":[[2022,3,16]]},"abstract":"<jats:p>Objective. To observe the effect of carboxypeptidase E (CPE) on the ischemia and hypoxia (I\/H) injury of primary cardiomyocytes. Methods. Quantitative real-time polymerase chain reaction (qRT-PCR) technology was used to detect the expression of CPE in sham and myocardial infarction (MI) rat heart tissue, and the plasmid was transferred into primary cardiomyocytes by transfection technology. The apoptosis rate of cardiomyocytes was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining, Annexin V-PI staining, and Cell Counting Kit-8 (CCK-8) assay. In addition, Caspase kit and qRT-PCR technology were used to detect the expression of apoptosis-related factors. The cell proliferation was detected by 5-ethynyl-2\u2019-deoxyuridine (EdU) staining, flow cytometry, and qRT-PCR technology. In addition, Western blotting (WB) and qRT-PCR techniques were used to detect the Wnt\/\u03b2-catenin pathway. Results. First, we found that the expression of CPE in the marginal zone of MI was obviously reduced. Overexpression of CPE in primary cardiomyocytes can effectively inhibit ischemia\/hypoxia (I\/H)-induced apoptosis and decreased cell activity. In addition, CPE can promote cell proliferation and relieve the inhibitory effect of I\/H on cardiomyocytes. At the same time, CPE can promote the expression of \u03b2-catenin and c-myc. Conclusion. Overexpression of CPE in primary cardiomyocytes can effectively alleviate the decreased cell activity, increased apoptosis, and decreased proliferation caused by I\/H and regulated by Wnt\/\u03b2-catenin pathway.<\/jats:p>","DOI":"10.1155\/2022\/3155171","type":"journal-article","created":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T23:37:57Z","timestamp":1647473877000},"page":"1-9","source":"Crossref","is-referenced-by-count":4,"title":["CPE Regulates Proliferation and Apoptosis of Primary Myocardial Cells Mediated by Ischemia and Hypoxia Injury"],"prefix":"10.1155","volume":"2022","author":[{"given":"Jin","family":"Li","sequence":"first","affiliation":[{"name":"Department of Cardiology, The Affiliated Longyan First Hospital of Fujian Medical University, Longyan, Fujian 364000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zishuang","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Xuzhou Central Hospital, Xuzhou, Jiangsu 221000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxiong","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The Affiliated Longyan First Hospital of Fujian Medical University, Longyan, Fujian 364000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luchen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhao","sequence":"additional","affiliation":[{"name":"Division of Cardiology, The First Affiliated Hospital with Nanjing Medical University, Nanjing 210000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7992-3083","authenticated-orcid":true,"family":"Jian Zhang","sequence":"additional","affiliation":[{"name":"Division of Cardiology, The First Affiliated Hospital with Nanjing Medical University, Nanjing 210000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/s0095-4543(05)70167-6"},{"key":"2","article-title":"Acute myocardial infarction in rats","volume":"48","author":"Y. 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