{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T08:11:56Z","timestamp":1767168716117,"version":"build-2238731810"},"reference-count":14,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T00:00:00Z","timestamp":1654646400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Computational and Mathematical Methods in Medicine"],"published-print":{"date-parts":[[2022,6,8]]},"abstract":"<jats:p>\n                    Objective. To explore the effects of fibroblast growth factor 3 (FGF3) on the proliferation, cell cycle, and apoptosis of the tongue squamous cell carcinoma SCC-9 cell line (SCC-9). Methods. We measured the proliferation of SCC-9 cells in a control group, an FGF3 intervention group, and a fibroblast growth factor (FGFR) inhibitor intervention group in cholecystokinin octapeptide (CCK-8) experiments. We studied effects of FGF3 on the cell cycle and apoptosis of tongue cancer cells using flow cytometry. We further explored the IRS1\/PI3K\/AKT signaling pathway by measuring BCL-2 and Bcl-2 Associated X-protein (BAX) mRNA and protein levels with RT-PCR and western blot, respectively. Results. Results from the CCK-8 experiment showed that survival rates of cells in the control group, FGF3 intervention group, and FGFR inhibitor intervention group were\n                    <jats:inline-formula>\n                      <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mn>100.000<\/a:mn>\n                        <a:mi>%<\/a:mi>\n                        <a:mo>\u00b1<\/a:mo>\n                        <a:mn>4.026<\/a:mn>\n                        <a:mi>%<\/a:mi>\n                      <\/a:math>\n                    <\/jats:inline-formula>\n                    ,\n                    <jats:inline-formula>\n                      <c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                        <c:mn>136.330<\/c:mn>\n                        <c:mi>%<\/c:mi>\n                        <c:mo>\u00b1<\/c:mo>\n                        <c:mn>9.779<\/c:mn>\n                      <\/c:math>\n                    <\/jats:inline-formula>\n                    %, and\n                    <jats:inline-formula>\n                      <e:math xmlns:e=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\">\n                        <e:mn>83.199<\/e:mn>\n                        <e:mi>%<\/e:mi>\n                        <e:mo>\u00b1<\/e:mo>\n                        <e:mn>4.954<\/e:mn>\n                      <\/e:math>\n                    <\/jats:inline-formula>\n                    %, respectively; survival rates of SCC-9 cells in all three groups were statistically significant (\n                    <jats:inline-formula>\n                      <g:math xmlns:g=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\">\n                        <g:mi>P<\/g:mi>\n                        <g:mo>&lt;<\/g:mo>\n                        <g:mn>0.05<\/g:mn>\n                      <\/g:math>\n                    <\/jats:inline-formula>\n                    ). Compared with that in the control group, the ratio of cells in G0\/G1 phase in the FGFR inhibitor intervention group was higher (\n                    <jats:inline-formula>\n                      <i:math xmlns:i=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\">\n                        <i:mi>P<\/i:mi>\n                        <i:mo>&lt;<\/i:mo>\n                        <i:mn>0.05<\/i:mn>\n                      <\/i:math>\n                    <\/jats:inline-formula>\n                    ) and that in G2\/M phase was lower, while the FGF3 intervention group showed opposite results (\n                    <jats:inline-formula>\n                      <k:math xmlns:k=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\">\n                        <k:mi>P<\/k:mi>\n                        <k:mo>&lt;<\/k:mo>\n                        <k:mn>0.05<\/k:mn>\n                      <\/k:math>\n                    <\/jats:inline-formula>\n                    ). The apoptosis rate of tongue cancer cells differed significantly between the FGFR inhibitor intervention and the control groups (\n                    <jats:inline-formula>\n                      <m:math xmlns:m=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M7\">\n                        <m:mi>P<\/m:mi>\n                        <m:mo>&lt;<\/m:mo>\n                        <m:mn>0.05<\/m:mn>\n                      <\/m:math>\n                    <\/jats:inline-formula>\n                    ). The mRNA and protein expression levels of IRS1, PI3K, and BCL-2 were all increased in the FGF3 intervention group (\n                    <jats:inline-formula>\n                      <o:math xmlns:o=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M8\">\n                        <o:mi>P<\/o:mi>\n                        <o:mo>&lt;<\/o:mo>\n                        <o:mn>0.05<\/o:mn>\n                      <\/o:math>\n                    <\/jats:inline-formula>\n                    ), while BAX mRNA and protein expression levels were decreased (\n                    <jats:inline-formula>\n                      <q:math xmlns:q=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M9\">\n                        <q:mi>P<\/q:mi>\n                        <q:mo>&lt;<\/q:mo>\n                        <q:mn>0.05<\/q:mn>\n                      <\/q:math>\n                    <\/jats:inline-formula>\n                    ). The mRNA expression levels of protein kinase B (AKT) showed no differences between groups. The p-AKT protein was overexpressed, while the total amount of AKT protein remained stable (\n                    <jats:inline-formula>\n                      <s:math xmlns:s=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M10\">\n                        <s:mi>P<\/s:mi>\n                        <s:mo>&lt;<\/s:mo>\n                        <s:mn>0.05<\/s:mn>\n                      <\/s:math>\n                    <\/jats:inline-formula>\n                    ). Conclusion. FGF3 contributes to the proliferation of SCC-9 cells by increasing the proportion of cells in G2\/M phase. Therefore, appropriately timed inhibition of FGF3 can potentially promote tumor apoptosis through the IRS1\/PI3K\/AKT signaling pathway. Our results demonstrate the role of FGF3 in the tumor microenvironment in tongue squamous cell carcinoma SCC-9 cells and suggest new therapeutic targets.\n                  <\/jats:p>","DOI":"10.1155\/2022\/3331119","type":"journal-article","created":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T00:20:10Z","timestamp":1654734010000},"page":"1-6","source":"Crossref","is-referenced-by-count":2,"title":["Fibroblast Growth Factor 3 Is Associated with Tongue Squamous Cell Carcinoma: A Controlled Study"],"prefix":"10.1155","volume":"2022","author":[{"given":"Yang","family":"Zhang","sequence":"first","affiliation":[{"name":"Cancer Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pan","family":"Liu","sequence":"additional","affiliation":[{"name":"Cancer Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weipeng","family":"Su","sequence":"additional","affiliation":[{"name":"Cancer Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaowa","family":"Aodeng","sequence":"additional","affiliation":[{"name":"Cancer Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7247-1165","authenticated-orcid":true,"given":"Huarong","family":"Zhao","sequence":"additional","affiliation":[{"name":"Cancer Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19071875"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2016.03.024"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2021.39.15_suppl.e18010"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2013.548"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1007\/5584_2014_78"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21551"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2020.05.005"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.9734\/jpri\/2021\/v33i47B33172"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.7150\/jca.40531"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12103029"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.3390\/cells8060637"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-53907-7"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1080\/14737140.2018.1491795"},{"key":"14","article-title":"FGF\/FGFR signaling in health and disease","volume":"5","author":"Y. Xie","year":"2020","journal-title":"Signal transduction and targeted therapy"}],"updated-by":[{"DOI":"10.1155\/2023\/9784949","type":"retraction","label":"Retraction","source":"retraction-watch","updated":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T00:00:00Z","timestamp":1689724800000},"record-id":"55308"},{"DOI":"10.1155\/2023\/9784949","type":"retraction","label":"Retraction","source":"publisher","updated":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T00:00:00Z","timestamp":1689724800000}}],"container-title":["Computational and Mathematical Methods in Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/cmmm\/2022\/3331119.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/cmmm\/2022\/3331119.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/cmmm\/2022\/3331119.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T00:20:13Z","timestamp":1654734013000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/cmmm\/2022\/3331119\/"}},"subtitle":[],"editor":[{"given":"Ahmed Faeq","family":"Hussein","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2022,6,8]]},"references-count":14,"alternative-id":["3331119","3331119"],"URL":"https:\/\/doi.org\/10.1155\/2022\/3331119","relation":{},"ISSN":["1748-6718","1748-670X"],"issn-type":[{"value":"1748-6718","type":"electronic"},{"value":"1748-670X","type":"print"}],"subject":[],"published":{"date-parts":[[2022,6,8]]}}}