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Among biosafe compounds are essential oils which captured consumer draw due to their multifunctional properties compared to chemical medication drugs. Here, we examined the chemical profile and the mechanism(s) of action of the <jats:italic>Thymus vulgaris<\/jats:italic> essential oil (TVEO) against a Gram\u2010negative bacterium <jats:italic>Salmonella enterica<\/jats:italic> Typhimurium ATTCC 10028 (<jats:italic>S. enterica<\/jats:italic> Typhimurium ATTCC 10028) and two Gram\u2010positive bacteria <jats:italic>Staphyloccocus aureus<\/jats:italic> ATCC 6538 (<jats:italic>S. aureus<\/jats:italic> ATCC 6538) and <jats:italic>Listeria monocytogenes<\/jats:italic> ATCC 19117 (<jats:italic>L. monocytogenes<\/jats:italic> ATCC 19117). Findings showed that TVEO was principally composed of thymol, o\u2010cymene, and <jats:italic>\u03b3<\/jats:italic>\u2010terpinene with 47.44, 16.55, and 7.80%, respectively. Molecular docking simulations stipulated that thymol and <jats:italic>\u03b2<\/jats:italic>\u2010sesquiphellandrene (a minor compound at 1.37%) could target multiple bacterial pathways including topoisomerase II and DNA and RNA polymerases of the three tested bacteria. This result pointed plausible impairments of the pathogenic bacteria cell replication and transcription processes. Through computational approach, the VEGA quantitative structure\u2013activity relationship (QSAR) model, we revealed that among twenty\u2010six TVEO compounds, sixteen had no toxic effects and could be safe for human consumption as compared to the Food and Drug Administration (FDA) approved drugs (ciprofloxacin and rifamycin SV). Assessed by the SwissADME server, the pharmacokinetic profile of all identified TVEO compounds define their absorption, distribution, metabolism, and excretion (ADME) properties and were assessed. In order to predict their biological activity spectrum based on their chemical structure, all TVEO compounds were subjected to PASS (Prediction of Activity Spectra for Substances) online tool. Results indicated that the tested compounds could have multiple biological activities and various enzymatic targets. Findings of our study support that identified compounds of TVEO can be a safe and effective alternative to synthetic drugs and can easily combats hazardous multidrug\u2010resistant bacteria.<\/jats:p>","DOI":"10.1155\/2022\/3368883","type":"journal-article","created":{"date-parts":[[2022,7,22]],"date-time":"2022-07-22T00:20:12Z","timestamp":1658449212000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["In\u2010Depth Study of <i>Thymus vulgaris<\/i> Essential Oil: Towards Understanding the Antibacterial Target Mechanism and Toxicological and Pharmacological 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