{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T21:03:26Z","timestamp":1740171806927,"version":"3.37.3"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T00:00:00Z","timestamp":1663804800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2021JJ31068","2021YFA1101202","2021YFA1101200"],"award-info":[{"award-number":["2021JJ31068","2021YFA1101202","2021YFA1101200"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2021JJ31068","2021YFA1101202","2021YFA1101200"],"award-info":[{"award-number":["2021JJ31068","2021YFA1101202","2021YFA1101200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2022,9,22]]},"abstract":"<jats:p>The development of low-cost and effective natural products for treating neuron degenerative diseases have proven to be safe and potentially effective. Echium amoenum L. (Boraginaceae) is an annual herb that grows wildly in Europe and western Asia. The aim of this study was to evaluate the neuroprotective properties of an ethanol extract of E. amoenum L. The effects of E. amoenum L. extract on oxidative stress were measured in the rat R28 retinal precursor cell line. Furthermore, the protective role of the extract on the glutamate-induced and optic nerve crush (ONC) injury-induced cell death were evaluated in vitro and in vivo, respectively. Our results showed that the ethanol extract of E. amoenum L. prevented the glutamate-induced decrease in cell viability and increase in cell death in R28 cells and suppressed the overproduction of ROS induced by glutamate. Moreover, the extract significantly inhibited microglial activation and optic nerve damage induced by ONC injury in mice. In addition, the mechanism was attributed to the ability of the extract to decrease NF-\u03baB pathway activation and its downstream inflammatory cytokine production. In conclusion, E. amoenum L. ethanol extract had a potent neuroprotective effect against glutamate-induced and ONC-induced cell death. This is likely due to its antioxidant and anti-inflammatory properties.<\/jats:p>","DOI":"10.1155\/2022\/3631532","type":"journal-article","created":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T17:52:38Z","timestamp":1663869158000},"page":"1-11","source":"Crossref","is-referenced-by-count":3,"title":["Echium amoenum L. Ethanol Extract Protects Retinal Ganglion Cell after Glutamate and Optic Nerve Crush Injury"],"prefix":"10.1155","volume":"2022","author":[{"given":"Haibo","family":"Li","sequence":"first","affiliation":[{"name":"Eye Center of Xiangya Hospital and Hunan Key Laboratory of Ophthalmology, Central South University, Changsha, 410008 Hunan Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ghazaleh","family":"Behnammanesh","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenkai","family":"Wu","sequence":"additional","affiliation":[{"name":"The First People\u2019s Hospital of Changde, Changde City, 415000 Hunan Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Rong","sequence":"additional","affiliation":[{"name":"Eye Center of Xiangya Hospital and Hunan Key Laboratory of Ophthalmology, Central South University, Changsha, 410008 Hunan Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengling","family":"You","sequence":"additional","affiliation":[{"name":"Eye Center of Xiangya Hospital and Hunan Key Laboratory of Ophthalmology, Central South University, Changsha, 410008 Hunan Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aman Shah Abdul","family":"Majid","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Quest International University Perak, Ipoh, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5901-3672","authenticated-orcid":true,"given":"Dan","family":"Ji","sequence":"additional","affiliation":[{"name":"Eye Center of Xiangya Hospital and Hunan Key Laboratory of Ophthalmology, Central South University, Changsha, 410008 Hunan Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-016-0495-1"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100617-062508"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10040608"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-30018-9_94"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/8152373"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2020.113518"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.4103\/phrev.phrev_13_18"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1080\/10942912.2016.1233436"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awu037"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10121948"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2019.00219"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.18-24773"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.247463"},{"key":"14","first-page":"101","article-title":"A Lack of tolerance to the anxiolytic action of Echium amoenum","volume":"6","author":"M. Rabbani","year":"2011","journal-title":"Research in Pharmaceutical Sciences"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1080\/01480545.2017.1317785"},{"volume-title":"Gas Chromatography and Mass Spectrometry: A Practical Guide: A Practical Guide","year":"2011","author":"O. D. Sparkman","key":"16"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1021\/jf500180u"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1021\/jf302218g"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.3791\/2685"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.06.031"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.28735"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-010-0249-5"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.4103\/1735-5362.220970"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.2174\/1570163815666180219115844"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.3233\/JIN-180093"},{"issue":"2","key":"26","first-page":"157","article-title":"Cytoprotective and antioxidant effects of Echium amoenum anthocyanin-rich extract in human endothelial cells (HUVECs)","volume":"5","author":"L. Safaeian","year":"2015","journal-title":"Avicenna Journal of Phytomedicine"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines6020048"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1007\/s10059-011-0042-6"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2021.108805"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2013.10.034"},{"key":"31","first-page":"2191","article-title":"Formononetin attenuates hydrogen peroxide (H2O2)-induced apoptosis and NF-kappaB activation in RGC-5 cells","volume":"18","author":"W. C. Jia","year":"2014","journal-title":"European Review for Medical and Pharmacological Sciences"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.10.090"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1007\/s00005-016-0405-y"}],"container-title":["Disease Markers"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/dm\/2022\/3631532.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/dm\/2022\/3631532.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/dm\/2022\/3631532.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T17:52:53Z","timestamp":1663869173000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/dm\/2022\/3631532\/"}},"subtitle":[],"editor":[{"given":"Yi","family":"Shao","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2022,9,22]]},"references-count":33,"alternative-id":["3631532","3631532"],"URL":"https:\/\/doi.org\/10.1155\/2022\/3631532","relation":{},"ISSN":["1875-8630","0278-0240"],"issn-type":[{"type":"electronic","value":"1875-8630"},{"type":"print","value":"0278-0240"}],"subject":[],"published":{"date-parts":[[2022,9,22]]}}}