{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:05:53Z","timestamp":1790859953733,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T00:00:00Z","timestamp":1662508800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81860651"],"award-info":[{"award-number":["81860651"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["20200201492JC"],"award-info":[{"award-number":["20200201492JC"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011789","name":"Department of Science and Technology of Jilin Province","doi-asserted-by":"publisher","award":["81860651"],"award-info":[{"award-number":["81860651"]}],"id":[{"id":"10.13039\/501100011789","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011789","name":"Department of Science and Technology of Jilin Province","doi-asserted-by":"publisher","award":["20200201492JC"],"award-info":[{"award-number":["20200201492JC"]}],"id":[{"id":"10.13039\/501100011789","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2022,9,7]]},"abstract":"<jats:p>Purpose. Rheumatoid arthritis (RA) shows abnormal proliferation, apoptosis, and invasion in fibroblast-like synoviocytes (FLSs). Baicalein (BAI), extracted from Scutellaria baicalensis, is used as an anticancer drug through inducing cancer cells apoptosis. However, the mechanism of BAI in RA progression still remains unknown. Here, we demonstrated that BAI inhibited FLS proliferation and migration, whereas it enhanced apoptosis via the PI3K\/Akt\/mTOR pathway in vitro. Methods. Cell viability and colony formation were analyzed by MTT and plate colony formation assays in SW982\u2009cells, respectively. Apoptosis was detected by flow cytometry and western blotting. Epithelial-mesenchymal transition (EMT), MMP family proteins (MMP2\/9), and the PI3K\/Akt\/mTOR pathway were detected by western blot. Cell migration was detected by scratch healing assay under BAI treatment in SW982\u2009cells. Results. BAI dose-dependently inhibited cell viability and colony forming in SW982\u2009cells. BAI upregulated apoptotic proteins and downregulated EMT-related proteins, resulting in enhanced cell apoptosis and inhibited cell migration in SW982 cells. BAI also dose-dependently inhibited the phosphorylation of PI3K, Akt, and mTOR. Conclusions. These results indicated that BAI inhibited FLSs proliferation and EMT, whereas induced cell apoptosis through blocking the PI3K\/Akt\/mTOR pathway, supporting clinical application for RA progression.<\/jats:p>","DOI":"10.1155\/2022\/3643265","type":"journal-article","created":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T21:35:10Z","timestamp":1662586510000},"page":"1-9","source":"Crossref","is-referenced-by-count":11,"title":["Baicalein Induces Apoptosis of Rheumatoid Arthritis Synovial Fibroblasts through Inactivation of the PI3K\/Akt\/mTOR Pathway"],"prefix":"10.1155","volume":"2022","author":[{"given":"Xue","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Rheumatology, Affiliated Hospital of Yanbian University, Yanji 133002, Jilin Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Affiliated Hospital of Yanbian University, Yanji 133002, Jilin Province, China"},{"name":"Department of Kidney Disease & Rheumatology, Tongliao City Hospital, Tongliao 028000, Inner Mongolia Autonomous Region, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingshi","family":"Piao","sequence":"additional","affiliation":[{"name":"Laboratory of Cancer Research Center, Yanbian University Medical College, Yanji 133002, Jilin Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangguo","family":"Che","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, Korea Mouse Phenotyping Center, School of Medicine, Kyungpook National University, Daegu, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengge","family":"Si","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Affiliated Hospital of Yanbian University, Yanji 133002, Jilin Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3985-2513","authenticated-orcid":true,"given":"Jingchun","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Affiliated Hospital of Yanbian University, Yanji 133002, Jilin Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"issue":"1","key":"1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/s13075-018-1794-6","article-title":"Cytosolic group IVA phospholipase A2 inhibitors, AVX001 and AVX002, ameliorate collagen-induced arthritis","volume":"21","author":"A. J. Feuerherm","year":"2019","journal-title":"Arthritis Research and Therapy"},{"issue":"6","key":"2","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1186\/ar2337","article-title":"Cells of the synovium in rheumatoid arthritis. Synovial fibroblasts","volume":"9","author":"U. Muller-Ladner","year":"2007","journal-title":"Arthritis Research and Therapy"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1186\/ar4357"},{"issue":"2","key":"4","first-page":"317","article-title":"Fibroblasts as sentinel cells. Synthesis of chemokines and regulation of inflammation","volume":"151","author":"R. S. Smith","year":"1997","journal-title":"American Journal Of Pathology"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.113126"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.3390\/biom9120795"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.114368"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.3390\/toxins11090505"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.2174\/0929867322666141212122352"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2017.03.004"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1142\/s0192415x21500245"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/5527190"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2021.115594"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.788392"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-001-2138-6"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2019.10617"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.2147\/jir.s302929"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1002\/art.40398"},{"issue":"4","key":"19","first-page":"554","article-title":"Baseline serum immunoglobulin levels in patients with rheumatoid arthritis: relationships with clinical parameters and with B-cell dynamics following rituximab","volume":"30","author":"I. de la Torre","year":"2012","journal-title":"Clinical & Experimental Rheumatology"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-017-0631-3"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2019.05.024"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-017-2035-2"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2020.114302"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2008.09.005"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2020.11369"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/5786053"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.2147\/ott.s282790"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1186\/ar4112"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2008.11.003"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1038\/nri802"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kel065"},{"issue":"1","key":"32","first-page":"117","article-title":"Validity of SW982 synovial cell line for studying the drugs against rheumatoid arthritis in fluvastatin-induced apoptosis signaling model","volume":"139","author":"J. H. Chang","year":"2014","journal-title":"Indian Journal of Medical Research"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2012.01.030"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2015.172"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1186\/ar69"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0802826"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1042\/bsr20180890"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-012-1463-z"},{"issue":"1","key":"39","first-page":"73","article-title":"Effect of moxibustion on autophagy and the inflammatory response of synovial cells in rheumatoid arthritis model rat","volume":"42","author":"F. Hao","year":"2022","journal-title":"Journal of Traditional Chinese Medicine"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1080\/21655979.2022.2062969"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/1988378"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.12017"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1101\/gad.225334.113"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-9-325"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.3892\/or_00000638"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1016\/0272-6386(95)90610-x"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.2147\/dddt.s102541"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.714566"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-019-1935-6"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1186\/s13018-021-02887-4"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2015.3935"}],"container-title":["Evidence-Based Complementary and Alternative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2022\/3643265.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2022\/3643265.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2022\/3643265.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T21:35:35Z","timestamp":1662586535000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/ecam\/2022\/3643265\/"}},"subtitle":[],"editor":[{"given":"Talha Bin","family":"Emran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,9,7]]},"references-count":51,"alternative-id":["3643265","3643265"],"URL":"https:\/\/doi.org\/10.1155\/2022\/3643265","relation":{},"ISSN":["1741-4288","1741-427X"],"issn-type":[{"value":"1741-4288","type":"electronic"},{"value":"1741-427X","type":"print"}],"subject":[],"published":{"date-parts":[[2022,9,7]]}}}