{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T17:04:52Z","timestamp":1790960692935,"version":"4.1.0"},"reference-count":82,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,10]],"date-time":"2022-09-10T00:00:00Z","timestamp":1662768000000},"content-version":"vor","delay-in-days":252,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Silver nanoparticles (AgNPs) utilization is becoming increasingly popular. The existing investigation evaluates the ameliorative impact of eugenol (Eug) against the toxic influences of AgNPs on rats\u2019 liver. <jats:italic>Methods<\/jats:italic>. Sixty adult male rats were enrolled equally into control, Eug (100\u2009mg\u2009kg<jats:sup>-1<\/jats:sup> orally), AgNPs\u2010low dose (1\u2009mg\u2009kg<jats:sup>-1<\/jats:sup> i.p), AgNPs\u2010high dose (2\u2009mg\u2009kg<jats:sup>-1<\/jats:sup> i.p), Eug\u2009+\u2009AgNPs\u2010low dose (100\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup>\u2009orally + 1\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup>\u2009i.p), and Eug\u2009+\u2009AgNPs high dose (100\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup>\u2009orally + 2\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup>\u2009i.p). All the groups were treated daily for 30 days, subsequently serum aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total protein, total albumin, lactate dehydrogenase (LDH), total oxidative capacity (TOC), malondialdehyde (MDA), tumor necrosis factor\u2010alpha (TNF\u2010<jats:italic>\u03b1<\/jats:italic>), total antioxidant capacity (TAC), and interleukin 6 (IL\u20106) levels were measured; hepatic tissues superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and glutathione peroxidase (GPx) levels were evaluated; histopathology and histomorphometry were documented in the liver of all groups; and Bcl\u20102, P53, Caspase\u20103, and TNF\u2010<jats:italic>\u03b1<\/jats:italic> reactive proteins were also immunohistochemically detected. <jats:italic>Results<\/jats:italic>. AgNPs significantly triggered oxidative stress in hepatic tissues, characterized by elevated levels of AST, ALT, ALP, LDH, TOC, MDA, TNF\u2010<jats:italic>\u03b1<\/jats:italic>, and IL\u20106 correlating with considerable decline in total protein, total albumin, TAC, SOD, CAT, GSH, and GPx. These changes were paralleled with histopathological alterations remarkable by devastation of the ordinary hepatic structure, with decrease in the numbers of normal hepatocytes, elevation in the numbers of necrotic hepatocytes, periportal and centrilobular inflammatory cells, deteriorated Kupffer cells, and dilated\/congested central and portal veins. Alongside, a marked diminution in Bcl\u20102 immunoreactivity and a significant elevation in P53, Caspase\u20103, and TNF\u2010<jats:italic>\u03b1<\/jats:italic> immunoreactivities were recorded. Supplementation of AgNPs\u2010treated animals with Eug reversed most of the biochemical, histopathological, and immunohistochemical changes. <jats:italic>Conclusion<\/jats:italic>. This study proposed that Eug has an ameliorative effect against AgNPs\u2010induced hepatotoxicity.<\/jats:p>","DOI":"10.1155\/2022\/3820848","type":"journal-article","created":{"date-parts":[[2022,9,10]],"date-time":"2022-09-10T19:50:21Z","timestamp":1662839421000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["The Ameliorative Role of Eugenol against Silver Nanoparticles\u2010Induced Hepatotoxicity in Male Wistar Rats"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9795-2363","authenticated-orcid":false,"given":"Hany N.","family":"Yousef","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somaya S.","family":"Ibraheim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramadan A.","family":"Ramadan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3073-000X","authenticated-orcid":false,"given":"Hanaa R.","family":"Aboelwafa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,10]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1166\/jbn.2011.1324"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.etp.2014.09.005"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.2174\/13852728113176660141"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2018.04.006"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fpsl.2018.03.008"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2017.12.003"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/es103302t"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0578350"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/wnan.1325"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21072375"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfs306"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-014-2252-7"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfda.2014.01.010"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/8748253"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1039\/C7RA04803C"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/1476-0711-8-9"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf0303114"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2007.07.050"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1089\/cbr.2017.2181"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1088\/2053-1591\/ab30a4"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17091534"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-9244-4"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0300-483X(95)03199-P"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_9_25_2","volume-title":"Fundamentals of Clinical Chemistry","author":"Tietz N. W.","year":"1976"},{"key":"e_1_2_9_26_2","first-page":"686","article-title":"Protocole de validation de techniques. Commission Validation de techniques","volume":"44","author":"Vassault A.","year":"1986","journal-title":"Annales de Biologie Clinique"},{"key":"e_1_2_9_27_2","volume-title":"Physiological Chemistry Laboratory Directions","author":"Armstrong W.","year":"1964"},{"key":"e_1_2_9_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(71)90365-2"},{"key":"e_1_2_9_29_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.54.5.356"},{"key":"e_1_2_9_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2697(02)00652-8"},{"key":"e_1_2_9_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(78)52032-6"},{"key":"e_1_2_9_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(72)80218-3"},{"key":"e_1_2_9_33_2","first-page":"121","volume-title":"Methods in Enzymology","author":"Aebi H.","year":"1984"},{"key":"e_1_2_9_34_2","first-page":"882","article-title":"Improved method for the determination of blood glutathione","volume":"61","author":"Beutler E.","year":"1963","journal-title":"The Journal of Laboratory and Clinical Medicine"},{"key":"e_1_2_9_35_2","first-page":"158","article-title":"Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase","volume":"70","author":"Paglia D. E.","year":"1967","journal-title":"The Journal of Laboratory and Clinical Medicine"},{"key":"e_1_2_9_36_2","unstructured":"BancroftJ. D.andGambleM. Theory and Practice of Histological Techniques 2008 Sixth edition Edinburgh: Churchill Livingstone."},{"key":"e_1_2_9_37_2","doi-asserted-by":"publisher","DOI":"10.1179\/014788802794791951"},{"key":"e_1_2_9_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep42964"},{"key":"e_1_2_9_39_2","doi-asserted-by":"publisher","DOI":"10.1080\/24701556.2020.1729191"},{"key":"e_1_2_9_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2022.04.103"},{"key":"e_1_2_9_41_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-019-3877-8"},{"key":"e_1_2_9_42_2","doi-asserted-by":"publisher","DOI":"10.1186\/s43088-019-0031-y"},{"key":"e_1_2_9_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mseb.2020.114770"},{"key":"e_1_2_9_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jrras.2015.10.002"},{"key":"e_1_2_9_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jenvman.2020.111918"},{"key":"e_1_2_9_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2005.06.034"},{"key":"e_1_2_9_47_2","doi-asserted-by":"publisher","DOI":"10.17795\/ajmb-35557"},{"key":"e_1_2_9_48_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13204-013-0229-6"},{"key":"e_1_2_9_49_2","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.1236"},{"key":"e_1_2_9_50_2","doi-asserted-by":"publisher","DOI":"10.1002\/jat.1057"},{"key":"e_1_2_9_51_2","doi-asserted-by":"publisher","DOI":"10.24125\/sanamed.v14i2.336"},{"key":"e_1_2_9_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.111588"},{"key":"e_1_2_9_53_2","doi-asserted-by":"publisher","DOI":"10.1080\/1040841X.2017.1295225"},{"key":"e_1_2_9_54_2","doi-asserted-by":"publisher","DOI":"10.4414\/smw.2012.13659"},{"key":"e_1_2_9_55_2","volume-title":"Endogenous Antioxidants: A Review of their Role in Oxidative Stress","author":"Aguilar T. A. F.","year":"2016"},{"key":"e_1_2_9_56_2","volume-title":"Biochemistry of Reactive Oxygen and Nitrogen Species, in Basic Principles and Clinical Significance of Oxidative Stress","author":"Ozcan A.","year":"2015"},{"key":"e_1_2_9_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2016.06.016"},{"key":"e_1_2_9_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(94)92211-X"},{"key":"e_1_2_9_59_2","first-page":"6207","article-title":"Histopathologic and apoptotic effect of nanosilver in liver of broiler chickens","volume":"11","author":"Loghman A.","year":"2012","journal-title":"African Journal of Biotechnology"},{"key":"e_1_2_9_60_2","doi-asserted-by":"publisher","DOI":"10.21608\/blj.2019.47569"},{"key":"e_1_2_9_61_2","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.48.1467"},{"key":"e_1_2_9_62_2","volume-title":"Involvement of Cytokines in the Pathogenesis of Systemic Lupus Erythematosus, in Cytokines and Chemokines in Autoimmune Disease","author":"Lauwerys B. R.","year":"2003"},{"key":"e_1_2_9_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2013.07.071"},{"key":"e_1_2_9_64_2","doi-asserted-by":"publisher","DOI":"10.1080\/00207454.2016.1212854"},{"key":"e_1_2_9_65_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3893"},{"key":"e_1_2_9_66_2","doi-asserted-by":"publisher","DOI":"10.2174\/1874070702014010113"},{"key":"e_1_2_9_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mehy.2004.08.028"},{"key":"e_1_2_9_68_2","doi-asserted-by":"publisher","DOI":"10.4172\/2161-0495.s4-001"},{"key":"e_1_2_9_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(92)90411-U"},{"key":"e_1_2_9_70_2","first-page":"358","article-title":"Modulation of Kupffer cell activity: physio-pathological consequences on hepatic metabolism","volume":"159","author":"Neyrinck A.","year":"2004","journal-title":"Bulletin et M\u00e9moires de l'Acad\u00e9mie Royale de M\u00e9decine de Belgique"},{"key":"e_1_2_9_71_2","first-page":"31","article-title":"Inflammatory response, reactive oxygen species, programmed (necrotic-like and apoptotic) cell death and cancer","volume":"49","author":"Johar D.","year":"2004","journal-title":"Roczniki Akademii Medycznej w Bia\u0142ymstoku"},{"key":"e_1_2_9_72_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.2004.03975.x"},{"key":"e_1_2_9_73_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc883"},{"key":"e_1_2_9_74_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203285"},{"key":"e_1_2_9_75_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-018-1287-0"},{"key":"e_1_2_9_76_2","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.12059"},{"key":"e_1_2_9_77_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacme.2015.09.005"},{"key":"e_1_2_9_78_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2010.12.010"},{"key":"e_1_2_9_79_2","first-page":"219","article-title":"Studies on free radicals, antioxidants, and co-factors","volume":"2","author":"Rahman K.","year":"2007","journal-title":"Clinical Interventions in Aging"},{"key":"e_1_2_9_80_2","doi-asserted-by":"publisher","DOI":"10.22037\/IJPR.2020.1101026"},{"key":"e_1_2_9_81_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40139-015-0093-z"},{"key":"e_1_2_9_82_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0528.2002.017420.x"}],"container-title":["Oxidative Medicine and Cellular Longevity"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2022\/3820848.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2022\/3820848.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2022\/3820848","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T23:51:28Z","timestamp":1748908288000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2022\/3820848"}},"subtitle":[],"editor":[{"given":"Cassiano Felippe","family":"Gon\u00e7alves-de-Albuquerque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,1]]},"references-count":82,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1155\/2022\/3820848"],"URL":"https:\/\/doi.org\/10.1155\/2022\/3820848","archive":["Portico"],"relation":{},"ISSN":["1942-0900","1942-0994"],"issn-type":[{"value":"1942-0900","type":"print"},{"value":"1942-0994","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1]]},"assertion":[{"value":"2022-04-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"3820848"}}