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The traditional Chinese medicine (TCM) compound prescription, Dian Dao San (DDS), is effective for treating P. acnes. Previous clinical work by our team demonstrated that improved Dian Dao San (IDDS) has better antibacterial effects. However, the mechanism of IDDS inhibition of P. acnes is still unknown. Hence, the isobaric tags for relative and absolute quantitation (iTRAQ) technology was applied to explore the antibacterial mechanism of IDDS against P. acnes. Our results suggested that the antibacterial mechanism of IDDS was related to the glycolytic pathway. gap, pgk, and tpiA enzymes were found to be potential target proteins in the bacterial glycolytic pathway as an antibacterial mechanism of inhibition. In addition, SEM and TEM analyses revealed that IDDS may destruct bacterial plasma membrane and cell wall. The results provide a reliable, direct, and scientific theoretical basis for wide application of IDDS.<\/jats:p>","DOI":"10.1155\/2022\/3855702","type":"journal-article","created":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T22:20:10Z","timestamp":1644445210000},"page":"1-9","source":"Crossref","is-referenced-by-count":5,"title":["Proteomic Analysis of the Antibacterial Effect of Improved Dian Dao San against Propionibacterium acnes"],"prefix":"10.1155","volume":"2022","author":[{"given":"Shuaijun","family":"Ren","sequence":"first","affiliation":[{"name":"Guizhou University of Traditional Chinese Medicine, No. 50, Shidong Rd., Nanming District, Guiyang City, Guizhou Province 550001, China"},{"name":"Dermatology Department, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, No. 71, Baoshan North Rd., Yunyan District, Guiyang City, Guizhou Province 550002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjiao","family":"Wang","sequence":"additional","affiliation":[{"name":"Ethnic Medicine, Qiannan Traditional Chinese Medicine Hospital, No. 32 Jianjiang Middle Rd., Duyun City, Guizhou Province 558000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4980-4715","authenticated-orcid":true,"given":"Min","family":"Jia","sequence":"additional","affiliation":[{"name":"Dermatology Department, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, No. 71, Baoshan North Rd., Yunyan District, Guiyang City, Guizhou Province 550002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lili","family":"An","sequence":"additional","affiliation":[{"name":"Guizhou University of Traditional Chinese Medicine, No. 50, Shidong Rd., Nanming District, Guiyang City, Guizhou Province 550001, China"},{"name":"Dermatology Department, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, No. 71, Baoshan North Rd., Yunyan District, Guiyang City, Guizhou Province 550002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6209-1794","authenticated-orcid":true,"given":"Ting","family":"Tang","sequence":"additional","affiliation":[{"name":"Guizhou University of Traditional Chinese Medicine, No. 50, Shidong Rd., Nanming District, Guiyang City, Guizhou Province 550001, China"},{"name":"Dermatology Department, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, No. 71, Baoshan North Rd., Yunyan District, Guiyang City, Guizhou Province 550002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5578-3450","authenticated-orcid":true,"given":"Xin","family":"Liu","sequence":"additional","affiliation":[{"name":"Guizhou University of Traditional Chinese Medicine, No. 50, Shidong Rd., Nanming District, Guiyang City, Guizhou Province 550001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1080\/19381980.2017.1356519"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijwd.2017.10.006"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.7417\/CT.2014.1731"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/s0254-6272(17)30326-6"},{"issue":"002","key":"5","first-page":"180","article-title":"Research progress in the treatment of acne vulgaris","volume":"018","author":"T. 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