{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T22:24:32Z","timestamp":1770330272602,"version":"3.49.0"},"reference-count":23,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,2,26]],"date-time":"2022-02-26T00:00:00Z","timestamp":1645833600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Healthcare Engineering"],"published-print":{"date-parts":[[2022,2,26]]},"abstract":"Objective. We aimed at exploring the role of let-7a in the pathogenesis of pediatric Henoch\u2013Sch\u00f6nlein purpura (HSP) and its related mechanism. Methods. Fifty-five pediatric HSP patients and 20 paired healthy controls were included. The expressions of let-7a and TNFAIP3 were detected by RT-qPCR or\/and western blot. Vessel fibrinoid necrosis was evaluated in skin tissues by PTAH staining. The serum IgA level was measured by ELISA. Cells were transfected with let-7a inhibitor and\/or TNFAIP3 siRNA, accompanied by pretreatment with NF-\u03baB inhibitor PDTC or not. After being cultured in HSP serum, the changes in cell viability, cell apoptosis, apoptosis-related proteins, and NF-\u03baB pathway-related proteins were detected by CCK8, flow cytometry, and western blot. Results. The let-7a expression level was positively correlated with the serum IgA level and severity degree of vascular fibrinoid necrosis in HSP patients. Let-7a expression was significantly increased, whereas cell viability and TNFAIP3 expression were obviously decreased 48\u2009h after HUVECs were incubated with HSP serum. Let-7a knockdown upregulated the cell viability, whereas it reduced the apoptotic ratio, apoptosis protein expressions (Bax\/Bcl2 ratio, cleaved-caspase 3), and NF-\u03baB pathway activation (reflected by reduced P65 nuclear translocation and p-I\u03baB\u03b1 expression) in HUVECs (all \n \n p<\/a:mi>\n <<\/a:mo>\n 0.05<\/a:mn>\n <\/a:math>\n <\/jats:inline-formula>). The changes induced by let-7a knockdown were obviously reversed by TNFAIP3 siRNA transfection \n \n \n \n p<\/c:mi>\n <<\/c:mo>\n 0.05<\/c:mn>\n <\/c:mrow>\n <\/c:mfenced>\n <\/c:math>\n <\/jats:inline-formula>. Besides, PDTC treatment remarkably diminished the anti-apoptosis effect of let-7a knockdown and pro-apoptosis effect of TNFAIP3 siRNA on HUVECs induced by HSP serum. Conclusions. Let-7a knockdown significantly suppressed vascular endothelial cell apoptosis induced by HSP serum by targeting TNFAPI3 via NF-\u03baB signaling pathway. Our findings provided a potential therapeutic target for the treatment of HSP.<\/jats:p>","DOI":"10.1155\/2022\/3889318","type":"journal-article","created":{"date-parts":[[2022,2,26]],"date-time":"2022-02-26T18:05:08Z","timestamp":1645898708000},"page":"1-10","source":"Crossref","is-referenced-by-count":3,"title":["Let-7a Targeting TNFAPI3 Promotes Vascular Endothelial Cell Apoptosis of Pediatric Patients with Henoch\u2013Sch\u00f6nlein Purpura via NF-\u03baB Signaling Pathway"],"prefix":"10.1155","volume":"2022","author":[{"given":"Mingming","family":"Cui","sequence":"first","affiliation":[{"name":"First Clinical College, Shandong University of Traditional Chinese Medicine, No. 4655, Daxue Road, University Science Park, Changqing District, Jinan, Shandong 250355, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jilong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Cao County People\u2019s Hospital, Qinghe Road East, Fumin Avenue South, Caoxian Development District, Heze, Shandong 274400, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Geng","sequence":"additional","affiliation":[{"name":"Department of Physiotherapy, Jinan City People\u2019s Hospital, No. 001, Xuehu Street, Laiwu District, Jinan, Shandong 271199, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianqian","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Second Internal Medicine, Jinan Shizhong People\u2019s Hospital, No. 61, Langmaoshan Road, Jinan, Shandong 250002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8970-0526","authenticated-orcid":true,"given":"Leiming","family":"Xi","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, No. 42, Wenhuaxi Road, Jinan, Shandong 250011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2019.00257"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1097\/00002281-200101000-00006"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780400513"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2634"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2011.01.007"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.3390\/cells9020276"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.can-07-0563"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2006.01.025"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2019.00808"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.31557\/apjcp.2020.21.2.555"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1200822"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2019.10493"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5488.2350"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1007\/s12519-019-00318-3"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtemb.2019.03.008"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3459"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0107655"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2017.11.006"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1111\/his.13927"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.86.9.974"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1186\/1423-0127-20-3"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1097\/00002281-200009000-00001"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0121256"}],"container-title":["Journal of Healthcare Engineering"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jhe\/2022\/3889318.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jhe\/2022\/3889318.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jhe\/2022\/3889318.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,26]],"date-time":"2022-02-26T18:05:10Z","timestamp":1645898710000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jhe\/2022\/3889318\/"}},"subtitle":[],"editor":[{"given":"Kalidoss","family":"Rajakani","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2022,2,26]]},"references-count":23,"alternative-id":["3889318","3889318"],"URL":"https:\/\/doi.org\/10.1155\/2022\/3889318","relation":{},"ISSN":["2040-2309","2040-2295"],"issn-type":[{"value":"2040-2309","type":"electronic"},{"value":"2040-2295","type":"print"}],"subject":[],"published":{"date-parts":[[2022,2,26]]}}}