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Preexisting conditions which increase oxidative stress, e.g., hypertension and diabetes, increase the mother\u2019s risk to develop preeclampsia. Previously, we established that when the extracellular matrix is exposed to oxidative stress, trophoblast function is impaired, and this may lead to improper placentation. We investigated how the oxidative ECM present in preeclampsia alters the behavior of first trimester extravillous trophoblasts. We demonstrate elevated levels of advanced glycation end products (AGE) and lipid oxidation end product 4-hydroxynonenal in preeclamptic ECM (28%, and 32% increase vs control, respectively) accompanied with 35% and 82% more 3-chlorotyrosine and 3-nitrotyrosine vs control, respectively. Furthermore, we hypothesized that 670\u2009nm phototherapy, which has antioxidant properties, reverses the observed trophoblast dysfunction as depicted in the improved migration and reduction in apoptosis. Since NO is critical for placentation, we examined eNOS activity in preeclamptic placentas compared to healthy ones and found no differences; however, 670\u2009nm light treatment triggered enhanced NO availability presumably by using alternative NO sources. Light exposure decreased apoptosis and restored trophoblast migration to levels in trophoblasts cultured on preeclamptic ECM. Moreover, 670\u2009nm irradiation restored expression of Transforming Growth Factor (TGF\u03b2) and Placental Growth Factor (PLGF) to levels observed in trophoblasts cultured on healthy placental ECM. We conclude the application of 670\u2009nm light can successfully mitigate the damaged placental microenvironment of late onset preeclampsia as depicted by the restored trophoblast behavior.<\/jats:p>","DOI":"10.1155\/2022\/3922368","type":"journal-article","created":{"date-parts":[[2022,4,20]],"date-time":"2022-04-20T19:05:13Z","timestamp":1650481513000},"page":"1-10","source":"Crossref","is-referenced-by-count":2,"title":["Red Light Mitigates the Deteriorating Placental Extracellular Matrix in Late Onset of Preeclampsia and Improves the Trophoblast Behavior"],"prefix":"10.1155","volume":"2022","author":[{"given":"Jakara","family":"Griffin","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John G.","family":"Krolikowski","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Medical College of 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