{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T10:35:43Z","timestamp":1769164543108,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,6,18]],"date-time":"2022-06-18T00:00:00Z","timestamp":1655510400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Program of Innovative Subject of Young People in Medical Research in Sichuan Province","award":["Q17009"],"award-info":[{"award-number":["Q17009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Computational and Mathematical Methods in Medicine"],"published-print":{"date-parts":[[2022,6,18]]},"abstract":"<jats:p>Growing evidence has demonstrated that high heterogeneity contributes to poor prognosis and malignancies. The existence of melanoma cancer stem-like cells (CSCs), which are a small subpopulation of melanoma cells, is responsible for tumour resistance to therapies. Recently, plant secondary metabolites have attracted attention because they are considered promising compounds that are isolated from herbs that could help to target different subpopulations of tumours. In the present study, we aimed to identify the antitumourigenic activities of the medicinal compound chelerythrine chloride (CHE) on melanoma CSCs. CHE (30-40\u2009\u03bcmol\/L) induced apoptosis in A375 and A2058 CSCs. A relatively low dose of CHE (1-5\u2009\u03bcmol\/L) inhibited the stemness of melanoma CSCs without inducing apoptosis. Coculture of CHE with A375 and A2058 cells also inhibited sphere formation and decreased stemness factors, including Nanog, Oct4, and Sox2. In functional characterizations, we observed that CHE treatment increased both cellular reactive oxygen species (ROS) and mitochondrial ROS, which resulted in a decrease in mitochondrial energy production and sphere formation. Abolishing CHE-induced ROS by N-acetyl-L-cysteine (NAC), a ROS scavenger, reversed the inhibitory effects of CHE on sphere formation, suggesting that CHE-induced ROS are the potential cause of the inhibition of sphere formation. In conclusion, CHE may exert its antitumour effect as an antistem cell natural compound, suggesting that selection of the antistem cell effects of natural compounds might be a promising strategy to overcome the poor prognosis of melanoma due to the presence of CSCs.<\/jats:p>","DOI":"10.1155\/2022\/4000733","type":"journal-article","created":{"date-parts":[[2022,6,18]],"date-time":"2022-06-18T19:05:26Z","timestamp":1655579126000},"page":"1-10","source":"Crossref","is-referenced-by-count":2,"title":["Chelerythrine Chloride Inhibits Stemness of Melanoma Cancer Stem-Like Cells (CSCs) Potentially via Inducing Reactive Oxygen Species and Causing Mitochondria Dysfunction"],"prefix":"10.1155","volume":"2022","author":[{"given":"Hong","family":"Li","sequence":"first","affiliation":[{"name":"Medical Cosmetology Department of Chengdu Second People\u2019s Hospital, Chengdu 610041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"He","sequence":"additional","affiliation":[{"name":"Medical Cosmetology Department of Chengdu Second People\u2019s Hospital, Chengdu 610041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengyu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Medical Cosmetology Department of Chengdu Second People\u2019s Hospital, Chengdu 610041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Liu","sequence":"additional","affiliation":[{"name":"Medical Cosmetology Department of Chengdu Second People\u2019s Hospital, Chengdu 610041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Chen","sequence":"additional","affiliation":[{"name":"Medical Cosmetology Department of Chengdu Second People\u2019s Hospital, Chengdu 610041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5231-586X","authenticated-orcid":true,"given":"Xuewen","family":"Xu","sequence":"additional","affiliation":[{"name":"Aesthetic and Plastic Burn Surgery Department of West China Hospital, Sichuan University, Chengdu 610041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.3109\/07357907.2014.958231"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2015.04.018"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2018.08.004"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1038\/nature06489"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-3997"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-018-0757-5"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1093\/noajnl\/vdab076"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-03197-z"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.5114\/wo.2014.47126"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.4172\/2157-7633.1000363"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.rpor.2018.04.005"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X18500490"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2016.1188154"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-018-2317-3"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1177\/1934578X1801301117"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-02222-0"},{"issue":"17","key":"17","first-page":"2745","article-title":"Advance in studies on pharmacological activities of chelerythrine","volume":"38","author":"P. Wang","year":"2013","journal-title":"Zhongguo Zhong Yao Za Zhi"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/j.trim.2016.07.003"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2015.05.031"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2005.05.037"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0175466"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-011-0513-5"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2017.03.009"},{"issue":"2","key":"24","first-page":"737","article-title":"In vitro and in vivo activity of protein kinase C inhibitor chelerythrine chloride induces tumor cell toxicity and growth delay in vivo","volume":"6","author":"S. J. Chmura","year":"2000","journal-title":"Clinical Cancer Research"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-006-0109-x"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2019.04.031"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2003.08.007"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25010224"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2017.02.020"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4402233"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-09-0113"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M905336199"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)41866-2"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5382.1509"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e14-04-0916"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.2001.1446"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2008.02.007"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.644179"}],"container-title":["Computational and Mathematical Methods in Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/cmmm\/2022\/4000733.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/cmmm\/2022\/4000733.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/cmmm\/2022\/4000733.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,18]],"date-time":"2022-06-18T19:05:32Z","timestamp":1655579132000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/cmmm\/2022\/4000733\/"}},"subtitle":[],"editor":[{"given":"Jialiang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2022,6,18]]},"references-count":38,"alternative-id":["4000733","4000733"],"URL":"https:\/\/doi.org\/10.1155\/2022\/4000733","relation":{},"ISSN":["1748-6718","1748-670X"],"issn-type":[{"value":"1748-6718","type":"electronic"},{"value":"1748-670X","type":"print"}],"subject":[],"published":{"date-parts":[[2022,6,18]]}}}