{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T11:05:00Z","timestamp":1785841500370,"version":"3.56.0"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,7,6]],"date-time":"2022-07-06T00:00:00Z","timestamp":1657065600000},"content-version":"vor","delay-in-days":186,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"General Project of Technology Innovation Application Development of Chongqing Science and Technology Bureau","award":["cstc2019jscx-msxmX0245"],"award-info":[{"award-number":["cstc2019jscx-msxmX0245"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>Osteonecrosis of the femoral head (ONFH) is a disorder that can cause collapse of the femoral head. The damage and dysfunction of femoral head microvascular endothelial cells are related to the pathogenesis of glucocorticoid\u2010induced ONFH. Reports suggest that vitamin B<jats:sub>2<\/jats:sub> can promote osteoblast differentiation and prevent low bone mineral density and prevent reperfusion oxidative injury. To explore the effect and possible molecular mechanism of vitamin B<jats:sub>2<\/jats:sub> on the ONFH and Human Umbilical Vein Endothelial Cells (HUVECs), we performed a rat model of ONFH by dexamethasone. The rats were randomly divided into four groups: control group, vitamin B<jats:sub>2<\/jats:sub> group, dexamethasone group, and dexamethasone combined with vitamin B<jats:sub>2<\/jats:sub> treatment group. HUVECs were used to further prove the role and mechanism of vitamin B<jats:sub>2<\/jats:sub> in vitro. In patients, according to immunohistochemical and qRT\u2010PCR of the femoral head, the angiogenic capacity of the ONFH femoral head is compromised. In vivo, it showed that vitamin B<jats:sub>2<\/jats:sub> could inhibit glucocorticoid\u2010induced ONFH\u2010like changes in rats by suppressing cell apoptosis, promoting the regeneration of blood vessels, and increasing bone mass. According to in vitro results, vitamin B<jats:sub>2<\/jats:sub> could induce the migration of HUVECs, enhance the expression of angiogenesis\u2010related factors, and inhibit glucocorticoid\u2010induced apoptosis. The underlying mechanism may be that vitamin B<jats:sub>2<\/jats:sub> activates the PI3K signaling pathway. Vitamin B<jats:sub>2<\/jats:sub> alleviated dexamethasone\u2010induced ONFH, and vitamin B<jats:sub>2<\/jats:sub> could promote the proliferation and migration of HUVECs and inhibit their apoptosis by activating the PI3K\/Akt signaling pathway. Vitamin B<jats:sub>2<\/jats:sub> may be a potentially effective treatment for ONFH.<\/jats:p>","DOI":"10.1155\/2022\/4006184","type":"journal-article","created":{"date-parts":[[2022,7,6]],"date-time":"2022-07-06T20:50:09Z","timestamp":1657140609000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Vitamin B<sub>2<\/sub> Prevents Glucocorticoid\u2010Caused Damage of Blood Vessels in Osteonecrosis of the Femoral 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