{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T03:43:33Z","timestamp":1784691813538,"version":"3.55.0"},"reference-count":46,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T00:00:00Z","timestamp":1652918400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010849","name":"Health and Family Planning Commission of Wuhan Municipality","doi-asserted-by":"publisher","award":["WX19Q15"],"award-info":[{"award-number":["WX19Q15"]}],"id":[{"id":"10.13039\/501100010849","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010849","name":"Health and Family Planning Commission of Wuhan Municipality","doi-asserted-by":"publisher","award":["2017J01383"],"award-info":[{"award-number":["2017J01383"]}],"id":[{"id":"10.13039\/501100010849","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Nature Science Fund of Fujian Province","award":["WX19Q15"],"award-info":[{"award-number":["WX19Q15"]}]},{"name":"Nature Science Fund of Fujian Province","award":["2017J01383"],"award-info":[{"award-number":["2017J01383"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Analytical Cellular Pathology"],"published-print":{"date-parts":[[2022,5,19]]},"abstract":"<jats:p>Objective. Clear cell renal cell carcinoma (ccRCC) is the major histopathological subtype of renal cancer, and ferroptosis is implicated in the pathogenesis of ccRCC. The present study was aimed at investigating the role and underlying mechanisms of microRNA-4735-3p (miR-4735-3p) in ccRCC. Methods. Human ccRCC cell lines were transfected with the miR-4735-3p mimic or inhibitor to manipulate the expression of miR-4735-3p. Cell proliferation, colony formation, cell migration, cell invasion, cell death, oxidative stress, lipid peroxidation, and iron metabolism were determined. To validate the necessity of solute carrier family 40 member 1 (SLC40A1), human ccRCC cell lines were overexpressed with SLC40A1 using adenoviral vectors. Results. miR-4735-3p expression was reduced in human ccRCC tissues and cell lines but elevated upon ferroptotic stimulation. The miR-4735-3p mimic increased, while the miR-4735-3p inhibitor decreased oxidative stress, lipid peroxidation, iron overload, and ferroptosis of human ccRCC cell lines. Mechanistic studies identified SLC40A1 as a direct target of miR-4735-3p, and SLC40A1 overexpression significantly attenuated iron overload and ferroptosis in the miR-4735-3p mimic-treated human ccRCC cell lines. Conclusion. miR-4735-3p facilitates ferroptosis and tumor suppression in ccRCC by targeting SLC40A1.<\/jats:p>","DOI":"10.1155\/2022\/4213401","type":"journal-article","created":{"date-parts":[[2022,5,20]],"date-time":"2022-05-20T06:05:20Z","timestamp":1653026720000},"page":"1-12","source":"Crossref","is-referenced-by-count":28,"title":["MicroRNA-4735-3p Facilitates Ferroptosis in Clear Cell Renal Cell Carcinoma by Targeting SLC40A1"],"prefix":"10.1155","volume":"2022","author":[{"given":"Cairong","family":"Zhu","sequence":"first","affiliation":[{"name":"Hemodialysis Room, The 7th Hospital of Wuhan, Wuhan, 430071 Hubei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiquan","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Stomatology, First People\u2019s Hospital of Zaoyang City, Zaoyang, 441100 Hubei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430014 Hubei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8435-0857","authenticated-orcid":true,"given":"Tianqi","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Urology, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, 363000 Fujian, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haili","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Urology, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, 363000 Fujian, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenqiang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Urology, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, 363000 Fujian, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8839-2576","authenticated-orcid":true,"given":"Fen","family":"Ai","sequence":"additional","affiliation":[{"name":"Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430014 Hubei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8031-8590","authenticated-orcid":true,"given":"Shijiao","family":"Zheng","sequence":"additional","affiliation":[{"name":"Hemodialysis Room, The 7th Hospital of Wuhan, Wuhan, 430071 Hubei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-012414-040306"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2007.03.035"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-020-00644-4"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-019-0380-z"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2021.102122"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2021.102006"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2020.09.015"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.7150\/thno.60233"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2021.09.014"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19051309"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2021.102021"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-018-0315-z"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2021.650416"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12113150"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.676142"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-019-0352-3"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-019-0393-7"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2021.102149"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2021.102131"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-016-2194-1"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.390"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-021-00743-w"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-021-00779-y"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-03306-y"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13205160"},{"key":"26","first-page":"3083","article-title":"Low expression of miR-99b promotes progression of clear cell renal cell carcinoma by up-regulating IGF1R\/Akt\/mTOR signaling","volume":"13","author":"T. Lin","year":"2020","journal-title":"International Journal of Clinical and Experimental Pathology"},{"issue":"8","key":"27","first-page":"3714","article-title":"Suppression of miR-4735-3p in androgen receptor-expressing prostate cancer cells increases cell death during chemotherapy","volume":"9","author":"W. Zhou","year":"2017","journal-title":"American Journal of Translational Research"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1080\/15384047.2018.1538613"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.28025"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-021-00463-6"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.124"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2021.04.009"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2020.04.011"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-021-00781-4"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-021-00804-0"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-021-00752-9"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.26492"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101747"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-020-00685-9"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-020-0473-8"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2021.102051"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2021.101910"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21590"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.14740\/wjon1279"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2018.11.021"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2018.12.008"}],"container-title":["Analytical Cellular Pathology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/acp\/2022\/4213401.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/acp\/2022\/4213401.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/acp\/2022\/4213401.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,20]],"date-time":"2022-05-20T06:05:27Z","timestamp":1653026727000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/acp\/2022\/4213401\/"}},"subtitle":[],"editor":[{"given":"Consuelo","family":"Amantini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,5,19]]},"references-count":46,"alternative-id":["4213401","4213401"],"URL":"https:\/\/doi.org\/10.1155\/2022\/4213401","relation":{},"ISSN":["2210-7185","2210-7177"],"issn-type":[{"value":"2210-7185","type":"electronic"},{"value":"2210-7177","type":"print"}],"subject":[],"published":{"date-parts":[[2022,5,19]]}}}