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While most sperm\u2010mediated causes have been investigated in detail, the significance of seminal plasma has been neglected. Herein, we aimed to investigate the possible pathogenic factors leading to decreased sperm motility based on seminal plasma. Semen was collected from normo\u2010 (NOR, <jats:italic>n<\/jats:italic> = 70), idiopathic oligo\u2010 (OLI, <jats:italic>n<\/jats:italic> = 57), and idiopathic asthenozoospermic (AST, <jats:italic>n<\/jats:italic> = 53) patients. Using attenuated total reflection\u2010Fourier transform infrared coupled with chemometrics, distinct differences in the biochemical compositions of nucleic acids, protein structure (amides I, II, and III), lipids, and carbohydrates in seminal plasma of AST were observed when compared to NOR and OLI. Compared with NOR and OLI, the levels of peptide aggregation, protein phosphorylation, unsaturated fatty acid, and lipid to protein ratio were significantly increased in AST; however, the level of lipid saturation was significantly decreased in seminal plasma of AST. Compared with NOR, the levels of ROS, MDA, 8\u2010iso\u2010prostaglandin F2<jats:italic>\u03b1<\/jats:italic> (8\u2010isoPGF2<jats:italic>\u03b1<\/jats:italic>), and the ratio of phospho\u2010AMPK<jats:italic>\u03b1<\/jats:italic>\/AMPK<jats:italic>\u03b1<\/jats:italic>1 were significantly increased in AST; however, the levels of SOD, glutathione S\u2010transferase (GSTs), protein carbonyl derivative (PC), and the ratio of phospho\u2010Rictor\/Rictor were significantly decreased in seminal plasma of AST. Changes of the AMPK\/mTORC2 signaling in the seminal microenvironment possibly induce abnormal glucose and lipid metabolism, which impairs energy production. Oxidative stress potentially damages seminal plasma lipids and proteins, which in turn leads to impaired sperm structure and function. These findings provide evidence that the changes in seminal plasma compositions, oxidative stress, and activation of the AMPK\/mTORC2 signaling contribute to the development of asthenozoospermia.<\/jats:p>","DOI":"10.1155\/2022\/4240490","type":"journal-article","created":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T17:20:09Z","timestamp":1654708809000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The Activated AMPK\/mTORC2 Signaling Pathway Associated with Oxidative Stress in Seminal Plasma Contributes to Idiopathic 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