{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T01:05:08Z","timestamp":1788743108702,"version":"build-2803163510"},"reference-count":25,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,1,15]],"date-time":"2022-01-15T00:00:00Z","timestamp":1642204800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["SQ2020YFF0426358"],"award-info":[{"award-number":["SQ2020YFF0426358"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["81902115"],"award-info":[{"award-number":["81902115"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["SQ2020YFF0426358"],"award-info":[{"award-number":["SQ2020YFF0426358"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81902115"],"award-info":[{"award-number":["81902115"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2022,1,15]]},"abstract":"<jats:p>Background. There is lack of reliable serum biomarkers to reflect the severity of liver necroinflammation for those who suffer autoimmune liver diseases (AILDs). In this study, a previously established patient cohort was used to explore the potential of serum Golgi protein 73 (GP73) as a noninvasive marker of AILD-related liver necroinflammation. Methods. Serum GP73 concentration was measured in a retrospective cohort of 168 AILD patients, which included 74 patients with autoimmune hepatitis (AIH) and 94 with primary biliary cholangitis (PBC) who had undergone liver biopsy. Spearman\u2019s correlation and multivariate analysis were used to evaluate the relationship between serum GP73 and liver necroinflammation. A receiver operating characteristic curve was constructed to evaluate the value of GP73 for the prediction of moderate or severe liver necroinflammation. The diagnostic value of serum GP73 was also compared with that of alkaline phosphatase (ALP) in patients with PBC. Histologically, immunohistochemical analysis was performed to assess hepatic GP73 expression. Results. Both the serum level and hepatic tissue expression of GP73 protein were aberrantly elevated and correlated well with the severity of necroinflammation in both AIH (<jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mtext>rho<\/a:mtext>\n                        <a:mo>=<\/a:mo>\n                        <a:mn>0.655<\/a:mn>\n                     <\/a:math>\n                  <\/jats:inline-formula>, <jats:inline-formula>\n                     <c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                        <c:mi>P<\/c:mi>\n                        <c:mo>&lt;<\/c:mo>\n                        <c:mn>0.001<\/c:mn>\n                     <\/c:math>\n                  <\/jats:inline-formula>) and PBC (<jats:inline-formula>\n                     <e:math xmlns:e=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\">\n                        <e:mtext>rho<\/e:mtext>\n                        <e:mo>=<\/e:mo>\n                        <e:mn>0.547<\/e:mn>\n                     <\/e:math>\n                  <\/jats:inline-formula>, <jats:inline-formula>\n                     <g:math xmlns:g=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\">\n                        <g:mi>P<\/g:mi>\n                        <g:mo>&lt;<\/g:mo>\n                        <g:mn>0.001<\/g:mn>\n                     <\/g:math>\n                  <\/jats:inline-formula>) patients. The results here suggested that serum GP73 could be an independent biomarker to reflect the severity of liver necroinflammation. The AUROCs for GP73 to predict moderate necroinflammation (\u2265G2) and severe necroinflammation (\u2265G3) in patients with AIH were 0.828 and 0.832, respectively. Moreover, the AUROCs of serum GP73 for the identification of moderate necroinflammation (\u2265G2) (<jats:inline-formula>\n                     <i:math xmlns:i=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\">\n                        <i:mtext>AUROC<\/i:mtext>\n                        <i:mo>=<\/i:mo>\n                        <i:mn>0.820<\/i:mn>\n                     <\/i:math>\n                  <\/jats:inline-formula>, <jats:inline-formula>\n                     <k:math xmlns:k=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\">\n                        <k:mi>P<\/k:mi>\n                        <k:mo>&lt;<\/k:mo>\n                        <k:mn>0.001<\/k:mn>\n                     <\/k:math>\n                  <\/jats:inline-formula>) and severe necroinflammation (\u2265G3) (<jats:inline-formula>\n                     <m:math xmlns:m=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M7\">\n                        <m:mtext>AUROC<\/m:mtext>\n                        <m:mo>=<\/m:mo>\n                        <m:mn>0.803<\/m:mn>\n                     <\/m:math>\n                  <\/jats:inline-formula>, <jats:inline-formula>\n                     <o:math xmlns:o=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M8\">\n                        <o:mi>P<\/o:mi>\n                        <o:mo>&lt;<\/o:mo>\n                        <o:mn>0.001<\/o:mn>\n                     <\/o:math>\n                  <\/jats:inline-formula>) were superior to those of ALP (\u2265G2: <jats:inline-formula>\n                     <q:math xmlns:q=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M9\">\n                        <q:mtext>AUROC<\/q:mtext>\n                        <q:mo>=<\/q:mo>\n                        <q:mn>0.607<\/q:mn>\n                     <\/q:math>\n                  <\/jats:inline-formula>, <jats:inline-formula>\n                     <s:math xmlns:s=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M10\">\n                        <s:mi>P<\/s:mi>\n                        <s:mo>=<\/s:mo>\n                        <s:mn>0.028<\/s:mn>\n                     <\/s:math>\n                  <\/jats:inline-formula> and \u2265G3: <jats:inline-formula>\n                     <u:math xmlns:u=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M11\">\n                        <u:mtext>AUROC<\/u:mtext>\n                        <u:mo>=<\/u:mo>\n                        <u:mn>0.559<\/u:mn>\n                     <\/u:math>\n                  <\/jats:inline-formula>, <jats:inline-formula>\n                     <w:math xmlns:w=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M12\">\n                        <w:mi>P<\/w:mi>\n                        <w:mo>=<\/w:mo>\n                        <w:mn>0.357<\/w:mn>\n                     <\/w:math>\n                  <\/jats:inline-formula>) in patients with PBC. Mechanically, interlukin-6 (IL-6), the proinflammatory and prohepatic regenerating cytokine, could transcriptionally upregulate GP73 gene expression. Conclusion. Serum GP73 is a potential noninvasive biomarker to evaluate the severity of liver necroinflammation in patients with AILDs.<\/jats:p>","DOI":"10.1155\/2022\/4253566","type":"journal-article","created":{"date-parts":[[2022,1,16]],"date-time":"2022-01-16T03:05:09Z","timestamp":1642302309000},"page":"1-7","source":"Crossref","is-referenced-by-count":16,"title":["Diagnostic Value of Serum Golgi Protein 73 for Liver Inflammation in Patients with Autoimmune Hepatitis and Primary Biliary Cholangitis"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5341-1714","authenticated-orcid":true,"given":"Mingjie","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Anatomy and Embryology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4434-9541","authenticated-orcid":true,"given":"Leijie","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Infectious Disease Center, School of Basic Medicine, Peking University Health Science Center, 38 Xueyuan Road, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2398-7448","authenticated-orcid":true,"given":"Jianwen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Infectious Disease Center, School of Basic Medicine, Peking University Health Science Center, 38 Xueyuan Road, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6573-4318","authenticated-orcid":true,"given":"Yanna","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Infectious Disease Center, School of Basic Medicine, Peking University Health Science Center, 38 Xueyuan Road, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4668-5677","authenticated-orcid":true,"given":"Shuhong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Hepatology, The Fifth Medical Center of PLA General Hospital, 100039, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4345-2149","authenticated-orcid":true,"given":"Jingmin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pathology and Hepatology, The Fifth Medical Center of PLA General Hospital, 100039, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1832-3209","authenticated-orcid":true,"given":"Fengmin","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Infectious Disease Center, School of Basic Medicine, Peking University Health Science Center, 38 Xueyuan Road, Beijing 100191, China"},{"name":"Center of Precision Medicine, Academy of Medical Sciences, Zhengzhou University, Zhengzhou, China"},{"name":"Peking University People\u2019s Hospital, Peking University Hepatology Institute, Peking University Health Science Center, 11 South Xizhimen Street, Beijing 100044, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.25599"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2004.01.009"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(01)00155-6"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2002.32525"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053862"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2011.640"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2011.097"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25838"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2009.05.029"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000000659"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2014.08.010"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-010-0278-5"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2010.214916"},{"key":"14","doi-asserted-by":"crossref","first-page":"2644","DOI":"10.3892\/mmr.2014.3114","article-title":"Serum Golgi protein 73 levels and liver pathological grading in cases of chronic hepatitis B","volume":"11","author":"Z. 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