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This obligate\u2010human pathogen has developed a number of defenses against both innate and adaptive immune responses during infection, some of which are mediated by the pathogen\u2019s proteins. Hence, the uncharacterized proteins of <jats:italic>N. gonorrhoeae<\/jats:italic> can be annotated to get insight into the unique functions of this organism related to its pathogenicity and to find a more efficient therapeutic target. <jats:italic>Methods<\/jats:italic>. In this study, a hypothetical protein (HP) of <jats:italic>N. gonorrhoeae<\/jats:italic> was chosen for analysis and an in\u2010silico approach was used to explore various properties such as physicochemical characteristics, subcellular localization, secondary structure, 3D structures, and functional annotation of that HP. Finally, a molecular docking analysis was performed to design an epitope\u2010based vaccine against that HP. <jats:italic>Results<\/jats:italic>. This study has identified the potential role of the chosen HP of <jats:italic>N. gonorrhoeae<\/jats:italic> in plasmid transfer, cell cycle control, cell division, and chromosome partitioning. Acidic nature, thermal stability, cytoplasmic localization of the protein, and some of its other physicochemical properties have also been identified through this study. Molecular docking analysis has demonstrated that one of the T cell epitopes of the protein has a significant binding affinity with the human leukocyte antigen HLA\u2010B\u221715\u2009:\u200901. <jats:italic>Conclusions<\/jats:italic>. The in\u2010silico characterization of this protein will help us understand molecular mechanism of action of <jats:italic>N. gonorrhoeae<\/jats:italic> and get an insight into novel therapeutic identification processes. This research will, therefore, enhance our knowledge to find new medications to tackle this potential threat to humankind.<\/jats:p>","DOI":"10.1155\/2022\/4302625","type":"journal-article","created":{"date-parts":[[2022,9,5]],"date-time":"2022-09-05T23:50:13Z","timestamp":1662421813000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Structural and Functional Annotation and Molecular Docking Analysis of a Hypothetical Protein from <i>Neisseria gonorrhoeae<\/i>: An In\u2010Silico Approach"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6463-9418","authenticated-orcid":false,"given":"Lincon","family":"Mazumder","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4880-9311","authenticated-orcid":false,"given":"Md. 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