{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T19:07:56Z","timestamp":1784833676018,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T00:00:00Z","timestamp":1663632000000},"content-version":"vor","delay-in-days":262,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005317","name":"Kermanshah University of Medical Sciences","doi-asserted-by":"publisher","award":["91274"],"award-info":[{"award-number":["91274"]}],"id":[{"id":"10.13039\/501100005317","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>Today, the spread of vancomycin\u2010resistant strains isolated from <jats:italic>Enterococcus faecalis<\/jats:italic> (<jats:italic>E. faecalis<\/jats:italic>) has become a major health concern worldwide. Therefore, it is essential to provide a rapid and sensitive assay for identifying <jats:italic>vanA<\/jats:italic> gene for timely and appropriate antimicrobial control of resistant enterococcal infections. For this purpose, a cross\u2010sectional study was performed on different clinical specimens of enterococci from Imam Reza hospital, Kermanshah, Iran. The antimicrobial susceptibility testing was determined by disk diffusion and MIC methods. Triplex\u2010PCR and duplex\u2010LAMP assays were also used to identify <jats:italic>vanA E. faecalis<\/jats:italic> resistance gene isolates. The results of this study shown that out of 108 <jats:italic>Enterococcus<\/jats:italic> isolates, 86, 18, 2, 1, and one isolates of <jats:italic>E. faecalis<\/jats:italic>, <jats:italic>E. faecium<\/jats:italic>, <jats:italic>E. avium<\/jats:italic>, <jats:italic>E. psudoavium<\/jats:italic>, and <jats:italic>E. raffinosus<\/jats:italic> were identified, respectively. On the other hand, <jats:italic>E. faecalis<\/jats:italic> was confirmed in 87 and 88 isolates using duplex\u2010LAMP and triplex PCR, respectively. The LAMP primer set designed in this study can reliably identify seven distinct regions of the <jats:italic>vanA<\/jats:italic> gene, and finally the sensitivity, specificity, and the positive and negative predictive values of LAMP assay were shown to be 94.19%, 72.73%, 76.19%, and 93.10%, respectively. In general, sample processing, isothermal reaction and result reporting were completed using the LAMP assay in 75 minutes. Our findings suggest that LAMP assay has been approved as an alternative to the vancomycin resistance <jats:italic>Enterococcus<\/jats:italic> genotype (<jats:italic>vanA<\/jats:italic> and <jats:italic>vanB<\/jats:italic>) compared to other methods and has the advantage of being rapid, time\u2010consuming, and easy for diagnosis.<\/jats:p>","DOI":"10.1155\/2022\/4384196","type":"journal-article","created":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T14:50:05Z","timestamp":1663685405000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Rapid Detection of <i>vanA<\/i> Resistance Gene from <i>E. faecalis<\/i> Clinical Isolates Using Duplex Loop\u2010Mediated Isothermal Amplification and Triplex PCR Assay"],"prefix":"10.1155","volume":"2022","author":[{"given":"Mohsen","family":"Azizi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid","family":"Motamedi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3784-2645","authenticated-orcid":false,"given":"Hadi","family":"Hossainpour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramin","family":"Abiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahsa","family":"Kashef","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamal","family":"Ahmadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jale","family":"Moradi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4963-2485","authenticated-orcid":false,"given":"Amirhooshang","family":"Alvandi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,20]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781555816865"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8081118"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2017.0147"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00211-16"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.11.2180"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/644790"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198807213190307"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00788"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1139\/cjm-2019-0309"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-62371-7"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-022-01818-7"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.28025"},{"key":"e_1_2_10_13_2","volume-title":"Textbook of diagnostic microbiology-e-book","author":"Mahon C. 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