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We have previously standardised a micro-PET methodology in mice by intravenously administering [\n                    <jats:sup>18<\/jats:sup>\n                    F]-FDOPA via jugular vein cannulation and assessment of striatal dopamine synthesis capacity, indexed as the influx rate constant [Formula: see text] of [\n                    <jats:sup>18<\/jats:sup>\n                    F]-FDOPA, using an extended graphical Patlak analysis with the cerebellum as a reference region. This enables a direct comparison between preclinical and clinical output values. However, chronic intravenous catheters are technically difficult to maintain for longitudinal studies. Hence, in this study, intraperitoneal administration of [\n                    <jats:sup>18<\/jats:sup>\n                    F]-FDOPA was evaluated as a less-invasive alternative that facilitates longitudinal imaging. Our experiments comprised the following assessments: (i) comparison of [\n                    <jats:sup>18<\/jats:sup>\n                    F]-FDOPA uptake between intravenous and intraperitoneal radiotracer administration and optimisation of the time window used for extended Patlak analysis, (ii) comparison of [Formula: see text] in a within-subject design of both administration routes, (iii) test-retest evaluation of [Formula: see text] in a within-subject design of intraperitoneal radiotracer administration, and (iv) validation of [Formula: see text] estimates by comparing the two administration routes in a mouse model of hyperdopaminergia induced by subchronic ketamine. Our results demonstrate that intraperitoneal [\n                    <jats:sup>18<\/jats:sup>\n                    F]-FDOPA administration resulted in good brain uptake, with no significant effect of administration route on [Formula: see text] estimates (intraperitoneal: [Formula: see text], intravenous: [Formula: see text], [Formula: see text]) and similar coefficient of variation (intraperitoneal: 19.6%; intravenous: 18.4%). The technique had a moderate test-retest validity ([Formula: see text], [Formula: see text]) and thus supports longitudinal studies. Following subchronic ketamine administration, elevated [Formula: see text] as compared to control condition was measured with a large effect size for both methods (intraperitoneal: Cohen\u2019s [Formula: see text]; intravenous: Cohen\u2019s [Formula: see text]), providing further evidence that ketamine has lasting effects on the dopamine system, which could contribute to its therapeutic actions and\/or abuse liability.\n                  <\/jats:p>","DOI":"10.1155\/2022\/4419221","type":"journal-article","created":{"date-parts":[[2022,10,28]],"date-time":"2022-10-28T10:35:09Z","timestamp":1666953309000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Evaluation of Intraperitoneal [\n                    <sup>18<\/sup>\n                    F]-FDOPA Administration for Micro-PET Imaging in Mice and Assessment of the Effect of Subchronic Ketamine Dosing on Dopamine Synthesis Capacity"],"prefix":"10.1177","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5814-0899","authenticated-orcid":false,"given":"Els F.","family":"Halff","sequence":"first","affiliation":[{"name":"Institute of PsychiatryPsychology and NeuroscienceKing\u2019s College LondonLondonUK"},{"name":"Psychiatric Imaging GroupMRC London Institute of Medical SciencesLondonUK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7499-1714","authenticated-orcid":false,"given":"Sridhar","family":"Natesan","sequence":"additional","affiliation":[{"name":"Institute of PsychiatryPsychology and NeuroscienceKing\u2019s College LondonLondonUK"},{"name":"Psychiatric Imaging GroupMRC London Institute of Medical SciencesLondonUK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Bonsall","sequence":"additional","affiliation":[{"name":"Psychiatric Imaging GroupMRC London Institute of Medical 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MedicineImperial College LondonLondonUK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sac-Pham","family":"Tang","sequence":"additional","affiliation":[{"name":"InvicroBurlington DanesHammersmith HospitalLondonUK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2263-1722","authenticated-orcid":false,"given":"Laura J.","family":"Riggall","sequence":"additional","affiliation":[{"name":"Psychiatric Imaging GroupMRC London Institute of Medical SciencesLondonUK"},{"name":"Institute of Clinical SciencesFaculty of MedicineImperial College LondonLondonUK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger N.","family":"Gunn","sequence":"additional","affiliation":[{"name":"InvicroBurlington DanesHammersmith HospitalLondonUK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine E.","family":"Irvine","sequence":"additional","affiliation":[{"name":"Institute of Clinical SciencesFaculty of 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