{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T15:09:55Z","timestamp":1778857795234,"version":"3.51.4"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,7,28]],"date-time":"2022-07-28T00:00:00Z","timestamp":1658966400000},"content-version":"vor","delay-in-days":208,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. To conduct a comprehensive bioinformatics analysis on the transcriptome signatures of Toll\u2010like receptors (TLRs) in pan\u2010cancer. <jats:italic>Materials and methods<\/jats:italic>. A total of 11,057 tissues consisting of 33 types of carcinoma in The Cancer Genome Atlas (TCGA) were retrieved, and then we further explored the correlation between TLRs\u2019 expression with tumorigenesis, immune infiltration, and drug sensitivity. We conducted a comprehensive bioinformatics analysis on TLR1 to 10 in pan\u2010cancer, including differential expression analysis between normal and tumor tissues, differential immune subtype correlation, survival analysis, tumor immune infiltration estimating, stemness indices correlation, and drug responses correlation. <jats:italic>Results<\/jats:italic>. TLR2 was highly expressed in most types of tumors. TLR9 was hardly expressed compared to other TLR genes, which lead to TLR9 showing less correlation with both immune\u2010estimate scores and stromal\u2010estimate scores. All the TLRs were related with immune subtype of tumor samples that all of them were differentially expressed in differential immune subtype samples. The expression of TLRs was positively related with immune\u2010estimate scores and stromal\u2010estimate scores in almost all types of tumor. The expression of TLRs was negatively correlated with mRNA expression\u2010based stemness scores (RNAss) in nearly almost type of tumors except kidney renal clear cell carcinoma (KIRC) and also negatively correlated with DNA methylation\u2010based stemness scores (DNAss) in many types of tumors except adrenocortical carcinoma (ACC), cholangiocarcinoma (CHOL), KIRC, acute myeloid leukemia (LAML), low\u2010grade glioma (LGG), testicular germ cell tumors (TGCT), thyroid carcinoma (THCA), thymoma (THYM), and uveal melanoma (UVM). The expression of TLR9 was significantly positively correlated with the drug sensitivity of fluphenazine, alectinib, carmustine, and 7\u2212hydroxystaurosporine. TLR7 was significantly positively correlated with the drug sensitivity of alectinib. <jats:italic>Conclusions<\/jats:italic>. Our study reveals the significant role of TLRs family in pan\u2010cancer and provides potential therapeutic strategies of cancer.<\/jats:p>","DOI":"10.1155\/2022\/4436646","type":"journal-article","created":{"date-parts":[[2022,7,28]],"date-time":"2022-07-28T22:50:49Z","timestamp":1659048649000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Comprehensive Bioinformatics Analysis of Toll\u2010Like Receptors (TLRs) in 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