{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:33:48Z","timestamp":1791329628875,"version":"4.3.1"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,7,31]],"date-time":"2022-07-31T00:00:00Z","timestamp":1659225600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Sichuan Provincial Science and Technology Department Key Research and Development Projects","award":["2020YFS0141"],"award-info":[{"award-number":["2020YFS0141"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2022,7,31]]},"abstract":"<jats:p>In order to test the effectiveness of nanoparticle- (NP-) loaded bone morphogenetic protein 2 (BMP-2) in chronic osteomyelitis (CO) complicated with bone defect, a new nanodrug delivery system composed of mesoporous silica NP (MSN) and chitosan were used to load BMP-2 and transfer it to the target region. Bone marrow mesenchymal stem cells (BMSCs) were purchased and cultivated to detect the osteogenesis of chitosan-MSN (Chi-MSN) and polylactic acid glycolic acid (PLGA) delivery system. In addition, the osteogenesis of Chi-MSN was further determined by constructing a bone defect mouse model. In physicochemical property test, we found Chi-MSN NPs could effectively maintain stability in vivo and had pH response characteristics. As a result, the release efficiency of dexamethasone (Dex) and BMP-2 in the environment with pH\u20097.4 was less, while it increased significantly in pH 6, so as to reduce the BMP-2 and Dex loss during transportation in vivo. Otherwise, we found that the permeation efficiency of Chi-MSN was significantly higher than that of PLGA delivery system, so as to effectively transport BMP-2 and Dex to action target. In the BMSC test, we found that Chi-MSN could better promote their activity and osteogenesis, and the expression of osteogenesis-related genes (runt-related transcription factor 2 (RUNX-2), osteopontine (OPN), alkaline phosphatase (ALP), and osteopontine (OCN)) in the Chi-MSN group was higher. In the bone defect mouse model test, we also found obviously increased bone trabecula number and thickness by Chi-MSN, contributing to better repair of bone defects. Therefore, BMP-2@Chi-MSN may be a better choice for the therapy of CO complicated with bone defect in the future.<\/jats:p>","DOI":"10.1155\/2022\/4450196","type":"journal-article","created":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T04:33:43Z","timestamp":1659328423000},"page":"1-11","source":"Crossref","is-referenced-by-count":18,"title":["Application of Mesoporous Silica Nanoparticle-Chitosan-Loaded BMP-2 in the Repair of Bone Defect in Chronic Osteomyelitis"],"prefix":"10.1155","volume":"2022","author":[{"given":"Min","family":"Yi","sequence":"first","affiliation":[{"name":"Department of Orthopedics, Orthopedic Research Institute, West China Hospital, Sichuan University, Chengdu, 610041 Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Nie","sequence":"additional","affiliation":[{"name":"Engineering Research Center in Biomaterials, Sichuan University, Chengdu, 610041 Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengyun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Orthopedic Research Institute, West China Hospital, Sichuan University, Chengdu, 610041 Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9794-6809","authenticated-orcid":true,"given":"Bin","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Orthopedic Research Institute, West China Hospital, Sichuan University, Chengdu, 610041 Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cden.2016.12.001"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir842"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.pcl.2018.04.003"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD004439.pub3"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1007\/s11926-017-0645-9"},{"issue":"2","key":"6","first-page":"321","article-title":"Bone marrow stromal cell (BMSC) and skeletal aging: role of telomerase enzyme","volume":"27","author":"H. 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